PMID- 30443710
OWN - NLM
STAT- MEDLINE
DCOM- 20190515
LR  - 20200225
IS  - 1476-3524 (Electronic)
IS  - 1029-8428 (Linking)
VI  - 35
IP  - 3
DP  - 2019 Apr
TI  - Postnatal Administration of Homocysteine Induces Cerebellar Damage in Rats: 
      Protective Effect of Folic Acid.
PG  - 724-738
LID - 10.1007/s12640-018-9979-y [doi]
AB  - A widely held view suggests that homocysteine (Hcy) can contribute to 
      neurodegeneration through promotion of oxidative stress. There is evidence that 
      homocysteine is toxic to cerebellar Purkinje neurons in vitro; however, in vivo 
      action of Hcy on Purkinje cell has not been investigated so far. Thus, this study 
      was designed to evaluate the Hcy effects on neonatal rat cerebellum and 
      cerebellar oxidative stress. We also evaluated the folic acid effects on 
      biochemical alterations elicited by hyperhomocysteinemia (hHcy) in the 
      cerebellum. Group I received normal saline, group II received Hcy subcutaneously 
      twice a day at 8-h intervals (0.3-0.6 mumol/g body weight), group III received 
      Hcy + folic acid (0.011 mumol/g body weight), and group IV received folic acid on 
      postnatal day (PD) 4 until 25. On day 25, superoxide dismutase (SOD) and 
      glutathione peroxidase (GPx) activities in the cerebellum and motor cortex were 
      assayed. Malondialdehyde (MDA) levels were also evaluated as a marker of lipid 
      peroxidation. Rotarod and locomotor activity tests were performed in PD 25-27. 
      Our results indicated that administration of Hcy increased plasma, cortical, and 
      cerebellar total Hcy levels; reduced GPx activity; and induced lipid peroxidation 
      in the cerebellum. Hcy impaired performance on the rotarod in rats. However, 
      treatment with folic acid significantly attenuated motor coordination impairment, 
      GPx activity reduction, the lipid peroxidation process, and significantly reduced 
      plasma total Hcy levels. Histological analysis indicated that Hcy could decrease 
      Purkinje cell count and folic acid prevented this toxic effect. We conclude that 
      Hcy can induce neurotoxicity and folic acid has neuroprotective effects against 
      cerebellar Hcy toxicity.
FAU - Koohpeyma, Hakimeh
AU  - Koohpeyma H
AD  - School of Biology, Damghan University, Damghan, 3671641167, Iran.
FAU - Goudarzi, Iran
AU  - Goudarzi I
AUID- ORCID: 0000-0002-6665-3202
AD  - School of Biology, Damghan University, Damghan, 3671641167, Iran. 
      irangoudarzi@du.ac.ir.
FAU - Elahdadi Salmani, Mahmoud
AU  - Elahdadi Salmani M
AD  - School of Biology, Damghan University, Damghan, 3671641167, Iran.
FAU - Lashkarbolouki, Taghi
AU  - Lashkarbolouki T
AD  - School of Biology, Damghan University, Damghan, 3671641167, Iran.
FAU - Shabani, Mohammad
AU  - Shabani M
AD  - Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, 
      Iran.
LA  - eng
PT  - Journal Article
DEP - 20181115
PL  - United States
TA  - Neurotox Res
JT  - Neurotoxicity research
JID - 100929017
RN  - 0 (Antioxidants)
RN  - 0 (Neuroprotective Agents)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 935E97BOY8 (Folic Acid)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Cerebellar Diseases/chemically induced/drug therapy/metabolism/pathology
MH  - Cerebellum/*drug effects/*growth & development/metabolism/pathology
MH  - Folic Acid/blood/*pharmacology
MH  - Homocysteine/*adverse effects/blood
MH  - Lipid Peroxidation/drug effects/physiology
MH  - Male
MH  - Motor Cortex/drug effects/growth & development/metabolism/pathology
MH  - Neurodegenerative Diseases/chemically induced/drug therapy/metabolism/pathology
MH  - Neuroprotective Agents/blood/*pharmacology
MH  - Oxidative Stress/drug effects/physiology
MH  - Random Allocation
MH  - Rats, Wistar
OTO - NOTNLM
OT  - Folic acid
OT  - Homocysteine
OT  - Oxidative stress
OT  - Purkinje cell
OT  - Rat
EDAT- 2018/11/18 06:00
MHDA- 2019/05/16 06:00
CRDT- 2018/11/17 06:00
PHST- 2018/08/08 00:00 [received]
PHST- 2018/11/07 00:00 [accepted]
PHST- 2018/10/21 00:00 [revised]
PHST- 2018/11/18 06:00 [pubmed]
PHST- 2019/05/16 06:00 [medline]
PHST- 2018/11/17 06:00 [entrez]
AID - 10.1007/s12640-018-9979-y [pii]
AID - 10.1007/s12640-018-9979-y [doi]
PST - ppublish
SO  - Neurotox Res. 2019 Apr;35(3):724-738. doi: 10.1007/s12640-018-9979-y. Epub 2018 
      Nov 15.