PMID- 30471195
OWN - NLM
STAT- MEDLINE
DCOM- 20200706
LR  - 20200706
IS  - 1462-5822 (Electronic)
IS  - 1462-5814 (Linking)
VI  - 21
IP  - 4
DP  - 2019 Apr
TI  - Chlamydia trachomatis impairs host base excision repair by downregulating 
      polymerase beta.
PG  - e12986
LID - 10.1111/cmi.12986 [doi]
AB  - Chlamydia trachomatis infections have been associated with ovarian cancer by 
      several epidemiological studies. Here, we show that C. trachomatis-infected 
      primary human ovarian epithelial cells display elevated oxidative DNA damage. 
      Base excision repair, an important cellular mechanism to repair oxidative DNA 
      lesions, was impaired in infected primary ovarian and in several other types of 
      cells. Polymerase beta was downregulated in infected cells associated with 
      upregulation of microRNA-499a (miR-499a). Stabilising polymerase beta by inhibiting 
      miR-499a significantly improved repair. Moreover, downregulation of tumour 
      suppressor p53 also resulted in attenuated repair in these cells. Thus, our data 
      show that downregulation of polymerase beta by direct inhibition through miR-499a 
      and downregulation of p53 debilitate the host-cell base excision repair during 
      C. trachomatis infection.
CI  - (c) 2018 John Wiley & Sons Ltd.
FAU - Gulve, Nitish
AU  - Gulve N
AD  - Department of Microbiology, University of Wuerzburg Biocenter, Wuerzburg, 
      Germany.
FAU - Prusty, Bhupesh K
AU  - Prusty BK
AD  - Department of Microbiology, University of Wuerzburg Biocenter, Wuerzburg, 
      Germany.
FAU - Rudel, Thomas
AU  - Rudel T
AUID- ORCID: 0000-0003-4740-6991
AD  - Department of Microbiology, University of Wuerzburg Biocenter, Wuerzburg, 
      Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190109
PL  - India
TA  - Cell Microbiol
JT  - Cellular microbiology
JID - 100883691
SB  - IM
MH  - Chlamydia Infections/*metabolism/parasitology
MH  - Chlamydia trachomatis/*pathogenicity
MH  - DNA Damage/genetics/physiology
MH  - DNA Repair/genetics/physiology
MH  - Down-Regulation
MH  - Epithelial Cells/*metabolism/*parasitology
MH  - Female
MH  - Humans
MH  - Immunoblotting
MH  - Ovary/cytology
MH  - Real-Time Polymerase Chain Reaction
EDAT- 2018/11/25 06:00
MHDA- 2020/07/07 06:00
CRDT- 2018/11/25 06:00
PHST- 2018/04/25 00:00 [received]
PHST- 2018/10/22 00:00 [revised]
PHST- 2018/11/22 00:00 [accepted]
PHST- 2018/11/25 06:00 [pubmed]
PHST- 2020/07/07 06:00 [medline]
PHST- 2018/11/25 06:00 [entrez]
AID - 10.1111/cmi.12986 [doi]
PST - ppublish
SO  - Cell Microbiol. 2019 Apr;21(4):e12986. doi: 10.1111/cmi.12986. Epub 2019 Jan 9.