PMID- 30445242
OWN - NLM
STAT- MEDLINE
DCOM- 20190208
LR  - 20190215
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 169
DP  - 2019 Mar
TI  - Analysis of glycerophospholipid metabolism after exposure to PCB153 in PC12 cells 
      through targeted lipidomics by UHPLC-MS/MS.
PG  - 120-127
LID - S0147-6513(18)31143-6 [pii]
LID - 10.1016/j.ecoenv.2018.11.006 [doi]
AB  - Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) that 
      have neurotoxicity, reproductive toxicity, hepatotoxicity and immunotoxicity in 
      both animals and humans. Few studies have focused on the changes to endogenous 
      glycerophospholipid metabolism caused by PCB153. To evaluate the relationships 
      between exposure to PCB153 and specific endogenous glycerophospholipid 
      metabolism, an ultra-high-performance liquid chromatography tandem mass 
      spectrometry (UHPLC-MS/MS) method was implemented in this study. Twenty-two 
      endogenous glycerophospholipids in PC12 cells were analyzed after exposure to 
      PCB153 at dosages of 0.05 mug mL(-1), 0.5 mug mL(-1) or 20 mug mL(-1) for 120 h. 
      PC(14:0/14:0), PE(16:0/18:1), PE(16:0/18:2), PS(18:0/18:1) and PI(16:0/18:1) were 
      identified as potential biomarkers under the rules of t-test (P) value < 0.05 and 
      variable importance at projection (VIP) value > 1. It was also found that the 
      alterations at 0.05 mug mL(-1) and 20 mug mL(-1) PCB153 were similar at 120 h, 
      while 0.5 mug mL(-1) PCB153 presented an opposite trend. Additionally, significant 
      upregulation of PC, PE and PS with the same fatty acid chains of 18:0/18:2 was 
      found after exposure to 0.05 mug mL(-1) and 20 mug mL(-1) PCB153 at 120 h. This 
      study revealed that PCB153 exposure modulated 22 endogenous glycerophospholipids 
      in PC12 cells and provided the basis for the further study of PCB153 on the 
      effects of glycerophospholipids on PC12 cells.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Wang, Xinlu
AU  - Wang X
AD  - Institute of Quality Standards and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, 
      Ministry of Agriculture, Beijing 100081, China.
FAU - Xu, Yanyang
AU  - Xu Y
AD  - Institute of Quality Standards and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, 
      Ministry of Agriculture, Beijing 100081, China.
FAU - Song, Xiao
AU  - Song X
AD  - Institute of Quality Standards and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, 
      Ministry of Agriculture, Beijing 100081, China.
FAU - Jia, Qi
AU  - Jia Q
AD  - Institute of Quality Standards and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, 
      Ministry of Agriculture, Beijing 100081, China.
FAU - Zhang, Xining
AU  - Zhang X
AD  - Institute of Quality Standards and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, 
      Ministry of Agriculture, Beijing 100081, China.
FAU - Qian, Yongzhong
AU  - Qian Y
AD  - Institute of Quality Standards and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, 
      Ministry of Agriculture, Beijing 100081, China. Electronic address: 
      qianyongzhong@caas.cn.
FAU - Qiu, Jing
AU  - Qiu J
AD  - Institute of Quality Standards and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, 
      Ministry of Agriculture, Beijing 100081, China. Electronic address: 
      qiujing@caas.cn.
LA  - eng
PT  - Journal Article
DEP - 20181113
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Environmental Pollutants)
RN  - 0 (Fatty Acids)
RN  - 0 (Glycerophospholipids)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - ZRU0C9E32O (2,4,5,2',4',5'-hexachlorobiphenyl)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Dose-Response Relationship, Drug
MH  - Environmental Pollutants/*toxicity
MH  - Fatty Acids/*biosynthesis
MH  - Glycerophospholipids/*metabolism
MH  - Humans
MH  - PC12 Cells
MH  - Polychlorinated Biphenyls/*toxicity
MH  - Rats
MH  - Tandem Mass Spectrometry
MH  - Up-Regulation
OTO - NOTNLM
OT  - Glycerophospholipid metabolism
OT  - Lipidomics
OT  - Neurotoxicity
OT  - PC12 cell
OT  - PCB153
EDAT- 2018/11/18 06:00
MHDA- 2019/02/09 06:00
CRDT- 2018/11/17 06:00
PHST- 2018/07/02 00:00 [received]
PHST- 2018/10/01 00:00 [revised]
PHST- 2018/11/01 00:00 [accepted]
PHST- 2018/11/18 06:00 [pubmed]
PHST- 2019/02/09 06:00 [medline]
PHST- 2018/11/17 06:00 [entrez]
AID - S0147-6513(18)31143-6 [pii]
AID - 10.1016/j.ecoenv.2018.11.006 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2019 Mar;169:120-127. doi: 10.1016/j.ecoenv.2018.11.006. 
      Epub 2018 Nov 13.