PMID- 30459721
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 9
DP  - 2018
TI  - Association of the Human Bocavirus With Tonsil Squamous Cell Carcinomas.
PG  - 2450
LID - 10.3389/fmicb.2018.02450 [doi]
LID - 2450
AB  - Background: The human bocavirus (HBoV) is known to persist latently in the 
      infected host cells and seems to replicate its DNA via the DNA damage response 
      system, which is frequently defect in tumors and correlates with microsatellite 
      instability (MSI). Because HBoV is able to persist in the infected tissues, 
      induces pro-fibrotic and pro- cancerogenic cytokines in vivo and in vitro, and is 
      detected in colorectal and lung tumors, the virus may be involved in 
      cancerogenesis at least as a cofactor. Recently it was shown that the 
      adenotonsillar tissue is an important site of HBoV1 persistence and replication. 
      Considering the background that approximately 60% of oropharyngeal cancers were 
      thought to be attributable to a HPV infection, a co-participation of HBoV in 
      terms of a chronic virus infection might play a role in the cancerogenesis of 
      tonsil tumors. Methods: Formalin-fixed, paraffin-embedded tonsil tumor samples 
      were screened for HBoV and HPV DNA. Positive tissue sections were afterward 
      subjected to fluorescence in situ hybridization (FISH) analysis to identify HBoV 
      and HPV infected cells. By use of an in vitro cell culture model with primary 
      tonsil fibroblasts, keratinocytes, and lymphocytes infected by HBoV we tried to 
      find the target cells of virus replication. MSI testing was based on a previously 
      published protocol using a de-multiplexed PCR followed by fluorescent detection 
      of PCR products in a capillary sequencing device. Results: In total 62 of 103 
      (60, 19%) of the tonsil squamous cell carcinomas tested positive for HBoV DNA and 
      66 of 103 (66%) samples were identified as HPV positive. The FISH analysis 
      revealed both double infection of HPV and HBoV in the same cells as well as 
      single infections of both viruses within the tumor tissue. Twenty-two of 62 HBoV 
      positive tumors tested HPV negative, 40 of 62 tissue sections were HBoV and HPV 
      positive. We analyzed 21 out of the 62 HBoV positive tumors for MSI. Of those 
      four tonsils displayed MSI in at least 1 of 10 microsatellite markers. 
      Conclusion: Our findings support the hypothesis that human bocavirus infections 
      as a cofactor may have an impact on tumor development in tonsils, although it 
      still remains possible that HBoV solely displays a tumor tropism.
FAU - Hopken, Merle
AU  - Hopken M
AD  - Klinik fur Hals-, Nasen- und Ohrenheilkunde, Kliniken der Stadt Koln, Klinikum 
      der Privaten Universitat Witten/Herdecke mit Sitz in Koln, Cologne, Germany.
AD  - Institut fur Pathologie, Kliniken der Stadt Koln, Klinikum der Privaten 
      Universitat Witten/Herdecke mit Sitz in Koln, Cologne, Germany.
FAU - Forster, Isabel
AU  - Forster I
AD  - Institut fur Pathologie, Kliniken der Stadt Koln, Klinikum der Privaten 
      Universitat Witten/Herdecke mit Sitz in Koln, Cologne, Germany.
FAU - Maune, Steffen
AU  - Maune S
AD  - Klinik fur Hals-, Nasen- und Ohrenheilkunde, Kliniken der Stadt Koln, Klinikum 
      der Privaten Universitat Witten/Herdecke mit Sitz in Koln, Cologne, Germany.
FAU - Brockmann, Michael
AU  - Brockmann M
AD  - Institut fur Pathologie, Kliniken der Stadt Koln, Klinikum der Privaten 
      Universitat Witten/Herdecke mit Sitz in Koln, Cologne, Germany.
FAU - Schildgen, Oliver
AU  - Schildgen O
AD  - Institut fur Pathologie, Kliniken der Stadt Koln, Klinikum der Privaten 
      Universitat Witten/Herdecke mit Sitz in Koln, Cologne, Germany.
FAU - Schildgen, Verena
AU  - Schildgen V
AD  - Institut fur Pathologie, Kliniken der Stadt Koln, Klinikum der Privaten 
      Universitat Witten/Herdecke mit Sitz in Koln, Cologne, Germany.
LA  - eng
PT  - Journal Article
DEP - 20181016
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC6232770
OTO - NOTNLM
OT  - human bocavirus
OT  - oncogenesis
OT  - persistence
OT  - primary cell culture
OT  - tonsil squamous cell carcinoma
EDAT- 2018/11/22 06:00
MHDA- 2018/11/22 06:01
PMCR- 2018/10/16
CRDT- 2018/11/22 06:00
PHST- 2018/06/28 00:00 [received]
PHST- 2018/09/25 00:00 [accepted]
PHST- 2018/11/22 06:00 [entrez]
PHST- 2018/11/22 06:00 [pubmed]
PHST- 2018/11/22 06:01 [medline]
PHST- 2018/10/16 00:00 [pmc-release]
AID - 10.3389/fmicb.2018.02450 [doi]
PST - epublish
SO  - Front Microbiol. 2018 Oct 16;9:2450. doi: 10.3389/fmicb.2018.02450. eCollection 
      2018.