PMID- 30448381
OWN - NLM
STAT- MEDLINE
DCOM- 20190219
LR  - 20200309
IS  - 1095-6840 (Electronic)
IS  - 0016-6480 (Print)
IS  - 0016-6480 (Linking)
VI  - 272
DP  - 2019 Feb 1
TI  - Effects of thyroid hormone disruption on the ontogenetic expression of thyroid 
      hormone signaling genes in developing zebrafish (Danio rerio).
PG  - 20-32
LID - S0016-6480(18)30305-8 [pii]
LID - 10.1016/j.ygcen.2018.11.007 [doi]
AB  - Thyroid hormones (THs) regulate neurodevelopment, thus TH disruption is widely 
      posited as a mechanism of developmental neurotoxicity for diverse environmental 
      chemicals. Zebrafish have been proposed as an alternative model for studying the 
      role of TH in developmental neurotoxicity. To realize this goal, it is critical 
      to characterize the normal ontogenetic expression profile of TH signaling 
      molecules in the developing zebrafish and determine the sensitivity of these 
      molecules to perturbations in TH levels. To address these gaps in the existing 
      database, we characterized the transcriptional profiles of TH transporters, 
      deiodinases (DIOs), receptors (TRs), nuclear coactivators (NCOAs), nuclear 
      corepressors (NCORs), and retinoid X receptors (RXRs) in parallel with 
      measurements of endogenous TH concentrations and tshbeta mRNA expression throughout 
      the first five days of zebrafish development. Transcripts encoding these TH 
      signaling components were identified and observed to be upregulated around 
      48-72 h post fertilization (hpf) concurrent with the onset of larval production 
      of T4. Exposure to exogenous T4 and T3 upregulated mct8, dio3-b, tralpha-a, trbeta, and 
      mbp-a levels, and downregulated expression of oatp1c1. Morpholino knockdown of TH 
      transporter mct8 and treatment with 6-propyl-2-thiouracil (PTU) was used to 
      reduce cellular uptake and production of TH, an effect that was associated with 
      downregulation of dio3-b at 120 hpf. Collectively, these data confirm that larval 
      zebrafish express orthologs of TH signaling molecules important in mammalian 
      development and suggest that there may be species differences with respect to 
      impacts of TH disruption on gene transcription.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Walter, Kyla M
AU  - Walter KM
AD  - Department of Molecular Biosciences, University of California-Davis School of 
      Veterinary Medicine, Davis, CA 95616, United States. Electronic address: 
      kwalter@ucdavis.edu.
FAU - Miller, Galen W
AU  - Miller GW
AD  - Department of Molecular Biosciences, University of California-Davis School of 
      Veterinary Medicine, Davis, CA 95616, United States. Electronic address: 
      gwmiller@ucdavis.edu.
FAU - Chen, Xiaopeng
AU  - Chen X
AD  - Department of Molecular Biosciences, University of California-Davis School of 
      Veterinary Medicine, Davis, CA 95616, United States. Electronic address: 
      chenxiaopengemail@gmail.com.
FAU - Yaghoobi, Bianca
AU  - Yaghoobi B
AD  - Department of Molecular Biosciences, University of California-Davis School of 
      Veterinary Medicine, Davis, CA 95616, United States. Electronic address: 
      byaghoobi@ucdavis.edu.
FAU - Puschner, Birgit
AU  - Puschner B
AD  - Department of Molecular Biosciences, University of California-Davis School of 
      Veterinary Medicine, Davis, CA 95616, United States. Electronic address: 
      bpuschner@ucdavis.edu.
FAU - Lein, Pamela J
AU  - Lein PJ
AD  - Department of Molecular Biosciences, University of California-Davis School of 
      Veterinary Medicine, Davis, CA 95616, United States. Electronic address: 
      pjlein@ucdavis.edu.
LA  - eng
GR  - T32 ES007059/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20181115
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (Thyroid Hormones)
SB  - IM
MH  - Animals
MH  - Gene Expression Regulation, Developmental/*genetics
MH  - Thyroid Gland/*metabolism
MH  - Thyroid Hormones/*metabolism
MH  - Transcriptome/*genetics
MH  - Zebrafish/*genetics
PMC - PMC6331280
MID - NIHMS1515234
OTO - NOTNLM
OT  - Endocrine disruption
OT  - Thyroid hormone
OT  - Transcriptomics
OT  - Zebrafish
EDAT- 2018/11/19 06:00
MHDA- 2019/03/21 06:00
PMCR- 2020/02/01
CRDT- 2018/11/19 06:00
PHST- 2018/05/28 00:00 [received]
PHST- 2018/11/12 00:00 [revised]
PHST- 2018/11/14 00:00 [accepted]
PHST- 2018/11/19 06:00 [pubmed]
PHST- 2019/03/21 06:00 [medline]
PHST- 2018/11/19 06:00 [entrez]
PHST- 2020/02/01 00:00 [pmc-release]
AID - S0016-6480(18)30305-8 [pii]
AID - 10.1016/j.ygcen.2018.11.007 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2019 Feb 1;272:20-32. doi: 10.1016/j.ygcen.2018.11.007. Epub 
      2018 Nov 15.