PMID- 30445554
OWN - NLM
STAT- MEDLINE
DCOM- 20190430
LR  - 20190430
IS  - 1758-4469 (Electronic)
IS  - 0829-318X (Linking)
VI  - 39
IP  - 3
DP  - 2019 Mar 1
TI  - Molecular regulatory mechanism of isoprene emission under short-term drought 
      stress in the tropical tree Ficus septica.
PG  - 440-453
LID - 10.1093/treephys/tpy123 [doi]
AB  - Isoprene is emitted by many plants and is thought to function as an antioxidant 
      under stressful conditions. However, the detailed regulatory mechanism of 
      isoprene emission in relation to the antioxidant system remains unclear. 
      Therefore, in this study, we explored the molecular regulatory mechanism of 
      isoprene emission under short-term drought stress in the tropical tree Ficus 
      septica Burm.f. We found that the soil moisture content gradually decreased from 
      55% on Day 1 (D1) to 23% (wilting point) on D5 after withholding water for 4 days 
      and then returning to the initial level following re-watering on D6. On D5, 
      drought-stressed plants had more than twofold higher isoprene emission and 90.6% 
      lower photosynthesis rates, 99.5% lower stomatal conductance and 82.3% lower 
      transpiration rates than well-watered control plants. It was also estimated that 
      the isoprene concentration inside the leaf greatly increased on D5 due to the 
      increased isoprene emission rate and reduced stomatal conductance. Among the 
      traits related to the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway, which is 
      responsible for isoprene biosynthesis, the isoprene synthase (IspS) protein level 
      was positively correlated with the isoprene emission rate in stressed plants. The 
      transcripts of the antioxidant genes peroxidase 2 (POD2), POD4, copper-zinc 
      superoxide dismutase 2 (Cu-ZnSOD2) and manganese superoxide dismutase 1 (Mn-SOD1) 
      also increased during the drying period, while those of ascorbate peroxidase 1 
      (APX1) decreased. However, there was only a weak correlation between isoprene 
      emission and antioxidant enzyme gene expression, indicating that the regulation 
      of isoprene biosynthesis is not directly linked to the antioxidant defense 
      network in drought-stressed F. septica. These findings suggest that the 
      post-transcriptional regulation of IspS led to the observed change in isoprene 
      emission rate, which enhanced the quenching of reactive oxygen species (ROS) and, 
      in combination with the increased antioxidant enzyme activity, conferred 
      tolerance to drought stress in this species.
CI  - (c) The Author(s) 2018. Published by Oxford University Press. All rights reserved. 
      For Permissions, please email: journals.permissions@oup.com.
FAU - Parveen, Shahanaz
AU  - Parveen S
AD  - Tropical Biosphere Research Center, University of the Ryukyus, Senbaru 1, 
      Nishihara, Okinawa, Japan.
AD  - The United Graduate School of Agricultural Sciences, Kagoshima University, 
      Korimoto 1-21-24, Kagoshima, Japan.
AD  - Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla 
      Nagar, Dhaka, Bangladesh.
FAU - Rashid, Md Harun-Ur-
AU  - Rashid MH
AD  - The United Graduate School of Agricultural Sciences, Kagoshima University, 
      Korimoto 1-21-24, Kagoshima, Japan.
AD  - Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla 
      Nagar, Dhaka, Bangladesh.
FAU - Inafuku, Masashi
AU  - Inafuku M
AD  - Tropical Biosphere Research Center, University of the Ryukyus, Senbaru 1, 
      Nishihara, Okinawa, Japan.
FAU - Iwasaki, Hironori
AU  - Iwasaki H
AD  - Tropical Biosphere Research Center, University of the Ryukyus, Senbaru 1, 
      Nishihara, Okinawa, Japan.
FAU - Oku, Hirosuke
AU  - Oku H
AD  - Tropical Biosphere Research Center, University of the Ryukyus, Senbaru 1, 
      Nishihara, Okinawa, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Tree Physiol
JT  - Tree physiology
JID - 100955338
RN  - 0 (Antioxidants)
RN  - 0 (Butadienes)
RN  - 0 (Hemiterpenes)
RN  - 0 (Plant Proteins)
RN  - 0A62964IBU (isoprene)
SB  - IM
MH  - Antioxidants/*physiology
MH  - Butadienes/*metabolism
MH  - *Droughts
MH  - Ficus/*physiology
MH  - Hemiterpenes/*metabolism
MH  - Plant Proteins/metabolism
MH  - Stress, Physiological
MH  - Transcription, Genetic/physiology
MH  - Trees/physiology
OTO - NOTNLM
OT  - Ficus septica
OT  - IspS protein
OT  - MEP pathway
OT  - ROS
OT  - drought
OT  - isoprene
EDAT- 2018/11/18 06:00
MHDA- 2019/05/01 06:00
CRDT- 2018/11/17 06:00
PHST- 2018/04/06 00:00 [received]
PHST- 2018/09/04 00:00 [revised]
PHST- 2018/10/08 00:00 [accepted]
PHST- 2018/11/18 06:00 [pubmed]
PHST- 2019/05/01 06:00 [medline]
PHST- 2018/11/17 06:00 [entrez]
AID - 5184640 [pii]
AID - 10.1093/treephys/tpy123 [doi]
PST - ppublish
SO  - Tree Physiol. 2019 Mar 1;39(3):440-453. doi: 10.1093/treephys/tpy123.