PMID- 30460727
OWN - NLM
STAT- MEDLINE
DCOM- 20190416
LR  - 20190416
IS  - 1439-0396 (Electronic)
IS  - 0931-2439 (Linking)
VI  - 103
IP  - 1
DP  - 2019 Jan
TI  - Effects of methionine on muscle protein synthesis and degradation pathways in 
      broilers.
PG  - 191-203
LID - 10.1111/jpn.13026 [doi]
AB  - This study investigated the hypothesis that supplementation of methionine (Met) 
      to broiler diets increases muscle growth due to regulation of molecular pathways 
      related to protein synthesis and degradation depending on the Met source. Day-old 
      male Cobb-500 broilers (n = 240) were phase-fed three different wheat-soya bean 
      meal-based basal diets during days 1-10, 11-21 and 22-35. Basal diets (Met- 
      group, Met + Cys concentration 15% below NRC recommendations) were supplemented 
      with 0.10% or 0.40% Met either as DL-Met (DLM) or DL-2-hydroxy-4-(methylthio) 
      butanoic acid (DL-HMTBA) (equimolar comparison). Breast muscle weights were lower 
      in the Met- group compared to all Met-supplemented groups and were lower in 
      broilers supplemented with 0.10% of DL-HMTBA compared to the other groups fed 
      Met-supplemented diets. However, the expression of genes or relative 
      phosphorylation and thus activation state of proteins involved in the 
      somatotropic axis, the mammalian target of rapamycin (mTOR) pathway of protein 
      synthesis, the ubiquitin-proteasome pathway (UPP) and autophagy-lysosomal pathway 
      of protein degradation, the GCN2/eIF2a pathway involved in the inhibition of 
      protein synthesis and in the myostatin-Smad2/3 pathway involved in myogenesis 
      were not affected by Met source. Feeding diets with suboptimum Met + Cys 
      concentrations, however, decreased expression of GHR and IGF1 in liver and muscle 
      and increased that of MURF1 involved in the UPP in the broiler's muscle at day 10 
      and 21, while that of FOXO and atrogin-1 and FOXO phosphorylation remained 
      unaffected. Additionally, suboptimum dietary Met concentrations increased 
      expression of the autophagy-related genes ATG5 and BECN1 at day 35. Met 
      supplementation neither affected gene expression nor phosphorylation of proteins 
      involved in the GNC2/eIF2a and mTOR pathways. These data indicate that protein 
      synthesis was not affected on the molecular level, while protein degradation was 
      marginally affected by dietary Met dosage.
CI  - (c) 2018 Blackwell Verlag GmbH.
FAU - Zeitz, Johanna O
AU  - Zeitz JO
AUID- ORCID: 0000-0003-4559-4036
AD  - Institute of Animal Nutrition and Nutrition Physiology, University of Giessen, 
      Giessen, Germany.
FAU - Mohrmann, Sarah
AU  - Mohrmann S
AD  - Institute of Animal Nutrition and Nutrition Physiology, University of Giessen, 
      Giessen, Germany.
FAU - Kading, Stella C
AU  - Kading SC
AD  - Institute of Animal Nutrition and Nutrition Physiology, University of Giessen, 
      Giessen, Germany.
FAU - Devlikamov, Murat
AU  - Devlikamov M
AD  - Institute of Animal Nutrition and Nutrition Physiology, University of Giessen, 
      Giessen, Germany.
FAU - Niewalda, Ines
AU  - Niewalda I
AD  - Institute of Animal Nutrition and Nutrition Physiology, University of Giessen, 
      Giessen, Germany.
FAU - Whelan, Rose
AU  - Whelan R
AD  - Evonik Nutrition & Care GmbH, Hanau-Wolfgang, Germany.
FAU - Helmbrecht, Ariane
AU  - Helmbrecht A
AD  - Evonik Nutrition & Care GmbH, Hanau-Wolfgang, Germany.
FAU - Eder, Klaus
AU  - Eder K
AUID- ORCID: 0000-0003-3246-4136
AD  - Institute of Animal Nutrition and Nutrition Physiology, University of Giessen, 
      Giessen, Germany.
LA  - eng
GR  - Evonik Nutrition & Care GmbH, Hanau-Wolfgang, Germany/
PT  - Clinical Trial, Veterinary
PT  - Journal Article
DEP - 20181120
PL  - Germany
TA  - J Anim Physiol Anim Nutr (Berl)
JT  - Journal of animal physiology and animal nutrition
JID - 101126979
RN  - 0 (Muscle Proteins)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animal Feed/analysis
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Chickens/*metabolism
MH  - Diet/veterinary
MH  - Male
MH  - Methionine/*metabolism
MH  - Muscle Proteins/genetics/*metabolism
MH  - Muscle, Skeletal/*drug effects/metabolism
OTO - NOTNLM
OT  - DL-methionine
OT  - broiler
OT  - mTOR
OT  - methionine hydroxyl analogue
OT  - protein degradation
EDAT- 2018/11/22 06:00
MHDA- 2019/04/17 06:00
CRDT- 2018/11/22 06:00
PHST- 2018/09/04 00:00 [received]
PHST- 2018/10/18 00:00 [revised]
PHST- 2018/10/21 00:00 [accepted]
PHST- 2018/11/22 06:00 [pubmed]
PHST- 2019/04/17 06:00 [medline]
PHST- 2018/11/22 06:00 [entrez]
AID - 10.1111/jpn.13026 [doi]
PST - ppublish
SO  - J Anim Physiol Anim Nutr (Berl). 2019 Jan;103(1):191-203. doi: 10.1111/jpn.13026. 
      Epub 2018 Nov 20.