PMID- 30460873
OWN - NLM
STAT- MEDLINE
DCOM- 20190624
LR  - 20190624
IS  - 1473-7760 (Electronic)
IS  - 1052-0295 (Linking)
VI  - 94
IP  - 3
DP  - 2019 Apr
TI  - Keratinocytes derived from embryonic stem cells induce wound healing in mice.
PG  - 189-198
LID - 10.1080/10520295.2018.1541479 [doi]
AB  - The skin plays an important role in defending the body against the environment. 
      Treatments for burns and skin injuries that use autologous or allogenic skin 
      grafts derived from adult or embryonic stem cells are promising. Embryonic stem 
      cells are candidates for regenerative and reparative medicine. We investigated 
      the utility of keratinocyte-like cells, which are differentiated from mouse 
      embryonic stem cells, for wound healing using a mouse surgical wound model. Mice 
      were allocated to the following groups: experimental, in which dressing and 
      differentiated cells were applied after the surgical wound was created; control, 
      in which only the surgical wound was created; sham, in which only the dressing 
      was applied after the surgical wound was created; and untreated animal controls 
      with healthy skin. Biopsies were taken from each group on days 3, 5 and 7 after 
      cell transfer. Samples were fixed in formalin, then stained with Masson's 
      trichrome and primary antibodies to interleukin-8 (IL-8), fibroblast growth 
      factor-2 (FGF-2), monocyte chemoattractant protein-1 (MCP-1), collagen-1 and 
      epidermal growth factor (EGF) using the indirect immunoperoxidase technique for 
      light microscopy. Wound healing was faster in the experimental group compared to 
      the sham and control groups. The experimental group exhibited increased 
      expression of IL-8, FGF-2 and MCP-1 during early stages of wound healing 
      (inflammation) and collagen-1 and EGF expression during late stages of wound 
      healing (proliferation and remodeling). Keratinocytes derived from embryonic stem 
      cells improved wound healing and influenced the wound healing stages.
FAU - Uluer, E T
AU  - Uluer ET
AD  - a Departments of Histology and Embryology, Faculty of Medicine , Manisa Celal 
      Bayar University , Manisa , Turkey.
FAU - Vatansever, H S
AU  - Vatansever HS
AD  - a Departments of Histology and Embryology, Faculty of Medicine , Manisa Celal 
      Bayar University , Manisa , Turkey.
FAU - Aydede, H
AU  - Aydede H
AD  - b Departments of General Surgery, Faculty of Medicine , Manisa Celal Bayar 
      University , Manisa , Turkey.
FAU - Ozbilgin, M K
AU  - Ozbilgin MK
AD  - a Departments of Histology and Embryology, Faculty of Medicine , Manisa Celal 
      Bayar University , Manisa , Turkey.
LA  - eng
PT  - Journal Article
DEP - 20181121
PL  - England
TA  - Biotech Histochem
JT  - Biotechnic & histochemistry : official publication of the Biological Stain 
      Commission
JID - 9107378
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Chemokine CCL2/genetics/metabolism
MH  - Collagen/metabolism
MH  - Embryonic Stem Cells/*physiology
MH  - Epidermal Growth Factor/genetics/metabolism
MH  - Fibroblast Growth Factor 2/genetics/metabolism
MH  - Interleukin-8/genetics/metabolism
MH  - Keratinocytes/*physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Wound Healing/*physiology
OTO - NOTNLM
OT  - Collagen-1
OT  - embryonic stem cell
OT  - epidermal growth factor
OT  - fibroblast growth factor-2
OT  - interleukin-8
OT  - keratinocytes
OT  - monocyte chemoattractant protein-1
OT  - mouse
OT  - regeneration
OT  - wound healing
EDAT- 2018/11/22 06:00
MHDA- 2019/06/25 06:00
CRDT- 2018/11/22 06:00
PHST- 2018/11/22 06:00 [pubmed]
PHST- 2019/06/25 06:00 [medline]
PHST- 2018/11/22 06:00 [entrez]
AID - 10.1080/10520295.2018.1541479 [doi]
PST - ppublish
SO  - Biotech Histochem. 2019 Apr;94(3):189-198. doi: 10.1080/10520295.2018.1541479. 
      Epub 2018 Nov 21.