PMID- 30453289
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20220430
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 51
IP  - 1
DP  - 2018
TI  - MicroRNA-197 Promotes Metastasis of Hepatocellular Carcinoma by Activating 
      Wnt/beta-Catenin Signaling.
PG  - 470-486
LID - 10.1159/000495242 [doi]
AB  - BACKGROUND/AIMS: MicroRNA-197 (miR-197) has been shown to play roles in 
      epithelialmesenchymal transition (EMT) and metastasis. The Wnt/beta-catenin pathway 
      is associated with EMT, but whether miR-197 regulatesWnt/beta-catenin remains 
      unclear. This study was to demonstrate the role of miR-197 on the Wnt/beta-catenin 
      pathway in hepatocellular carcinoma (HCC). METHODS: Quantitative reverse 
      transcription polymerase chain reaction (qRT-PCR) was used to detect the 
      expression of miR-197 in 105 HCC specimens and 15 HCC cell lines. We tested the 
      predicted target gene of miR-197 using a genetic report system. The role of 
      miR-197 in HCC cell invasion and migration (wound healingand cell invasion and 
      migrationby Transwell assays) and in an HCC xenograft modelwas analyzed. RESULTS: 
      Using a miRNA microarray analysis of HCC specimens and compared with 
      non-metastatic HCC, miR-197 was identified as one of the most upregulated miRNAs 
      in metastatic HCC. miR-197 expression was positively associated with the 
      invasiveness of HCC cell lines. Metastatic HCC cells with high miR-197 expression 
      had Wnt/beta-catenin signaling activation. High levels of miR-197 expression also 
      promoted EMT and invasionHCC cells in vitro and in vivo. miR-197 directly 
      targeted Axin-2, Naked cuticle 1 (NKD1), and Dickkopf-related protein 2 (DKK2), 
      leading to inhibition of Wnt/beta-catenin signaling. High miR-197 expression was 
      found in HCC specimens from patients with portal vein metastasis;high miR-197 
      expression correlated to the expression of Axin2, NKD1, and DKK2. CONCLUSION: 
      miR-197 promotes HCC invasion and metastasis by activating Wnt/beta-catenin 
      signaling. miR-197 could possibly be used as a prognostic marker and therapeutic 
      target for HCC.
CI  - (c) 2018 The Author(s). Published by S. Karger AG, Basel.
FAU - Hu, Zhaoxia
AU  - Hu Z
AD  - Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, China.
AD  - Key Laboratory of Tropical Disease Control, Ministry of Education, Sun Yat-sen 
      University, Guangzhou, China.
AD  - Guangdong Provincial Key Laboratory of Liver Disease, Guangzhou, China.
FAU - Wang, Peipei
AU  - Wang P
AD  - Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Lin, Jiaxin
AU  - Lin J
AD  - Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Zheng, Xingrong
AU  - Zheng X
AD  - Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Yang, Fangji
AU  - Yang F
AD  - Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Zhang, Genglin
AU  - Zhang G
AD  - Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Chen, Dabiao
AU  - Chen D
AD  - Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Xie, Junqiang
AU  - Xie J
AD  - Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Gao, Zhiliang
AU  - Gao Z
AD  - Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, China.
AD  - Key Laboratory of Tropical Disease Control, Ministry of Education, Sun Yat-sen 
      University, Guangzhou, China.
AD  - Guangdong Provincial Key Laboratory of Liver Disease, Guangzhou, China.
FAU - Peng, Liang
AU  - Peng L
AD  - Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Xie, Chan
AU  - Xie C
AD  - Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-Sen 
      University, Guangzhou, Chinaxchan@mail.sysu.edu.cn.
AD  - Key Laboratory of Tropical Disease Control, Ministry of Education, Sun Yat-sen 
      University, Guangzhou, Chinaxchan@mail.sysu.edu.cn.
AD  - Guangdong Provincial Key Laboratory of Liver Disease, Guangzhou, 
      Chinaxchan@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20181119
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (3' Untranslated Regions)
RN  - 0 (AXIN2 protein, human)
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Antagomirs)
RN  - 0 (Axin Protein)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (DKK2 protein, human)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (MIRN197 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NKD1 protein, human)
RN  - 0 (RNA, Small Interfering)
SB  - IM
EIN - Cell Physiol Biochem. 2022 Apr 30;56(2):229. PMID: 35488525
MH  - 3' Untranslated Regions
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Antagomirs/metabolism/therapeutic use
MH  - Axin Protein/antagonists & inhibitors/genetics/metabolism
MH  - Calcium-Binding Proteins
MH  - Carcinoma, Hepatocellular/drug therapy/genetics/*pathology
MH  - Carrier Proteins/antagonists & inhibitors/genetics/metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Epithelial-Mesenchymal Transition
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/chemistry/genetics/metabolism
MH  - Liver Neoplasms/drug therapy/genetics/*pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/antagonists & inhibitors/genetics/*metabolism
MH  - RNA Interference
MH  - RNA, Small Interfering/metabolism
MH  - *Wnt Signaling Pathway
OTO - NOTNLM
OT  - EMT
OT  - Hepatocellular carcinoma
OT  - Metastasis
OT  - MicroRNA
OT  - Wnt/b-catenin
EDAT- 2018/11/20 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/11/20 06:00
PHST- 2018/04/28 00:00 [received]
PHST- 2018/11/09 00:00 [accepted]
PHST- 2018/11/20 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/11/20 06:00 [entrez]
AID - 000495242 [pii]
AID - 10.1159/000495242 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2018;51(1):470-486. doi: 10.1159/000495242. Epub 2018 Nov 
      19.