PMID- 30471723
OWN - NLM
STAT- MEDLINE
DCOM- 20190114
LR  - 20200930
IS  - 1873-2259 (Electronic)
IS  - 0168-9452 (Linking)
VI  - 278
DP  - 2019 Jan
TI  - Visualization of soluble carbohydrate distribution in apple fruit flesh utilizing 
      MALDI-TOF MS imaging.
PG  - 107-112
LID - S0168-9452(18)30637-X [pii]
LID - 10.1016/j.plantsci.2018.08.014 [doi]
AB  - To confirm availability of Matrix-assisted laser desorption/ionization 
      time-of-flight (MALDI-TOF) mass spectrometry imaging (MSI) for visualizing 
      distribution of soluble carbohydrates in apple (Malus domestica) fruits a 
      horizontal fruit flesh specimen was cut from a matured 'Fuji' fruit, mounted on a 
      glass slide, lyophilized and then ion intensities of individual soluble 
      carbohydrates were probed around the specimen using a MALDI-TOF MSI apparatus 
      automatically. Contents of soluble carbohydrates in adjacent tissue of the same 
      fruit were also determined using HPLC to compare the distribution of individual 
      carbohydrate based on the ion intensities from MALDI-TOF MSI with those from 
      HPLC. Positive correlation (P <  0.001, R(2) > 0.95) was confirmed between the 
      concentration of each standard carbohydrate and the relative ion intensity of 
      MALDI-TOF mass spectrometry (MS), and thus it seems possible to use the ion 
      intensity of MALDI-TOF MS for determining the relative concentration of 
      carbohydrates in a sample. Singly charged ions attached with a potassium ion only 
      were detected from the apple fruit specimen when DHB was used as a matrix for 
      MALDI-TOF MSI. Graded increase of sucrose content from center to cortex side of 
      the fruit flesh was confirmed by both MALDI-TOF MSI and HPLC. When pseudo color 
      images on the distribution of individual carbohydrates based on the results from 
      MALDI-TOF MSI were compared with the content of carbohydrates in the adjacent 16 
      tissue blocks quantified using HPLC, strong (P <  0.001, R(2) = 0.6222) and weak 
      (P <  0.10, R(2) = 0.2123) correlation was confirmed between the brightness and 
      the content of sucrose and sorbitol, respectively. These facts indicate that 
      distribution of sucrose and sorbitol in apple fruit tissue can be visualized 
      using MALDI-TOF MSI. Thus, MALDI-TOF MSI will be useful for examining 
      carbohydrate metabolism during the maturing of apple fruit.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Horikawa, Kentaro
AU  - Horikawa K
AD  - Research Faculty and Graduate School of Agriculture, Hokkaido University, 
      Sapporo, Hokkaido 060-8589, Japan.
FAU - Hirama, Takuya
AU  - Hirama T
AD  - Research Faculty and Graduate School of Agriculture, Hokkaido University, 
      Sapporo, Hokkaido 060-8589, Japan.
FAU - Shimura, Hanako
AU  - Shimura H
AD  - Research Faculty and Graduate School of Agriculture, Hokkaido University, 
      Sapporo, Hokkaido 060-8589, Japan.
FAU - Jitsuyama, Yutaka
AU  - Jitsuyama Y
AD  - Research Faculty and Graduate School of Agriculture, Hokkaido University, 
      Sapporo, Hokkaido 060-8589, Japan.
FAU - Suzuki, Takashi
AU  - Suzuki T
AD  - Research Faculty and Graduate School of Agriculture, Hokkaido University, 
      Sapporo, Hokkaido 060-8589, Japan. Electronic address: 
      suz-tak@res.agr.hokudai.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20180826
PL  - Ireland
TA  - Plant Sci
JT  - Plant science : an international journal of experimental plant biology
JID - 9882015
RN  - 0 (Carbohydrates)
RN  - 506T60A25R (Sorbitol)
RN  - 57-50-1 (Sucrose)
SB  - IM
MH  - Carbohydrates/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Fruit/metabolism
MH  - Malus/*metabolism
MH  - Sorbitol/analysis/metabolism
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
MH  - Sucrose/analysis/metabolism
OTO - NOTNLM
OT  - Fruit flesh
OT  - HPLC
OT  - MALDI-TOF MSI
OT  - Malus domestica
OT  - Receptacle
OT  - Soluble carbohydrates
EDAT- 2018/11/26 06:00
MHDA- 2019/01/15 06:00
CRDT- 2018/11/26 06:00
PHST- 2018/06/04 00:00 [received]
PHST- 2018/08/21 00:00 [revised]
PHST- 2018/08/23 00:00 [accepted]
PHST- 2018/11/26 06:00 [entrez]
PHST- 2018/11/26 06:00 [pubmed]
PHST- 2019/01/15 06:00 [medline]
AID - S0168-9452(18)30637-X [pii]
AID - 10.1016/j.plantsci.2018.08.014 [doi]
PST - ppublish
SO  - Plant Sci. 2019 Jan;278:107-112. doi: 10.1016/j.plantsci.2018.08.014. Epub 2018 
      Aug 26.