PMID- 30431073
OWN - NLM
STAT- MEDLINE
DCOM- 20190314
LR  - 20211201
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 54
IP  - 1
DP  - 2019 Jan
TI  - miR‑494.3p expression in synovial sarcoma: Role of CXCR4 as a potential target 
      gene.
PG  - 361-369
LID - 10.3892/ijo.2018.4627 [doi]
AB  - Synovial sarcoma (SS) is a rare tumour, with dismal survival when metastasis 
      occurs. SS contains a characteristic translocation (X;18)(p11;q11) and the fusion 
      genes appear to be mutually exclusive and concordant in primary and metastatic 
      tumours. Novel prognostic and predictive factors are required. The C‑X‑C motif 
      chemokine ligand 12 (CXCL12)/C‑X‑C chemokine receptor 4 (CXCR4) axis is involved 
      in tumour development and metastatic spread in many types of cancer and previous 
      data have demonstrated a pivotal role of CXCR4 in SS cell migration and invasion. 
      Bioinformatics and biological data indicated CXCR4 is a possible candidate target 
      of miR‑494.3p, known to be involved in tumour progression. In this study, we 
      analysed the expression of miR‑494.3p and its potential target, CXCR4, in a 
      series of SS specimens. A significantly lower miR‑494.3p expression was found in 
      the tumour compared to normal tissue associated with higher levels of CXCR4 both 
      at the gene and protein level. The role of CXCR4 as a potential target of 
      miR‑494.3p was assessed in two SS cell lines (SW982 and SYO‑I). Transfection with 
      miR‑494.3p expression plasmid led to a marked decrease in CXCR4 gene and protein 
      expression, concomitant with a transitory decrease in cell proliferation and 
      migration. The SYO‑I cells also responded with an increased apoptotic fraction. 
      The data of this study also demonstrate that the downregulation of miR‑494.3p in 
      SS surgical specimens, concomitant with an increased expression of its potential 
      target, CXCR4, was more evident in the metastatic subset. In vitro experiments 
      confirmed that miR‑494.3p functioned as a tumour suppressor through the 
      involvement of CXCR4 and ongoing studies are directed to better clarify its role 
      in SS therapeutic strategies.
FAU - Pazzaglia, Laura
AU  - Pazzaglia L
AD  - Laboratory of Experimental Oncology, IRCCS, Rizzoli Orthopedic Institute, I‑40136 
      Bologna, Italy.
FAU - Pollino, Serena
AU  - Pollino S
AD  - Laboratory of Experimental Oncology, IRCCS, Rizzoli Orthopedic Institute, I‑40136 
      Bologna, Italy.
FAU - Vitale, Mattia
AU  - Vitale M
AD  - Laboratory of Experimental Oncology, IRCCS, Rizzoli Orthopedic Institute, I‑40136 
      Bologna, Italy.
FAU - Bientinesi, Elisa
AU  - Bientinesi E
AD  - Laboratory of Experimental Oncology, IRCCS, Rizzoli Orthopedic Institute, I‑40136 
      Bologna, Italy.
FAU - Benini, Stefania
AU  - Benini S
AD  - Department of Pathology, IRCCS, Rizzoli Orthopedic Institute, I‑40136 Bologna, 
      Italy.
FAU - Ferrari, Cristina
AU  - Ferrari C
AD  - Laboratory of Experimental Oncology, IRCCS, Rizzoli Orthopedic Institute, I‑40136 
      Bologna, Italy.
FAU - Palmerini, Emanuela
AU  - Palmerini E
AD  - Chemotherapy Unit, IRCCS, Rizzoli Orthopedic Institute, I‑40136 Bologna, Italy.
FAU - Gambarotti, Marco
AU  - Gambarotti M
AD  - Department of Pathology, IRCCS, Rizzoli Orthopedic Institute, I‑40136 Bologna, 
      Italy.
FAU - Picci, Piero
AU  - Picci P
AD  - Laboratory of Experimental Oncology, IRCCS, Rizzoli Orthopedic Institute, I‑40136 
      Bologna, Italy.
FAU - Benassi, Maria Serena
AU  - Benassi MS
AD  - Laboratory of Experimental Oncology, IRCCS, Rizzoli Orthopedic Institute, I‑40136 
      Bologna, Italy.
LA  - eng
PT  - Journal Article
DEP - 20181106
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (CXCR4 protein, human)
RN  - 0 (MIRN494 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Receptors, CXCR4)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Disease Progression
MH  - Down-Regulation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Neoplasm Metastasis
MH  - Prognosis
MH  - Receptors, CXCR4/*genetics/*metabolism
MH  - Sarcoma, Synovial/*genetics/metabolism
MH  - Survival Analysis
MH  - Young Adult
OTO - NOTNLM
OT  - synovial sarcoma
OT  - microRNA-494.3p
OT  - C-X-C chemokine receptor 4
OT  - biomarker
OT  - cell migration
EDAT- 2018/11/16 06:00
MHDA- 2019/03/15 06:00
CRDT- 2018/11/16 06:00
PHST- 2018/05/29 00:00 [received]
PHST- 2018/10/04 00:00 [accepted]
PHST- 2018/11/16 06:00 [pubmed]
PHST- 2019/03/15 06:00 [medline]
PHST- 2018/11/16 06:00 [entrez]
AID - 10.3892/ijo.2018.4627 [doi]
PST - ppublish
SO  - Int J Oncol. 2019 Jan;54(1):361-369. doi: 10.3892/ijo.2018.4627. Epub 2018 Nov 6.