PMID- 30448222
OWN - NLM
STAT- MEDLINE
DCOM- 20191125
LR  - 20191125
IS  - 1532-8392 (Electronic)
IS  - 0046-8177 (Linking)
VI  - 85
DP  - 2019 Mar
TI  - Immunohistochemical pattern of c-MYC protein judged as "+/(weak)+/-" by a new 
      notation correlates with MYC gene nontranslocation in large B-cell lymphoma.
PG  - 112-118
LID - S0046-8177(18)30422-2 [pii]
LID - 10.1016/j.humpath.2018.10.025 [doi]
AB  - Immunohistochemistry is not only the most important tool for pathologists to 
      establish a final diagnosis, but it can also inform decisions regarding optimal 
      treatment methods. However, there is no universal standard notation for 
      expressing immunohistochemical findings. For a diagnosis of malignant lymphoma, 
      it is important to confirm the presence or absence of MYC translocation and 
      communicate these results to a clinical audience. However, the criteria for 
      selecting cases for fluorescence in situ hybridization (FISH) analysis to confirm 
      MYC translocation are ill defined. We therefore devised a notation that we termed 
      proportion of immunoreactivity/expression for immunohistochemistry (PRIME 
      notation) based on the cellular proportion showing different antigen-antibody 
      reactivity in immunohistochemistry (CPAR) and used it to examine the relationship 
      between MYC translocation and the proportion of c-MYC+ lymphoma cells. We 
      reviewed 82 cases diagnosed as diffuse large B-cell lymphoma or diffuse large 
      B-cell lymphoma coexisting with grade 3A to 3B follicular lymphoma. The most 
      common notation was "+/(weak)+/-" (49/82 cases [59.8%]); cases that were CPAR 
      positive, weakly positive, and negative for tumor cells each accounted for about 
      one-third of the total. Unexpectedly, no MYC translocation was observed by FISH 
      in this group. Thus, FISH is not needed even if more than half of cells are c-MYC 
      positive by PRIME notation. This is the first report describing a correspondence 
      between immunohistochemical findings and chromosomal abnormality, reflecting 
      findings at the protein and gene levels, respectively.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Fujishima, Fumiyoshi
AU  - Fujishima F
AD  - Department of Anatomic Pathology, Tohoku University Graduate School of Medicine, 
      Aoba-ku, Sendai 980-8574, Japan. Electronic address: 
      ffujishima@patholo2.med.tohoku.ac.jp.
FAU - Katsushima, Hiroki
AU  - Katsushima H
AD  - Division of Hematopathology, Tohoku University Hospital, Aoba-ku, Sendai 
      980-8574, Japan.
FAU - Fukuhara, Noriko
AU  - Fukuhara N
AD  - Department of Hematology and Rheumatology, Tohoku University Graduate School of 
      Medicine, Aoba-ku, Sendai 980-8574, Japan.
FAU - Konosu-Fukaya, Sachiko
AU  - Konosu-Fukaya S
AD  - Division of Pathology, Tohoku Medical and Pharmaceutical University, Miyagino-ku, 
      Sendai 983-8536, Japan.
FAU - Nakamura, Yasuhiro
AU  - Nakamura Y
AD  - Division of Pathology, Tohoku Medical and Pharmaceutical University, Miyagino-ku, 
      Sendai 983-8536, Japan.
FAU - Usubuchi, Hajime
AU  - Usubuchi H
AD  - Department of Pathology, Tohoku University Hospital, Aoba-ku, Sendai 980-8574, 
      Japan.
FAU - Sato, Satoko
AU  - Sato S
AD  - Department of Pathology, Tohoku University Hospital, Aoba-ku, Sendai 980-8574, 
      Japan.
FAU - Ota, Yasunori
AU  - Ota Y
AD  - Depaertment of Pathology, The Institute of Medical Science, The University of 
      Tokyo, Minato-ku, Tokyo 108-8639, Japan.
FAU - Yashima-Abo, Akiko
AU  - Yashima-Abo A
AD  - Department of Pathology, School of Medicine, Iwate Medical University, Morioka, 
      Iwate 020-0023, Japan.
FAU - Nakamura, Tomohiro
AU  - Nakamura T
AD  - Department of Preventive Medicine and Epidemiology, Tohoku Medical Megabank 
      Organization, Tohoku University, Aoba-ku, Sendai 980-8574, Japan.
FAU - Nakaya, Naoki
AU  - Nakaya N
AD  - Department of Preventive Medicine and Epidemiology, Tohoku Medical Megabank 
      Organization, Tohoku University, Aoba-ku, Sendai 980-8574, Japan.
FAU - Harigae, Hideo
AU  - Harigae H
AD  - Department of Hematology and Rheumatology, Tohoku University Graduate School of 
      Medicine, Aoba-ku, Sendai 980-8574, Japan.
FAU - Sasano, Hironobu
AU  - Sasano H
AD  - Department of Anatomic Pathology, Tohoku University Graduate School of Medicine, 
      Aoba-ku, Sendai 980-8574, Japan; Department of Pathology, Tohoku University 
      Hospital, Aoba-ku, Sendai 980-8574, Japan.
FAU - Ichinohasama, Ryo
AU  - Ichinohasama R
AD  - Division of Hematopathology, Tohoku University Hospital, Aoba-ku, Sendai 
      980-8574, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181115
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MYC protein, human)
RN  - 0 (Proto-Oncogene Proteins c-myc)
SB  - IM
MH  - Biomarkers, Tumor/metabolism
MH  - *Gene Rearrangement
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, Follicular/genetics/*metabolism/pathology
MH  - Lymphoma, Large B-Cell, Diffuse/genetics/*metabolism/pathology
MH  - Proto-Oncogene Proteins c-myc/genetics/*metabolism
OTO - NOTNLM
OT  - CPAR
OT  - FISH
OT  - Immunohistochemistry
OT  - MYC
OT  - PRIME notation
EDAT- 2018/11/19 06:00
MHDA- 2019/11/26 06:00
CRDT- 2018/11/19 06:00
PHST- 2018/07/14 00:00 [received]
PHST- 2018/10/30 00:00 [revised]
PHST- 2018/10/31 00:00 [accepted]
PHST- 2018/11/19 06:00 [pubmed]
PHST- 2019/11/26 06:00 [medline]
PHST- 2018/11/19 06:00 [entrez]
AID - S0046-8177(18)30422-2 [pii]
AID - 10.1016/j.humpath.2018.10.025 [doi]
PST - ppublish
SO  - Hum Pathol. 2019 Mar;85:112-118. doi: 10.1016/j.humpath.2018.10.025. Epub 2018 
      Nov 15.