PMID- 30446138
OWN - NLM
STAT- MEDLINE
DCOM- 20181219
LR  - 20181219
IS  - 1879-1344 (Electronic)
IS  - 0144-8617 (Linking)
VI  - 205
DP  - 2019 Feb 1
TI  - Mechanism of Lycium barbarum polysaccharides liposomes on activating murine 
      dendritic cells.
PG  - 540-549
LID - S0144-8617(18)31246-3 [pii]
LID - 10.1016/j.carbpol.2018.10.057 [doi]
AB  - Dendritic cells (DCs) are professional antigen-presenting cells (APC) that play a 
      central role in the initiation and regulation of immune responses. We have 
      previously demonstrated that Lycium barbarum polysaccharides liposomes (LBPL) as 
      immune adjuvant elicits strong antigen-specific Th1 immune responses. The purpose 
      of this study was to investigate underlying mechanism of liposomes promoting 
      effect of Lycium barbarum polysaccharides (LBP) on activating DCs. LBP were 
      loaded with high entrapment efficiency (86%) into liposomes using reverse phase 
      evaporation. LBPL activation of phenotypic and functional maturation of DCs was 
      explored through mechanistic studies of the TLR4-MyD88-NF-kappaB signaling pathway 
      and amount of proinflammatory cytokines released. We found that LBPL indeed 
      activated immature DCs and induced DCs maturation characterized by up-regulation 
      of co-stimulatory molecules (MHCII, CD80, CD86), production of cytokines 
      (IL-12p40, TNF-alpha), and enhancement of antigen uptake. Additionally, we 
      demonstrated that liposomes could promote LBP up-regulation of TLR4, MyD88, 
      TRAF6, NF-kappaB gene and protein expression.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Bo, Ruonan
AU  - Bo R
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
FAU - Liu, Zhenguang
AU  - Liu Z
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Wuhan HVSEN Biotechnology Co., Ltd., Zhangbai Road, Dongxihu District, Wuhan, 
      430042, PR China.
FAU - Gu, Pengfei
AU  - Gu P
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
FAU - Ou, Ning
AU  - Ou N
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
FAU - Sun, Yaqin
AU  - Sun Y
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
FAU - Hu, Yuanliang
AU  - Hu Y
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
FAU - Liu, Jiaguo
AU  - Liu J
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
FAU - Wang, Deyun
AU  - Wang D
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic 
      address: dywang@njau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20181025
PL  - England
TA  - Carbohydr Polym
JT  - Carbohydrate polymers
JID - 8307156
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Interleukin-12 Subunit p40)
RN  - 0 (Liposomes)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NF-kappa B)
RN  - 0 (Polysaccharides)
RN  - 0 (TNF Receptor-Associated Factor 6)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (lycium barbarum polysaccharide)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/metabolism
MH  - Cell Proliferation/drug effects
MH  - Dendritic Cells/*drug effects/metabolism
MH  - Drugs, Chinese Herbal/chemistry/*pharmacology
MH  - Female
MH  - Interleukin-12 Subunit p40/metabolism
MH  - Liposomes/chemistry/*pharmacology
MH  - Lycium/*chemistry
MH  - Mice, Inbred C57BL
MH  - Myeloid Differentiation Factor 88/genetics/metabolism
MH  - NF-kappa B/genetics/metabolism
MH  - Polysaccharides/chemistry/*pharmacology
MH  - Signal Transduction/drug effects
MH  - Spectroscopy, Fourier Transform Infrared
MH  - TNF Receptor-Associated Factor 6/genetics/metabolism
MH  - Toll-Like Receptor 4/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - Antigen uptake
OT  - Dendritic cells
OT  - Lycium barbarum polysaccharides liposomes
OT  - Phenotypic and functional maturation
OT  - Proinflammatory cytokines
OT  - TLR4-MyD88-NF-kappaB signaling pathway
EDAT- 2018/11/18 06:00
MHDA- 2018/12/20 06:00
CRDT- 2018/11/18 06:00
PHST- 2018/08/08 00:00 [received]
PHST- 2018/09/25 00:00 [revised]
PHST- 2018/10/19 00:00 [accepted]
PHST- 2018/11/18 06:00 [entrez]
PHST- 2018/11/18 06:00 [pubmed]
PHST- 2018/12/20 06:00 [medline]
AID - S0144-8617(18)31246-3 [pii]
AID - 10.1016/j.carbpol.2018.10.057 [doi]
PST - ppublish
SO  - Carbohydr Polym. 2019 Feb 1;205:540-549. doi: 10.1016/j.carbpol.2018.10.057. Epub 
      2018 Oct 25.