PMID- 30440003
OWN - NLM
STAT- MEDLINE
DCOM- 20190415
LR  - 20190415
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 11
DP  - 2018
TI  - Karyotype heterogeneity in Philodendron s.l. (Araceae) revealed by chromosome 
      mapping of rDNA loci.
PG  - e0207318
LID - 10.1371/journal.pone.0207318 [doi]
LID - e0207318
AB  - Philodendron s.l. (Araceae) has been recently focus of taxonomic and phylogenetic 
      studies, but karyotypic data are limited to chromosome numbers and a few 
      published genome sizes. In this work, karyotypes of 34 species of Philodendron 
      s.l. (29 species of Philodendron and five of Thaumatophyllum), ranging from 2n = 
      28 to 36 chromosomes, were analyzed by fluorescence in situ hybridization (FISH) 
      with rDNA and telomeric probes, aiming to understand the evolution of the 
      karyotype diversity of the group. Philodendron presented a high number variation 
      of 35S rDNA, ranging from two to 16 sites, which were mostly in the terminal 
      region of the short arms, with nine species presenting heteromorphisms. In the 
      case of Thaumatophyllum species, we observed a considerably lower variation, 
      which ranged from two to four terminal sites. The distribution of the 5S rDNA 
      clusters was more conserved, with two sites for most species, being preferably 
      located interstitially in the long chromosome arms. For the telomeric probe, 
      while exclusively terminal sites were observed for P. giganteum (2n = 30) 
      chromosomes, P. callosum (2n = 28) presented an interstitial distribution 
      associated with satellite DNA. rDNA sites of the analyzed species of Philodendron 
      s.l. species were randomly distributed considering the phylogenetic context, 
      probably due to rapid evolution and great diversity of these genomes. The 
      observed heteromorphisms suggest the accumulation of repetitive DNA in the 
      genomes of some species and the occurrence of chromosomal rearrangements along 
      the karyotype evolution of the group.
FAU - Vasconcelos, Emanuelle Varao
AU  - Vasconcelos EV
AUID- ORCID: 0000-0001-9429-5636
AD  - Departamento de Genetica, Centro de Biociencias, Universidade Federal de 
      Pernambuco, CEP, Recife, Pernambuco, Brazil.
FAU - Vasconcelos, Santelmo
AU  - Vasconcelos S
AD  - Instituto Tecnologico Vale, CEP, Belem, Para, Brazil.
FAU - Ribeiro, Tiago
AU  - Ribeiro T
AD  - Departamento de Botanica, Centro de Biociencias, Universidade Federal de 
      Pernambuco, CEP, Recife, Pernambuco, Brazil.
AD  - Instituto Federal de Mato Grosso, Campus Avancado de Diamantino, Rodovia Roberto 
      Campos, CEP, Diamantino, Mato Grosso, Brazil.
FAU - Benko-Iseppon, Ana Maria
AU  - Benko-Iseppon AM
AD  - Departamento de Genetica, Centro de Biociencias, Universidade Federal de 
      Pernambuco, CEP, Recife, Pernambuco, Brazil.
FAU - Brasileiro-Vidal, Ana Christina
AU  - Brasileiro-Vidal AC
AD  - Departamento de Genetica, Centro de Biociencias, Universidade Federal de 
      Pernambuco, CEP, Recife, Pernambuco, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181115
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - *Chromosome Mapping
MH  - Chromosomes, Plant/*genetics
MH  - DNA, Plant/*genetics
MH  - DNA, Ribosomal/*genetics
MH  - *Karyotype
MH  - Philodendron/*genetics
MH  - Species Specificity
PMC - PMC6237374
COIS- The authors have declared that no competing interests exist.
EDAT- 2018/11/16 06:00
MHDA- 2019/04/16 06:00
PMCR- 2018/11/15
CRDT- 2018/11/16 06:00
PHST- 2018/08/03 00:00 [received]
PHST- 2018/10/29 00:00 [accepted]
PHST- 2018/11/16 06:00 [entrez]
PHST- 2018/11/16 06:00 [pubmed]
PHST- 2019/04/16 06:00 [medline]
PHST- 2018/11/15 00:00 [pmc-release]
AID - PONE-D-18-22981 [pii]
AID - 10.1371/journal.pone.0207318 [doi]
PST - epublish
SO  - PLoS One. 2018 Nov 15;13(11):e0207318. doi: 10.1371/journal.pone.0207318. 
      eCollection 2018.