PMID- 30471861
OWN - NLM
STAT- MEDLINE
DCOM- 20190603
LR  - 20190603
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 508
IP  - 1
DP  - 2019 Jan 1
TI  - Identification of WWP1 as an obesity-associated E3 ubiquitin ligase with a 
      protective role against oxidative stress in adipocytes.
PG  - 117-122
LID - S0006-291X(18)32560-9 [pii]
LID - 10.1016/j.bbrc.2018.11.127 [doi]
AB  - White adipose tissue (WAT) is not only the main tissue for energy storage but 
      also an endocrine organ that secretes adipokines. Obesity is the most common 
      metabolic disorder and is related to alterations in WAT characteristics, such as 
      chronic inflammation and increasing oxidative stress. WW domain containing E3 
      ubiquitin protein ligase 1 (WWP1) is a HECT-type ubiquitin E3 ligase that has 
      been implicated in various pathologies. In the present study, we found that WWP1 
      was upregulated in obese WAT in a p53-dependent manner. To investigate the 
      functions of WWP1 in adipocytes, a proteome analysis of WWP1 overexpression (OE) 
      and knockdown (KD) 3T3-L1 cells was performed. This analysis showed a positive 
      correlation between WWP1 expression and the abundance of several antioxidative 
      proteins. Thus, we measured reactive oxygen species (ROS) in WWP1 OE and KD 
      cells. Consistent with the proteome results, WWP1 OE reduced ROS levels, whereas 
      KD increased them. These findings indicate that WWP1 is an obesity-inducible E3 
      ubiquitin ligase that can protect against obesity-associated oxidative stress in 
      WAT.
CI  - Copyright (c) 2018 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Kobayashi, Masaki
AU  - Kobayashi M
AD  - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical 
      Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan; Translational Research Center, Research Institute of Science and 
      Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan.
FAU - Hoshino, Shunsuke
AU  - Hoshino S
AD  - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical 
      Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan; Translational Research Center, Research Institute of Science and 
      Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan.
FAU - Abe, Takuro
AU  - Abe T
AD  - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical 
      Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan.
FAU - Okita, Naoyuki
AU  - Okita N
AD  - Translational Research Center, Research Institute of Science and Technology, 
      Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan; 
      Division of Pathological Biochemistry, Faculty of Pharmaceutical Sciences, 
      Sanyo-Onoda City University, 1-1-1 Daigakudori, Sanyo-onoda, Yamaguchi, 756-0884, 
      Japan.
FAU - Tagawa, Ryoma
AU  - Tagawa R
AD  - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical 
      Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan.
FAU - Nagai, Wataru
AU  - Nagai W
AD  - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical 
      Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan.
FAU - Konno, Ryutaro
AU  - Konno R
AD  - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical 
      Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan.
FAU - Suzuki, Yuki
AU  - Suzuki Y
AD  - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical 
      Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan.
FAU - Furuya, Kazuhiro
AU  - Furuya K
AD  - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical 
      Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan.
FAU - Ishikawa, Natsumi
AU  - Ishikawa N
AD  - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical 
      Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan.
FAU - Okado, Hitoshi
AU  - Okado H
AD  - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical 
      Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan.
FAU - Oku, Misako
AU  - Oku M
AD  - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical 
      Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan.
FAU - Iwamoto, Machiko
AU  - Iwamoto M
AD  - Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo, 
      173-0015, Japan.
FAU - Miura, Yuri
AU  - Miura Y
AD  - Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo, 
      173-0015, Japan.
FAU - Sudo, Yuka
AU  - Sudo Y
AD  - Translational Research Center, Research Institute of Science and Technology, 
      Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
FAU - Higami, Yoshikazu
AU  - Higami Y
AD  - Laboratory of Molecular Pathology & Metabolic Disease, Faculty of Pharmaceutical 
      Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan; Translational Research Center, Research Institute of Science and 
      Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, 
      Japan. Electronic address: higami@rs.noda.tus.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181122
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Proteome)
RN  - 0 (RNA, Messenger)
RN  - EC 2.3.2.26 (WWP1 protein, mouse)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*metabolism
MH  - Adipose Tissue, White/metabolism
MH  - Animals
MH  - Diet, High-Fat/adverse effects
MH  - Disease Models, Animal
MH  - Gene Knockdown Techniques
MH  - Genes, p53
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Obesity/etiology/genetics/metabolism
MH  - Oxidative Stress
MH  - Proteome/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Ubiquitin-Protein Ligases/antagonists & inhibitors/genetics/*metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - Obesity
OT  - Oxidative stress
OT  - WWP1
OT  - White adipose tissue
EDAT- 2018/11/26 06:00
MHDA- 2019/06/04 06:00
CRDT- 2018/11/26 06:00
PHST- 2018/11/05 00:00 [received]
PHST- 2018/11/20 00:00 [accepted]
PHST- 2018/11/26 06:00 [pubmed]
PHST- 2019/06/04 06:00 [medline]
PHST- 2018/11/26 06:00 [entrez]
AID - S0006-291X(18)32560-9 [pii]
AID - 10.1016/j.bbrc.2018.11.127 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2019 Jan 1;508(1):117-122. doi: 
      10.1016/j.bbrc.2018.11.127. Epub 2018 Nov 22.