PMID- 30458119
OWN - NLM
STAT- MEDLINE
DCOM- 20190401
LR  - 20190401
IS  - 1946-6544 (Electronic)
IS  - 1946-6536 (Linking)
VI  - 29
IP  - 6
DP  - 2018 Dec
TI  - Transparenchymal Renal Pelvis Injection of Recombinant Adeno-Associated Virus 
      Serotype 9 Vectors Is a Practical Approach for Gene Delivery in the Kidney.
PG  - 251-258
LID - 10.1089/hgtb.2018.148 [doi]
AB  - Gene therapy has great potential in treating human diseases, but little progress 
      has been made in preclinical and clinical studies of renal diseases. To find an 
      effective gene delivery approach in the kidney, transparenchymal renal pelvis 
      injection was developed. Using adeno-associated virus serotype 9 (AAV9) vectors, 
      the gene delivery efficiency and safety of this administration method were 
      evaluated. The results showed that the exogenous gene was expressed in the 
      tubular epithelial cells of the injected kidney, with a much lower expression 
      level in the contralateral kidney. Extra-renal transduction in the liver was also 
      observed in this study, with the liver function of AAV9-injected mice comparable 
      to that of control mice. Altogether, the administration of AAV9 vectors by newly 
      established transparenchymal renal pelvis injection achieved the desired 
      exogenous gene expression in renal tubular cells, and hence might be one possible 
      way for gene therapy in renal diseases.
FAU - Shen, Xufeng
AU  - Shen X
AD  - 1 Department of Urology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei, P.R. China; Hefei, P.R. China.
AD  - 2 Institute of Urology and Hefei, P.R. China.
AD  - 3 Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical 
      University, Hefei, P.R. China; and Hefei, P.R. China.
AD  - 4 Anhui Province PKD Center, Hefei, P.R. China.
FAU - Xu, Yuchen
AU  - Xu Y
AD  - 1 Department of Urology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei, P.R. China; Hefei, P.R. China.
AD  - 2 Institute of Urology and Hefei, P.R. China.
AD  - 3 Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical 
      University, Hefei, P.R. China; and Hefei, P.R. China.
AD  - 4 Anhui Province PKD Center, Hefei, P.R. China.
FAU - Bai, Zhengming
AU  - Bai Z
AD  - 1 Department of Urology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei, P.R. China; Hefei, P.R. China.
AD  - 2 Institute of Urology and Hefei, P.R. China.
AD  - 3 Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical 
      University, Hefei, P.R. China; and Hefei, P.R. China.
AD  - 4 Anhui Province PKD Center, Hefei, P.R. China.
FAU - Ma, Dongyue
AU  - Ma D
AD  - 1 Department of Urology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei, P.R. China; Hefei, P.R. China.
AD  - 2 Institute of Urology and Hefei, P.R. China.
AD  - 3 Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical 
      University, Hefei, P.R. China; and Hefei, P.R. China.
AD  - 4 Anhui Province PKD Center, Hefei, P.R. China.
FAU - Niu, Qingsong
AU  - Niu Q
AD  - 1 Department of Urology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei, P.R. China; Hefei, P.R. China.
AD  - 2 Institute of Urology and Hefei, P.R. China.
AD  - 3 Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical 
      University, Hefei, P.R. China; and Hefei, P.R. China.
AD  - 4 Anhui Province PKD Center, Hefei, P.R. China.
FAU - Meng, Jialin
AU  - Meng J
AD  - 1 Department of Urology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei, P.R. China; Hefei, P.R. China.
AD  - 2 Institute of Urology and Hefei, P.R. China.
AD  - 3 Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical 
      University, Hefei, P.R. China; and Hefei, P.R. China.
FAU - Fan, Song
AU  - Fan S
AD  - 1 Department of Urology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei, P.R. China; Hefei, P.R. China.
AD  - 2 Institute of Urology and Hefei, P.R. China.
AD  - 3 Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical 
      University, Hefei, P.R. China; and Hefei, P.R. China.
FAU - Zhang, Li
AU  - Zhang L
AD  - 1 Department of Urology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei, P.R. China; Hefei, P.R. China.
AD  - 2 Institute of Urology and Hefei, P.R. China.
AD  - 3 Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical 
      University, Hefei, P.R. China; and Hefei, P.R. China.
FAU - Hao, Zongyao
AU  - Hao Z
AD  - 1 Department of Urology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei, P.R. China; Hefei, P.R. China.
AD  - 2 Institute of Urology and Hefei, P.R. China.
AD  - 3 Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical 
      University, Hefei, P.R. China; and Hefei, P.R. China.
FAU - Zhang, Xiansheng
AU  - Zhang X
AD  - 1 Department of Urology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei, P.R. China; Hefei, P.R. China.
AD  - 2 Institute of Urology and Hefei, P.R. China.
AD  - 3 Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical 
      University, Hefei, P.R. China; and Hefei, P.R. China.
FAU - Liang, Chaozhao
AU  - Liang C
AD  - 1 Department of Urology, the First Affiliated Hospital of Anhui Medical 
      University, Hefei, P.R. China; Hefei, P.R. China.
AD  - 2 Institute of Urology and Hefei, P.R. China.
AD  - 3 Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical 
      University, Hefei, P.R. China; and Hefei, P.R. China.
AD  - 4 Anhui Province PKD Center, Hefei, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Gene Ther Methods
JT  - Human gene therapy methods
JID - 101573202
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Dependovirus/*genetics/metabolism
MH  - Epithelial Cells/metabolism
MH  - *Gene Transfer Techniques
MH  - Genetic Therapy/*methods
MH  - Genetic Vectors/genetics
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Injections/methods
MH  - Kidney/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
OTO - NOTNLM
OT  - AAV
OT  - gene delivery
OT  - mouse model
OT  - renal disease
EDAT- 2018/11/21 06:00
MHDA- 2019/04/02 06:00
CRDT- 2018/11/21 06:00
PHST- 2018/11/21 06:00 [pubmed]
PHST- 2019/04/02 06:00 [medline]
PHST- 2018/11/21 06:00 [entrez]
AID - 10.1089/hgtb.2018.148 [doi]
PST - ppublish
SO  - Hum Gene Ther Methods. 2018 Dec;29(6):251-258. doi: 10.1089/hgtb.2018.148.