PMID- 3047003
OWN - NLM
STAT- MEDLINE
DCOM- 19881020
LR  - 20201209
IS  - 0016-6758 (Print)
IS  - 0016-6758 (Linking)
VI  - 24
IP  - 5
DP  - 1988 May
TI  - [The role of the RS1 sequence of Vibrio cholerae in the amplification of a 
      segment of plasmid DNA carrying the tetracycline resistance gene and cholera 
      toxin genes].
PG  - 821-8
AB  - The clones with 4 to 30-fold increased level of tetracycline resistance (TcR) 
      were selected from the strain of Escherichia coli K-12 carrying the pCO107 
      plasmid. The plasmid is the cointegrate formed from the plasmids pOX38 
      (F-derivative) and pCT105 (pBR322-derivative carrying the vct operon of Vibrio 
      cholerae eltor and the RSI sequence). pCO107 contains RSI at the junctions of two 
      plasmid genomes. Using restriction analysis and Southern blot hybridization 
      technique it was shown that increased tetracycline resistance is accompanied by 
      amplification of the pCT105 segment of pCO107 and depends upon the presence of 
      direct repeats of flanking RSI. Amplification of the pCT105 also resulted in 
      increased production of the cholerae toxin (CT).
FAU - Fil'kova, S L
AU  - Fil'kova SL
FAU - Il'ina, T S
AU  - Il'ina TS
FAU - Gintsburg, A L
AU  - Gintsburg AL
FAU - Ianishevskii, N V
AU  - Ianishevskii NV
FAU - Smirnov, G B
AU  - Smirnov GB
LA  - rus
PT  - Journal Article
TT  - Rol' RS1-posledovatel'nosti kholernogo vibriona v amplifikatsii segmenta 
      plazmidnoi DNK, nesushchego gen rezistentnosti k tetratsiklinu i geny kholernogo 
      toksina.
PL  - Russia (Federation)
TA  - Genetika
JT  - Genetika
JID - 0047354
RN  - 0 (DNA, Bacterial)
RN  - 0 (Genetic Markers)
RN  - 9012-63-9 (Cholera Toxin)
RN  - F8VB5M810T (Tetracycline)
SB  - IM
MH  - Base Sequence
MH  - Cholera Toxin/biosynthesis/*genetics
MH  - DNA, Bacterial/*genetics
MH  - Drug Resistance, Microbial/genetics
MH  - Escherichia coli/genetics
MH  - *Gene Amplification
MH  - *Genes, Bacterial
MH  - Genetic Markers
MH  - Nucleic Acid Hybridization
MH  - *Plasmids
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Tetracycline/*antagonists & inhibitors
MH  - Vibrio cholerae/*genetics/metabolism
EDAT- 1988/05/01 00:00
MHDA- 1988/05/01 00:01
CRDT- 1988/05/01 00:00
PHST- 1988/05/01 00:00 [pubmed]
PHST- 1988/05/01 00:01 [medline]
PHST- 1988/05/01 00:00 [entrez]
PST - ppublish
SO  - Genetika. 1988 May;24(5):821-8.