PMID- 30456489
OWN - NLM
STAT- MEDLINE
DCOM- 20200116
LR  - 20200116
IS  - 1432-0711 (Electronic)
IS  - 0932-0067 (Linking)
VI  - 299
IP  - 1
DP  - 2019 Jan
TI  - Seminal plasma (SP) induces a rapid transforming growth factor beta 1 
      (TGFbeta1)-independent up-regulation of epithelial-mesenchymal transdifferentiation 
      (EMT) and myofibroblastic metaplasia-markers in endometriotic (EM) and 
      endometrial cells.
PG  - 173-183
LID - 10.1007/s00404-018-4965-4 [doi]
AB  - PURPOSE: To study if short-term exposure (2 h and 6 h) of 
      endometrial/endometriotic tissues and cells to 10% seminal plasma (SP) can induce 
      EMT/metaplasia. METHODS: Basic research experimental study was carried out in a 
      University hospital-based fertility center. Semen samples, peritoneal fluid (PF) 
      from endometriosis patients, endometrial biopsy from premenopausal women, 
      immortalized endometriotic epithelial cell line (12Z), and immortalized 
      endometrial stromal cell line (St-T1b) were studied. Rapid stain identification 
      test (RSID), TGFbeta1 immunofluorescence of washed sperms, TGFbeta1-ELISA of SP and PF, 
      in vitro study (2 h and 6 h incubation) and real-time PCR of endometrial tissue 
      and cell lines to analyze gene expression of EMT/metaplasia markers and mediators 
      were done. RESULTS: SP is still detectable in washed semen. TGFbeta1 was expressed 
      on the plasma membrane of the sperms and was significantly more concentrated in 
      SP (88.17 ng/ml) than PF. 10% SP induced an up-regulation of alpha smooth muscle 
      actin expression in endometrial tissue (p = 0.008) and in 12Z cells (p = 0.05), 
      mostly TGFbeta1-independent. TWIST expression was persistently significantly 
      down-regulated while Snail1 and 2 were up-regulated, though insignificant. 
      CONCLUSION: Our results provide novel evidence to support that even in semen 
      washed twice, SP is still detectable. The changes in EMT/metaplasia markers and 
      mediators give a new insight into a possible effect of SP on the pathogenesis of 
      endometriosis.
FAU - Ibrahim, M G
AU  - Ibrahim MG
AUID- ORCID: 0000-0001-6648-9281
AD  - UKM Fertility Center, Department of Gynecology and Obstetrics, University 
      Hospital of Muenster, Albert-Schweitzer-Campus 1, D-11, 48149, Muenster, Germany. 
      mohamed.Ibrahim@ukmuenster.de.
FAU - Elghonaimy, E A
AU  - Elghonaimy EA
AD  - Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.
AD  - Department of Gynecology and Obstetrics, University Hospital of Muenster, 
      Albert-Schweitzer-Campus 1, A-1, Muenster, Germany.
FAU - Schafer, S
AU  - Schafer S
AD  - Department of Gynecology and Obstetrics, University Hospital of Muenster, 
      Albert-Schweitzer-Campus 1, A-1, Muenster, Germany.
FAU - Vennemann, M
AU  - Vennemann M
AD  - Department of Legal Medicine, University Hospital of Muenster, Muenster, Germany.
FAU - Kliesch, S
AU  - Kliesch S
AD  - Center of Reproductive Medicine and Andrology (CeRA), Department of Clinical and 
      Surgical Andrology, University Hospital of Muenster, Muenster, Germany.
FAU - Kiesel, L
AU  - Kiesel L
AD  - Department of Gynecology and Obstetrics, University Hospital of Muenster, 
      Albert-Schweitzer-Campus 1, A-1, Muenster, Germany.
FAU - Gotte, M
AU  - Gotte M
AD  - Department of Gynecology and Obstetrics, University Hospital of Muenster, 
      Albert-Schweitzer-Campus 1, A-1, Muenster, Germany.
FAU - Schuring, A N
AU  - Schuring AN
AD  - UKM Fertility Center, Department of Gynecology and Obstetrics, University 
      Hospital of Muenster, Albert-Schweitzer-Campus 1, D-11, 48149, Muenster, Germany.
LA  - eng
PT  - Journal Article
DEP - 20181119
PL  - Germany
TA  - Arch Gynecol Obstet
JT  - Archives of gynecology and obstetrics
JID - 8710213
RN  - 0 (Biomarkers)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Ascitic Fluid/metabolism
MH  - Biomarkers/metabolism
MH  - Cell Proliferation
MH  - *Cell Transdifferentiation
MH  - Endometriosis/metabolism/*pathology
MH  - Endometrium/pathology
MH  - Epithelial Cells/metabolism
MH  - Epithelial-Mesenchymal Transition
MH  - Female
MH  - Humans
MH  - Metaplasia
MH  - Semen/*physiology
MH  - Stromal Cells/metabolism
MH  - Transforming Growth Factor beta1/*metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - EMT
OT  - Endometriosis
OT  - Metaplasia
OT  - Seminal plasma
OT  - TGFbeta1
EDAT- 2018/11/21 06:00
MHDA- 2020/01/17 06:00
CRDT- 2018/11/21 06:00
PHST- 2018/06/16 00:00 [received]
PHST- 2018/11/08 00:00 [accepted]
PHST- 2018/11/21 06:00 [pubmed]
PHST- 2020/01/17 06:00 [medline]
PHST- 2018/11/21 06:00 [entrez]
AID - 10.1007/s00404-018-4965-4 [pii]
AID - 10.1007/s00404-018-4965-4 [doi]
PST - ppublish
SO  - Arch Gynecol Obstet. 2019 Jan;299(1):173-183. doi: 10.1007/s00404-018-4965-4. 
      Epub 2018 Nov 19.