PMID- 30427836 OWN - NLM STAT- MEDLINE DCOM- 20190410 LR - 20190410 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 13 IP - 11 DP - 2018 TI - The kinetics of gut microbial community composition in patients with irritable bowel syndrome following fecal microbiota transplantation. PG - e0194904 LID - 10.1371/journal.pone.0194904 [doi] LID - e0194904 AB - BACKGROUND: Gut microbiota alterations are important in irritable bowel syndrome (IBS). The aim was to investigate the effect of fecal microbiota transplantation (FMT) on gut microbiota and the symptoms in patients with IBS. MATERIAL AND METHODS: The study included 13 IBS patients according to Rome III criteria and 13 healthy donors. Freshly donated feces were administered to the descending part of the duodenum via a gastroscope. Feces were collected from donors and patients before FMT, and from the patients at 1, 3 and 12 weeks and donors and patients at 20/28 weeks after FMT. Microbiota analysis was performed using GA-map Dysbiosis test (Genetic Analysis AS, Oslo, Norway). The patients completed the following questionnaires before and at the aforementioned weeks after FMT: IBS Symptom Questionnaire (IBS-SQ), IBS-Symptom Severity Scoring system (IBS-SSS), Short Form of Nepean Dyspepsia Index (SF-NDI), Bristol stool form scale, the Eysenck Personality Questionnaire-Neuroticism and Hospital Anxiety and Depression. RESULTS: Donors and IBS patients had significantly different bacterial strain signals before FMT (Ruminococcus gnavus, Actinobacteria and Bifidobacteria) that became non-significant after 3 weeks following FMT. The changes in gut microbiota were similar between donors and patients at 20/28 weeks after FMT. Thus, patients' microbiota profiles became more-or-less similar to donors. The scores of all the questionnaires were significantly improved at all time points following FMT. No reported adverse effects. CONCLUSIONS: FMT was associated with a change in gut microbiota and improvement in IBS symptoms and quality of life lasting for up to 28 weeks. TRIAL REGISTRATION: ClinicalTrials.gov ID: NCT03333291. FAU - Mazzawi, Tarek AU - Mazzawi T AUID- ORCID: 0000-0001-7983-3707 AD - Section of Gastroenterology, Department of Medicine, Haukeland University Hospital, Bergen, Norway. AD - Norwegian Competence Centre for Functional Gastro-Intestinal Disorders, Section of Gastroenterology, Department of Medicine, Haukeland University Hospital, Bergen, Norway. AD - Center for Nutrition, Department of Clinical Medicine, University of Bergen, Bergen, Norway. FAU - Lied, Gulen Arslan AU - Lied GA AD - Section of Gastroenterology, Department of Medicine, Haukeland University Hospital, Bergen, Norway. AD - Norwegian Competence Centre for Functional Gastro-Intestinal Disorders, Section of Gastroenterology, Department of Medicine, Haukeland University Hospital, Bergen, Norway. AD - Center for Nutrition, Department of Clinical Medicine, University of Bergen, Bergen, Norway. FAU - Sangnes, Dag Andre AU - Sangnes DA AD - Section of Gastroenterology, Department of Medicine, Haukeland University Hospital, Bergen, Norway. FAU - El-Salhy, Magdy AU - El-Salhy M AD - Norwegian Competence Centre for Functional Gastro-Intestinal Disorders, Section of Gastroenterology, Department of Medicine, Haukeland University Hospital, Bergen, Norway. AD - Center for Nutrition, Department of Clinical Medicine, University of Bergen, Bergen, Norway. AD - Section of Gastroenterology, Department of Medicine, Stord Hospital, Helse-Fonna, Stord, Norway. FAU - Hov, Johannes R AU - Hov JR AD - Norwegian PSC Research Center, Section of Gastroenterology and Research Institute of Internal Medicine, Division of Surgery, Inflammatory Diseases and Transplantation, Oslo University Hospital and University of Oslo, Oslo, Norway. FAU - Gilja, Odd Helge AU - Gilja OH AD - Section of Gastroenterology, Department of Medicine, Haukeland University Hospital, Bergen, Norway. AD - Norwegian Competence Centre for Functional Gastro-Intestinal Disorders, Section of Gastroenterology, Department of Medicine, Haukeland University Hospital, Bergen, Norway. AD - Center for Nutrition, Department of Clinical Medicine, University of Bergen, Bergen, Norway. FAU - Hatlebakk, Jan Gunnar AU - Hatlebakk JG AD - Section of Gastroenterology, Department of Medicine, Haukeland University Hospital, Bergen, Norway. AD - Norwegian Competence Centre for Functional Gastro-Intestinal Disorders, Section of Gastroenterology, Department of Medicine, Haukeland University Hospital, Bergen, Norway. AD - Center for Nutrition, Department of Clinical Medicine, University of Bergen, Bergen, Norway. FAU - Hausken, Trygve AU - Hausken T AD - Section of Gastroenterology, Department of Medicine, Haukeland University Hospital, Bergen, Norway. AD - Norwegian Competence Centre for Functional Gastro-Intestinal Disorders, Section of Gastroenterology, Department of Medicine, Haukeland University Hospital, Bergen, Norway. AD - Center for Nutrition, Department of Clinical Medicine, University of Bergen, Bergen, Norway. LA - eng SI - ClinicalTrials.gov/NCT03333291 PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20181114 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Adolescent MH - Adult MH - Aged MH - *Fecal Microbiota Transplantation MH - Feces/microbiology MH - Female MH - Gastrointestinal Microbiome/*physiology MH - Humans MH - Irritable Bowel Syndrome/microbiology/*therapy MH - Male MH - *Microbiota MH - Middle Aged MH - Quality of Life MH - Young Adult PMC - PMC6235238 COIS- The authors have declared that no competing interests exist. EDAT- 2018/11/15 06:00 MHDA- 2019/04/11 06:00 PMCR- 2018/11/14 CRDT- 2018/11/15 06:00 PHST- 2017/11/04 00:00 [received] PHST- 2018/09/28 00:00 [accepted] PHST- 2018/11/15 06:00 [entrez] PHST- 2018/11/15 06:00 [pubmed] PHST- 2019/04/11 06:00 [medline] PHST- 2018/11/14 00:00 [pmc-release] AID - PONE-D-17-36974 [pii] AID - 10.1371/journal.pone.0194904 [doi] PST - epublish SO - PLoS One. 2018 Nov 14;13(11):e0194904. doi: 10.1371/journal.pone.0194904. eCollection 2018.