PMID- 30459801
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 9
DP  - 2018
TI  - Comprehensively Characterizing the Cytological Features of Saccharum spontaneum 
      by the Development of a Complete Set of Chromosome-Specific Oligo Probes.
PG  - 1624
LID - 10.3389/fpls.2018.01624 [doi]
LID - 1624
AB  - Chromosome-specific identification is a powerful technique in the study of genome 
      structure and evolution. However, there is no reliable cytogenetic marker to 
      unambiguously identify each of the chromosomes in sugarcane (Saccharum spp., 
      Poaceae), which has a complex genome with a high level of ploidy and 
      heterozygosity. In this study, we developed a set of oligonucleotide 
      (oligo)-based probes through bioinformatic design and massive synthetization. 
      These probes produced a clear and bright single signal in each of the chromosomes 
      and their eight homologous chromosomes in the ancient species Saccharum 
      spontaneum (2n = 8x = 64). Thus, they can be used as reliable markers to robustly 
      label each of the chromosomes in S. spontaneum. We then obtained the karyotype 
      data and established a nomenclature based on chromosomal sizes for the eight 
      chromosomes of the octoploid S. spontaneum. In addition, we also found that the 
      45S and 5S rDNAs demonstrated high copy number variations among different 
      homologous chromosomes, indicating a rapid evolution of the highly repeated 
      sequence after polyploidization. Our fluorescence in situ hybridization (FISH) 
      assay also demonstrated that these probes could be used as cross-species markers 
      between or within the genera of Sorghum and Saccharum. By comparing FISH 
      analyses, we discovered that several chromosome rearrangement events occurred in 
      S. spontaneum, which might have contributed to the basic chromosome number 
      reduction from 10 in sorghum to 8 in sugarcane. Consistent identification of 
      individual chromosomes makes molecular cytogenetic study possible in sugarcane 
      and will facilitate fine chromosomal structure and karyotype evolution of the 
      genus Saccharum.
FAU - Meng, Zhuang
AU  - Meng Z
AD  - Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry 
      of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems 
      Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry 
      University, Fuzhou, China.
FAU - Zhang, Zhiliang
AU  - Zhang Z
AD  - Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry 
      of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems 
      Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry 
      University, Fuzhou, China.
FAU - Yan, Tianying
AU  - Yan T
AD  - Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry 
      of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems 
      Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry 
      University, Fuzhou, China.
FAU - Lin, Qingfang
AU  - Lin Q
AD  - Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry 
      of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems 
      Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry 
      University, Fuzhou, China.
FAU - Wang, Yu
AU  - Wang Y
AD  - Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry 
      of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems 
      Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry 
      University, Fuzhou, China.
FAU - Huang, Weiyuan
AU  - Huang W
AD  - Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry 
      of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems 
      Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry 
      University, Fuzhou, China.
FAU - Huang, Yongji
AU  - Huang Y
AD  - Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry 
      of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems 
      Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry 
      University, Fuzhou, China.
FAU - Li, Zhanjie
AU  - Li Z
AD  - Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry 
      of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems 
      Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry 
      University, Fuzhou, China.
FAU - Yu, Qingyi
AU  - Yu Q
AD  - Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry 
      of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems 
      Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry 
      University, Fuzhou, China.
AD  - Texas A&M AgriLife Research, The Texas A&M University System, Dallas, TX, United 
      States.
FAU - Wang, Jianping
AU  - Wang J
AD  - Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry 
      of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems 
      Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry 
      University, Fuzhou, China.
AD  - Department of Agronomy, University of Florida, Gainesville, FL, United States.
FAU - Wang, Kai
AU  - Wang K
AD  - Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry 
      of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems 
      Biology, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry 
      University, Fuzhou, China.
AD  - National Engineering Research Center of Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20181106
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC6232525
OTO - NOTNLM
OT  - Saccharum spontaneum
OT  - chromosomal rearrangement
OT  - chromosome identification
OT  - fluorescence in situ hybridization
OT  - karyotype
OT  - oligo probe
EDAT- 2018/11/22 06:00
MHDA- 2018/11/22 06:01
PMCR- 2018/01/01
CRDT- 2018/11/22 06:00
PHST- 2018/08/25 00:00 [received]
PHST- 2018/10/18 00:00 [accepted]
PHST- 2018/11/22 06:00 [entrez]
PHST- 2018/11/22 06:00 [pubmed]
PHST- 2018/11/22 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2018.01624 [doi]
PST - epublish
SO  - Front Plant Sci. 2018 Nov 6;9:1624. doi: 10.3389/fpls.2018.01624. eCollection 
      2018.