PMID- 30451096
OWN - NLM
STAT- MEDLINE
DCOM- 20190411
LR  - 20200225
IS  - 1943-4936 (Electronic)
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 31
IP  - 1
DP  - 2019 Jan
TI  - Validation of a spectrophotometric method for GGT measurement in canine urine and 
      determination of the urine GGT-to-creatinine ratio reference interval and 
      biological variation in 41 healthy dogs.
PG  - 33-39
LID - 10.1177/1040638718812927 [doi]
AB  - The urine gamma-glutamyl transferase (GGT)-to-creatinine ratio has been used to 
      monitor patients at risk of acute renal injury. We validated the 
      spectrophotometric quantification of GGT in urine in a commercial biochemistry 
      analyzer. The assay was precise, accurate, and linear. Intra-assay precision was 
      3.59% in 4 samples, with GGT concentrations of 47-195 U/L. Inter-assay precision 
      in 3 samples with activities of 11-51 U/L was 7.74%. Accuracy was 97.3%, with an 
      absolute bias of 2.7 U/L. Urine GGT was unaffected by hematuria, hemoglobinuria, 
      or bacteriuria. Urine GGT was stable at 20 degrees C and 4 degrees C for up to 3 d. Storage by 
      freezing at -20 degrees C resulted in a significant reduction in enzyme activity. A pH 
      outside the range of 6.5-8 resulted in reduced GGT activity. The biological 
      variation of urine GGT-to-creatinine ratio provided an index of individuality of 
      1.6, indicating that a population-based reference interval (RI) can be used. The 
      reference change value was calculated, and an increase in consecutive 
      measurements >43% is required to be regarded as significant. The urine 
      GGT-to-creatinine ratio RI obtained in a population of 41 healthy dogs was 
      8.5-28.5 U/g.
FAU - Ilchyshyn, Nicholas P
AU  - Ilchyshyn NP
AD  - Dick White Diagnostics, Dick White Referrals, Six Mile Bottom, Cambridgeshire, 
      UK.
FAU - Villiers, Elizabeth
AU  - Villiers E
AD  - Dick White Diagnostics, Dick White Referrals, Six Mile Bottom, Cambridgeshire, 
      UK.
FAU - Monti, Paola
AU  - Monti P
AD  - Dick White Diagnostics, Dick White Referrals, Six Mile Bottom, Cambridgeshire, 
      UK.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20181118
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - AYI8EX34EU (Creatinine)
RN  - EC 2.3.2.2 (gamma-Glutamyltransferase)
SB  - IM
MH  - Animals
MH  - Creatinine/*urine
MH  - Dogs/*metabolism
MH  - England
MH  - Female
MH  - Male
MH  - Reference Values
MH  - Spectrophotometry/methods/*veterinary
MH  - Urinalysis/methods/*veterinary
MH  - Urine/chemistry
MH  - gamma-Glutamyltransferase/*urine
PMC - PMC6505752
OTO - NOTNLM
OT  - Acute kidney injury
OT  - GGT:creatinine ratio
OT  - creatinine
OT  - dogs
OT  - gamma-glutamyl transferase
OT  - urine
COIS- Declaration of conflicting interests: The authors declared no potential conflicts 
      of interest with respect to the research, authorship, and/or publication of this 
      article.
EDAT- 2018/11/20 06:00
MHDA- 2019/04/12 06:00
PMCR- 2019/11/18
CRDT- 2018/11/20 06:00
PHST- 2018/11/20 06:00 [pubmed]
PHST- 2019/04/12 06:00 [medline]
PHST- 2018/11/20 06:00 [entrez]
PHST- 2019/11/18 00:00 [pmc-release]
AID - 10.1177_1040638718812927 [pii]
AID - 10.1177/1040638718812927 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2019 Jan;31(1):33-39. doi: 10.1177/1040638718812927. Epub 
      2018 Nov 18.