PMID- 30468451
OWN - NLM
STAT- MEDLINE
DCOM- 20191119
LR  - 20191119
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 22
IP  - 21
DP  - 2018 Nov
TI  - LncRNA-TCL6 promotes early abortion and inhibits placenta implantation via the 
      EGFR pathway.
PG  - 7105-7112
LID - 16242 [pii]
LID - 10.26355/eurrev_201811_16242 [doi]
AB  - OBJECTIVE: The aim of this study was to investigate the role of long non-coding 
      RNA (lncRNA) TCL6 in early abortion and to explore its underlying mechanism. 
      PATIENTS AND METHODS: The expression levels of lncRNA-TCL6 and epidermal growth 
      factor receptor (EGFR) in placental tissues of normal pregnancy, threatened 
      abortion pregnancy, spontaneous abortion pregnancy, and induced abortion 
      pregnancy were detected by quantitative Real-Time Polymerase Chain Reaction 
      (qRT-PCR), respectively. Trophoblast cells were transfected with siRNA to 
      knock-down lncRNA-TCL6. Cell viability of trophoblast cells was detected by cell 
      counting kit-8 (CCK-8) assay. The protein expression levels of EGFR, 
      extracellular regulated protein kinases (ERK) and protein kinase B (AKT) in 
      trophoblast cells after lncRNA-TCL6 knockdown were detected by Western blot. 
      Rescue experiments were performed to investigate the relationship between EGFR 
      and lncRNA-TCL6. RESULTS: The expression of lncRNA-TCL6 in placenta tissues of 
      threatened abortion pregnancy was significantly higher than that of normal 
      pregnancy. Meanwhile, the expression of lncRNA-TCL6 in placenta tissues of 
      spontaneous abortion pregnancy was also markedly higher than induced abortion 
      pregnancy. However, the expression of EGFR showed an opposite trend. After 
      knockdown of lncRNA-TCL6 in trophoblast cells, the protein expression levels of 
      EGFR, ERK, and AKT were significantly increased when compared with those of the 
      control group. CCK-8 assay indicated that cell viability was remarkably increased 
      after knockdown of lncRNA-TCL6, which could be reversed by EGFR knockdown. 
      CONCLUSIONS: Compared with normal pregnancy, lncRNA-TCL6 was highly expressed in 
      placental tissues of threatened abortion pregnancy. Moreover, the expression of 
      lncRNA-TCL6 in placenta tissues of spontaneous abortion pregnancy was 
      significantly higher than induced abortion pregnancy. Knockdown of lncRNA-TCL6 
      promoted the proliferation of trophoblast cells and inhibited the abortion via 
      the EGFR signaling pathway.
FAU - Liu, L-P
AU  - Liu LP
AD  - Department of Obstetrics and Gynecology, Hanchuan People's Hospital of Hubei 
      Province, Hanchuan, China. 454586966@qq.com.
FAU - Gong, Y-B
AU  - Gong YB
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (long noncoding RNA TCL6, human)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Abortion, Induced
MH  - Abortion, Spontaneous/*genetics/metabolism
MH  - Cell Survival/genetics
MH  - Embryo Implantation/*genetics
MH  - ErbB Receptors/metabolism
MH  - Female
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Placenta/metabolism
MH  - Pregnancy
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - RNA, Long Noncoding/*genetics
MH  - Signal Transduction
MH  - Trophoblasts/metabolism
EDAT- 2018/11/24 06:00
MHDA- 2019/11/20 06:00
CRDT- 2018/11/24 06:00
PHST- 2018/11/24 06:00 [entrez]
PHST- 2018/11/24 06:00 [pubmed]
PHST- 2019/11/20 06:00 [medline]
AID - 16242 [pii]
AID - 10.26355/eurrev_201811_16242 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2018 Nov;22(21):7105-7112. doi: 
      10.26355/eurrev_201811_16242.