PMID- 30463065
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20211102
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 51
IP  - 2
DP  - 2018
TI  - MicroRNA-485 Modulates the TGF-beta/ Smads Signaling Pathway in Chronic Asthmatic 
      Mice by Targeting Smurf2.
PG  - 692-710
LID - 10.1159/000495327 [doi]
AB  - BACKGROUND/AIMS: Chronic respiratory conditions continue to plague millions of 
      people worldwide. We aimed to elucidate the detailed mechanisms of microRNA-485 
      (miR-485) in airway smooth muscle cell (ASMC) proliferation and apoptosis in 
      chronic asthmatic mice. METHODS: A mouse model of chronic asthma was established. 
      Ovalbumin was used to induce chronic asthma in the mice. The levels of 
      transforming growth factor beta (TGF-beta), interleukin (IL)-4, IL-5, IL-13 and IL-17 
      in bronchoalveolar lavage fluid in mice were measured by enzyme-linked 
      immunoassays (ELISAs). ASMCs were transfected with miR-485 mimic, miR-485 
      inhibitor and siRNA-Smurf2. The reverse transcription quantitative polymerase 
      chain reaction (RT-qPCR) and western blot analyses were applied to detect the 
      mRNA and protein levels of Smurf2, alpha-SMA, TGF-beta1 and decapentaplegic homolog 
      (Smads). The MTT assay was utilized for cell proliferation, while flow cytometry 
      was conducted to assess cell cycle distribution and apoptosis. RESULTS: Lower 
      expression of miR-485 and higher expression levels of TGF-beta1, IL-4, IL-5, IL-13 
      and IL-17 were detected in mice with chronic asthma. Smurf2 was identified as the 
      target gene of miR-485. Upregulation of miR-485 mimic and downregulation of 
      Smurf2 decreased expression levels of Smurf2, alpha-SMA, TGF-beta1 and Smad3, inhibited 
      cell proliferation and increased apoptosis, while contrary results were observed 
      in ASMCs transfected with miR-485 inhibitor. CONCLUSION: Overexpressed miR-485 
      inhibits cell proliferation and promotes apoptosis of ASMCs through the 
      Smurf2-mediated TGF-beta/Smads signaling pathway in mice with chronic asthma.
CI  - (c) 2018 The Author(s). Published by S. Karger AG, Basel.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Respiratory Medicine, China-Japan Union Hospital of Jilin 
      University, Changchun, China.
FAU - Li, Hong-Yan
AU  - Li HY
AD  - Department of Infections, China-Japan Union Hospital of Jilin University, 
      Changchun, China.
FAU - Wang, Hu-Shan
AU  - Wang HS
AD  - Department of Anesthesiology, The First Hospital of Jilin University, Changchun, 
      China.
FAU - Su, Zhen-Bo
AU  - Su ZB
AD  - Department of Anesthesiology, China-Japan Union Hospital of Jilin University, 
      Changchun, Chinasuzb@jlu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20181121
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Antagomirs)
RN  - 0 (Cytokines)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Smad Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - EC 2.3.2.26 (Smurf2 protein, mouse)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
RIN - Cell Physiol Biochem. 2021;55(5):658. PMID: 34727432
MH  - 3' Untranslated Regions
MH  - Animals
MH  - Antagomirs/metabolism
MH  - Apoptosis
MH  - Asthma/metabolism/pathology/veterinary
MH  - Cell Proliferation
MH  - Chronic Disease
MH  - Cytokines/metabolism
MH  - Female
MH  - G1 Phase Cell Cycle Checkpoints
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/antagonists & inhibitors/genetics/*metabolism
MH  - Myocytes, Smooth Muscle/cytology/metabolism
MH  - RNA Interference
MH  - RNA, Small Interfering/metabolism
MH  - Signal Transduction
MH  - Smad Proteins/metabolism
MH  - Transforming Growth Factor beta/metabolism
MH  - Ubiquitin-Protein Ligases/antagonists & inhibitors/genetics/*metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Chronic asthma
OT  - MicroRNA-485
OT  - Proliferation
OT  - Smurf2
OT  - TGF-beta/Smads signaling pathway
EDAT- 2018/11/22 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/11/22 06:00
PHST- 2017/12/14 00:00 [received]
PHST- 2018/11/13 00:00 [accepted]
PHST- 2018/11/22 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/11/22 06:00 [entrez]
AID - 000495327 [pii]
AID - 10.1159/000495327 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2018;51(2):692-710. doi: 10.1159/000495327. Epub 2018 Nov 
      21.