PMID- 30441770
OWN - NLM
STAT- MEDLINE
DCOM- 20190211
LR  - 20220716
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 19
IP  - 11
DP  - 2018 Nov 14
TI  - Transport of Apolipoprotein B-Containing Lipoproteins through Endothelial Cells 
      Is Associated with Apolipoprotein E-Carrying HDL-Like Particle Formation.
LID - 10.3390/ijms19113593 [doi]
LID - 3593
AB  - Passage of apolipoprotein B-containing lipoproteins (apoB-LPs), i.e., 
      triglyceride-rich lipoproteins (TRLs), intermediate-density lipoproteins (IDLs), 
      and low-density lipoproteins (LDLs), through the endothelial monolayer occurs in 
      normal and atherosclerotic arteries. Among these lipoproteins, TRLs and IDLs are 
      apoE-rich apoB-LPs (E/B-LPs). Recycling of TRL-associated apoE has been shown to 
      form apoE-carrying high-density lipoprotein (HDL)-like (HDL(E)) particles in many 
      types of cells. The current report studied the formation of HDL(E) particles by 
      transcytosis of apoB-LPs through mouse aortic endothelial cells (MAECs). Our data 
      indicated that passage of radiolabeled apoB-LPs, rich or poor in apoE, through 
      the MAEC monolayer is inhibited by filipin and unlabeled competitor lipoproteins, 
      suggesting that MAECs transport apoB-LPs via a caveolae-mediated pathway. The 
      cholesterol and apoE in the cell-untreated E/B-LPs, TRLs, IDLs, and LDLs 
      distributed primarily in the low-density (LD) fractions (d </= 1.063). A 
      substantial portion of the cholesterol and apoE that passed through the MAEC 
      monolayer was allotted into the high-density (HD) (d > 1.063) fractions. In 
      contrast, apoB was detectable only in the LD fractions before or after apoB-LPs 
      were incubated with the MAEC monolayer, suggesting that apoB-LPs pass through the 
      MAEC monolayer in the forms of apoB-containing LD particles and apoE-containing 
      HD particles.
FAU - Yang, Hong
AU  - Yang H
AD  - Department of Microbiology, Immunology and Physiology, Meharry Medical College, 
      Nashville, TN 37208, USA. hyang@mmc.edu.
FAU - Zhang, Ningya
AU  - Zhang N
AD  - Department of Microbiology, Immunology and Physiology, Meharry Medical College, 
      Nashville, TN 37208, USA. nzhang@mmc.edu.
FAU - Okoro, Emmanuel U
AU  - Okoro EU
AD  - Department of Microbiology, Immunology and Physiology, Meharry Medical College, 
      Nashville, TN 37208, USA. eokoro@mmc.edu.
FAU - Guo, Zhongmao
AU  - Guo Z
AD  - Department of Microbiology, Immunology and Physiology, Meharry Medical College, 
      Nashville, TN 37208, USA. zguo@mmc.edu.
LA  - eng
GR  - SC1 HL101431/HL/NHLBI NIH HHS/United States
GR  - SC1HL101431/NH/NIH HHS/United States
PT  - Journal Article
DEP - 20181114
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Apolipoproteins B)
RN  - 0 (Apolipoproteins E)
RN  - 0 (Lipoproteins, HDL)
RN  - 0 (Lipoproteins, LDL)
SB  - IM
MH  - Animals
MH  - Apolipoproteins B/*metabolism
MH  - Apolipoproteins E/*metabolism
MH  - Cells, Cultured
MH  - Endothelial Cells/*metabolism
MH  - Endothelium, Vascular/cytology/*metabolism
MH  - Lipoproteins, HDL/metabolism
MH  - Lipoproteins, LDL/metabolism
MH  - Mice
MH  - *Transcytosis
PMC - PMC6274886
OTO - NOTNLM
OT  - apolipoprotein B-containing lipoprotein
OT  - apolipoprotein E
OT  - high-density lipoprotein formation
OT  - mouse aortic endothelial cells
OT  - transendothelial transport
COIS- The authors declare no conflicts of interest.
EDAT- 2018/11/18 06:00
MHDA- 2019/02/12 06:00
PMCR- 2018/11/01
CRDT- 2018/11/17 06:00
PHST- 2018/10/24 00:00 [received]
PHST- 2018/11/09 00:00 [revised]
PHST- 2018/11/11 00:00 [accepted]
PHST- 2018/11/17 06:00 [entrez]
PHST- 2018/11/18 06:00 [pubmed]
PHST- 2019/02/12 06:00 [medline]
PHST- 2018/11/01 00:00 [pmc-release]
AID - ijms19113593 [pii]
AID - ijms-19-03593 [pii]
AID - 10.3390/ijms19113593 [doi]
PST - epublish
SO  - Int J Mol Sci. 2018 Nov 14;19(11):3593. doi: 10.3390/ijms19113593.