PMID- 30445673
OWN - NLM
STAT- MEDLINE
DCOM- 20190218
LR  - 20231004
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 19
IP  - 11
DP  - 2018 Nov 15
TI  - Design of a 3D BMP-2-Delivering Tannylated PCL Scaffold and Its Anti-Oxidant, 
      Anti-Inflammatory, and Osteogenic Effects In Vitro.
LID - 10.3390/ijms19113602 [doi]
LID - 3602
AB  - In this study, a novel three-dimensional (3D) bone morphogenic protein-2 
      (BMP-2)-delivering tannylated polycaprolactone (PCL) (BMP-2/tannic acid (TA)/PCL) 
      scaffold with anti-oxidant, anti-inflammatory, and osteogenic activities was 
      fabricated via simple surface coating with TA, followed by the immobilization of 
      BMP-2 on the TA-coated PCL scaffold. The BMP-2/TA/PCL scaffold showed controlled 
      and sustained BMP-2 release. It effectively scavenged reactive oxygen species 
      (ROS) in cells, and increased the proliferation of MC3T3-E1 cells pre-treated 
      with hydrogen peroxide (H(2)O(2)). Additionally, the BMP-2/TA/PCL scaffold 
      significantly suppressed the mRNA levels of pro-inflammatory cytokines, including 
      matrix metalloproteinases-3 (MMP-3), cyclooxygenase-2 (COX-2), interleukin-6 
      (IL-6), and tumor necrosis factor-alpha (TNF-alpha), in lipopolysaccharide (LPS)-induced 
      MC3T3-E1 cells. Furthermore, it showed outstanding enhancement of the osteogenic 
      activity of MC3T3-E1 cells through increased alkaline phosphatase (ALP) activity 
      and calcium deposition. Our findings demonstrated that the BMP-2/TA/PCL scaffold 
      plays an important role in scavenging ROS, suppressing inflammatory response, and 
      enhancing the osteogenic differentiation of cells.
FAU - Lee, Jae Yong
AU  - Lee JY
AD  - Department of Orthopedic Surgery and Rare Diseases Institute, Korea University 
      Guro Hospital, Korea University College of Medicine, 148 Guro-dong, Guro-gu, 
      Seoul 08308, Korea. bulegir@nate.com.
FAU - Lim, Hyunwoong
AU  - Lim H
AD  - Department of Industrial Management Engineering, Korea University, 145 Anam-ro, 
      Seongbuk-gu, Seoul 02841, Korea. limhw@korea.ac.kr.
FAU - Ahn, Jae Won
AU  - Ahn JW
AD  - Department of Systems Biotechnology, Chung-Ang University, Anseong, Gyeonggi-do 
      17546, Korea. pinkymonkey1@naver.com.
FAU - Jang, Dongsik
AU  - Jang D
AD  - Department of Industrial Management Engineering, Korea University, 145 Anam-ro, 
      Seongbuk-gu, Seoul 02841, Korea. Jang@korea.ac.kr.
FAU - Lee, Seung Hee
AU  - Lee SH
AD  - Department of Nursing, Inha University, 100 Inha-ro, Michuhol-gu, Incheon 22212, 
      Korea. freelovein@hanmail.net.
FAU - Park, Kyeongsoon
AU  - Park K
AUID- ORCID: 0000-0003-3625-4128
AD  - Department of Systems Biotechnology, Chung-Ang University, Anseong, Gyeonggi-do 
      17546, Korea. kspark1223@cau.ac.kr.
FAU - Kim, Sung Eun
AU  - Kim SE
AD  - Department of Orthopedic Surgery and Rare Diseases Institute, Korea University 
      Guro Hospital, Korea University College of Medicine, 148 Guro-dong, Guro-gu, 
      Seoul 08308, Korea. sekim10@korea.ac.kr.
LA  - eng
GR  - 2017/Chung-Ang University Research Scholarship Grants/
PT  - Journal Article
DEP - 20181115
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Polyesters)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tannins)
RN  - 24980-41-4 (polycaprolactone)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Antioxidants/*pharmacology
MH  - Bone Morphogenetic Protein 2/*pharmacology
MH  - Calcium/metabolism
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Drug Liberation
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - Osteogenesis/*drug effects
MH  - Photoelectron Spectroscopy
MH  - Polyesters/*chemistry
MH  - RNA, Messenger/genetics/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Tannins/*chemistry
MH  - Tissue Scaffolds/*chemistry
PMC - PMC6274927
OTO - NOTNLM
OT  - 3D scaffold
OT  - anti-inflammation
OT  - anti-oxidation
OT  - bone morphogenic protein-2 (BMP-2)
OT  - osteogenic differentiation
OT  - polycaprolactone (PCL)
OT  - tannic acid (TA)
COIS- The authors have no conflicts of interest to declare.
EDAT- 2018/11/18 06:00
MHDA- 2019/02/20 06:00
PMCR- 2018/11/01
CRDT- 2018/11/18 06:00
PHST- 2018/10/10 00:00 [received]
PHST- 2018/11/05 00:00 [revised]
PHST- 2018/11/12 00:00 [accepted]
PHST- 2018/11/18 06:00 [entrez]
PHST- 2018/11/18 06:00 [pubmed]
PHST- 2019/02/20 06:00 [medline]
PHST- 2018/11/01 00:00 [pmc-release]
AID - ijms19113602 [pii]
AID - ijms-19-03602 [pii]
AID - 10.3390/ijms19113602 [doi]
PST - epublish
SO  - Int J Mol Sci. 2018 Nov 15;19(11):3602. doi: 10.3390/ijms19113602.