PMID- 30459422
OWN - NLM
STAT- MEDLINE
DCOM- 20191217
LR  - 20240403
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Nov 20
TI  - Cell wall mannan of Candida krusei mediates dendritic cell apoptosis and 
      orchestrates Th17 polarization via TLR-2/MyD88-dependent pathway.
PG  - 17123
LID - 10.1038/s41598-018-35101-3 [doi]
LID - 17123
AB  - Dendritic cells (DCs) abundantly express diverse receptors to recognize mannans 
      in the outer surface of Candida cell wall, and these interactions dictate the 
      host immune responses that determine disease outcomes. C. krusei prevalence in 
      candidiasis worldwide has increased since this pathogen has developed multidrug 
      resistance. However, little is known how the immune system responds to C. krusei. 
      Particularly, the molecular mechanisms of the interplay between C. krusei mannan 
      and DCs remain to be elucidated. We investigated how C. krusei mannan affected DC 
      responses in comparison to C. albicans, C. tropicalis and C. glabrata mannan. Our 
      results showed that only C. krusei mannan induced massive cytokine responses in 
      DCs, and led to apoptosis. Although C. krusei mannan-activated DCs underwent 
      apoptosis, they were still capable of initiating Th17 response. C. krusei 
      mannan-mediated DC apoptosis was obligated to the TLR2 and MyD88 pathway. These 
      pathways also controlled Th1/Th17 switching possibly by virtue of the production 
      of the polarizing cytokines IL-12 and IL-6 by the C. krusei mannan activated-DCs. 
      Our study suggests that TLR2 and MyD88 pathway in DCs are dominant for C. krusei 
      mannan recognition, which differs from the previous reports showing a crucial 
      role of C-type lectin receptors in Candida mannan sensing.
FAU - Nguyen, Thu Ngoc Yen
AU  - Nguyen TNY
AUID- ORCID: 0000-0003-1329-7299
AD  - Graduate program in Oral Biology, Faculty of Dentistry, Chulalongkorn University, 
      Bangkok, 10330, Thailand.
FAU - Padungros, Panuwat
AU  - Padungros P
AD  - Organic Synthesis Research Unit, Department of Chemistry, Faculty of Science, 
      Chulalongkorn University, Phayathai Road, Patumwan, Bangkok, 10330, Thailand.
FAU - Wongsrisupphakul, Panachai
AU  - Wongsrisupphakul P
AD  - Organic Synthesis Research Unit, Department of Chemistry, Faculty of Science, 
      Chulalongkorn University, Phayathai Road, Patumwan, Bangkok, 10330, Thailand.
FAU - Sa-Ard-Iam, Noppadol
AU  - Sa-Ard-Iam N
AD  - Immunology Laboratory, Faculty of Dentistry, Chulalongkorn University, Bangkok, 
      10330, Thailand.
FAU - Mahanonda, Rangsini
AU  - Mahanonda R
AD  - Immunology Laboratory, Faculty of Dentistry, Chulalongkorn University, Bangkok, 
      10330, Thailand.
AD  - Department of Periodontology, Faculty of Dentistry, Chulalongkorn University, 
      Bangkok, 10330, Thailand.
FAU - Matangkasombut, Oranart
AU  - Matangkasombut O
AD  - Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok, 10210, 
      Thailand.
AD  - Research Unit on Oral Microbiology and Immunology and Department of Microbiology, 
      Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand.
FAU - Choo, Min-Kyung
AU  - Choo MK
AUID- ORCID: 0000-0003-1492-7539
AD  - Cutaneous Biology Research Center, Massachusetts General Hospital and Harvard 
      Medical School, Charlestown, MA, 02129, USA.
FAU - Ritprajak, Patcharee
AU  - Ritprajak P
AD  - Research Unit on Oral Microbiology and Immunology and Department of Microbiology, 
      Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand. 
      Patcharee.R@chula.ac.th.
AD  - Oral Biology Research Center, Faculty of Dentistry, Chulalongkorn University, 
      Bangkok, Thailand. Patcharee.R@chula.ac.th.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181120
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Cytokines)
RN  - 0 (Mannans)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Tlr2 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects/immunology
MH  - Candida/chemistry/cytology/*pathogenicity
MH  - Candidiasis/metabolism/microbiology/pathology
MH  - Cell Wall/chemistry
MH  - Cytokines/metabolism
MH  - Dendritic Cells/drug effects/*immunology/metabolism
MH  - Host-Pathogen Interactions
MH  - Mannans/*toxicity
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Myeloid Differentiation Factor 88/metabolism
MH  - Th17 Cells/drug effects/*immunology/metabolism
MH  - Toll-Like Receptor 2/metabolism
PMC - PMC6244250
COIS- The authors declare no competing interests.
EDAT- 2018/11/22 06:00
MHDA- 2019/12/18 06:00
PMCR- 2018/11/20
CRDT- 2018/11/22 06:00
PHST- 2018/01/17 00:00 [received]
PHST- 2018/10/23 00:00 [accepted]
PHST- 2018/11/22 06:00 [entrez]
PHST- 2018/11/22 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2018/11/20 00:00 [pmc-release]
AID - 10.1038/s41598-018-35101-3 [pii]
AID - 35101 [pii]
AID - 10.1038/s41598-018-35101-3 [doi]
PST - epublish
SO  - Sci Rep. 2018 Nov 20;8(1):17123. doi: 10.1038/s41598-018-35101-3.