PMID- 30467706
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230928
IS  - 2196-5404 (Electronic)
IS  - 2196-5404 (Linking)
VI  - 5
IP  - 1
DP  - 2018 Sep 25
TI  - Biological tissue sample preparation for time-of-flight secondary ion mass 
      spectrometry (ToF-SIMS) imaging.
PG  - 24
LID - 10.1186/s40580-018-0157-y [doi]
LID - 24
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging is an 
      analytical technique rapidly expanding in use in biological studies. This 
      technique is based on high spatial resolution (50-100 nm), high surface 
      sensitivity (1-2 nm top-layer), and statistical analytic power. In mass 
      spectrometry imaging (MSI), sample preparation is a crucial step to maintaining 
      the natural state of the biomolecules and providing accurate spatial information. 
      However, a number of problems associated with temperature changes in tissue 
      samples such as loss of original distribution due to undesired molecular 
      migration during the sample preparation or reduced ionization efficiency make it 
      difficult to accurately perform MSI. Although frozen hydrate analysis is the 
      ideal sample preparation method to eliminate the effects of temperature, this 
      approach is hindered by mechanical limitations. Alternatively, an 
      adhesive-tape-supported mounting and freeze-drying preparation has been proposed. 
      This paper provides a concise review of the sample preparation procedures, a 
      review of current issues, and proposes efficacious solutions for ToF-SIMS imaging 
      in biological research.
FAU - Yoon, Sohee
AU  - Yoon S
AD  - Center for Nano-Bio Measurement, Korea Research Institute of Standards and 
      Science (KRISS), Daejeon, 34113, Republic of Korea.
FAU - Lee, Tae Geol
AU  - Lee TG
AUID- ORCID: 0000-0002-6674-4147
AD  - Center for Nano-Bio Measurement, Korea Research Institute of Standards and 
      Science (KRISS), Daejeon, 34113, Republic of Korea. tglee@kriss.re.kr.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20180925
PL  - England
TA  - Nano Converg
JT  - Nano convergence
JID - 101695675
PMC - PMC6153193
OTO - NOTNLM
OT  - Adhesive tape mounting
OT  - Analyte migration
OT  - Cholesterol
OT  - Freeze drying
OT  - Frozen hydrate analysis
OT  - Sample preparation
OT  - Thaw mounting
OT  - Tissue section
OT  - ToF-SIMS imaging
EDAT- 2018/11/24 06:00
MHDA- 2018/11/24 06:01
PMCR- 2018/09/25
CRDT- 2018/11/24 06:00
PHST- 2018/08/09 00:00 [received]
PHST- 2018/09/05 00:00 [accepted]
PHST- 2018/11/24 06:00 [entrez]
PHST- 2018/11/24 06:00 [pubmed]
PHST- 2018/11/24 06:01 [medline]
PHST- 2018/09/25 00:00 [pmc-release]
AID - 10.1186/s40580-018-0157-y [pii]
AID - 157 [pii]
AID - 10.1186/s40580-018-0157-y [doi]
PST - epublish
SO  - Nano Converg. 2018 Sep 25;5(1):24. doi: 10.1186/s40580-018-0157-y.