PMID- 30454973 OWN - NLM STAT- MEDLINE DCOM- 20200102 LR - 20211204 IS - 1879-1476 (Electronic) IS - 0385-8146 (Linking) VI - 46 IP - 4 DP - 2019 Aug TI - MiR-196b affects the progression and prognosis of human LSCC through targeting PCDH-17. PG - 583-592 LID - S0385-8146(18)30764-8 [pii] LID - 10.1016/j.anl.2018.10.020 [doi] AB - OBJECTIVE: To explore the effect of miR-196bon the biological features of human laryngeal squamous cell carcinoma (LSCC) through targeting PCDH-17. METHODS: miR-196b and PCDH-17 expressions were determined in tissues, and the targeting relation of miR-196b and PCDH-17 was verified through dual-luciferase reporter system. In vitro, Hep-2 cells were divided into the Control, miR-196b inhibitors, miR-NC, PCDH-17, and miR-196b mimics+PCDH-17 groups. The miR-196b and PCDH-17 expressions were determined by qRT-PCR or/and Western blot, and the biological features by MTT, Annexin V-FITC/PI, wound-healing and Transwell assays. RESULTS: MiR-196b was found to be up-regulated, while PCDH-17 was down-regulated in a negative correlation in LSCC patients, which was related to histological grade and TNM stage. And low expression of miR-196b and high expression of PCDH-17 contributed to an increase in the 5-year-survival rate of LSCC patients. Besides, miR-196b directly targeted PCDH-17, while miR-196b inhibitors could up-regulate the PCDH-17 in Hep-2 cells. Moreover, miR-196b inhibitors and PCDH-17 curbed Hep-2 cell proliferation but facilitated the apoptosis, with decreases in cell invasion and migration. In addition, no statistical significance was found in cell proliferation, apoptosis, invasion and migration between Control group and miR-196b mimics+PCDH-17 group. CONCLUSION: LSCC patients exhibited the up-regulated miR-196b and down-regulated PCDH-17, which are correlated with the major clinical features and prognosis. Inhibiting miR-196b may suppress proliferation, migration and invasion abilities, and promote apoptosis of Hep-2 cells via targeting PCDH-17. CI - Copyright (c) 2018 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Luo, Min AU - Luo M AD - Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Provincial Hospital of Medical University of Anhui, Hefei 230001, Anhui, China. FAU - Sun, Gang AU - Sun G AD - Department of Otorhinolaryngology Head and Neck Surgery, Chaohu Hospital Affiliated to Medical University of Anhui, Chaohu 238000, Anhui, China. FAU - Sun, Jing-Wu AU - Sun JW AD - Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Provincial Hospital of Medical University of Anhui, Hefei 230001, Anhui, China. Electronic address: sunsunsjw@163.com. LA - eng PT - Journal Article DEP - 20181116 PL - Netherlands TA - Auris Nasus Larynx JT - Auris, nasus, larynx JID - 7708170 RN - 0 (Cadherins) RN - 0 (MIRN196 microRNA, human) RN - 0 (MicroRNAs) RN - 0 (PCDH17 protein, human) SB - IM MH - Adult MH - Aged MH - Aged, 80 and over MH - Apoptosis/genetics MH - Cadherins/*metabolism MH - Cell Line, Tumor MH - Cell Movement/genetics MH - Cell Proliferation/genetics MH - Disease Progression MH - Female MH - Humans MH - Laryngeal Neoplasms/*metabolism/pathology MH - Male MH - MicroRNAs/antagonists & inhibitors/*metabolism MH - Middle Aged MH - Neoplasm Staging MH - Prognosis MH - Squamous Cell Carcinoma of Head and Neck/*metabolism/pathology MH - Up-Regulation OTO - NOTNLM OT - LSCC OT - PCDH-17 OT - Prognosis OT - miR-196b EDAT- 2018/11/21 06:00 MHDA- 2020/01/03 06:00 CRDT- 2018/11/21 06:00 PHST- 2018/08/24 00:00 [received] PHST- 2018/10/09 00:00 [revised] PHST- 2018/10/30 00:00 [accepted] PHST- 2018/11/21 06:00 [pubmed] PHST- 2020/01/03 06:00 [medline] PHST- 2018/11/21 06:00 [entrez] AID - S0385-8146(18)30764-8 [pii] AID - 10.1016/j.anl.2018.10.020 [doi] PST - ppublish SO - Auris Nasus Larynx. 2019 Aug;46(4):583-592. doi: 10.1016/j.anl.2018.10.020. Epub 2018 Nov 16.