PMID- 30471902 OWN - NLM STAT- MEDLINE DCOM- 20190313 LR - 20190313 IS - 1525-3198 (Electronic) IS - 0022-0302 (Linking) VI - 102 IP - 2 DP - 2019 Feb TI - Expression patterns of hepatic genes involved in lipid metabolism in cows with subclinical or clinical ketosis. PG - 1725-1735 LID - S0022-0302(18)31079-8 [pii] LID - 10.3168/jds.2018-14965 [doi] AB - Dairy cows with ketosis are characterized by high blood concentrations of ketone bodies and hepatic lipid metabolism disorder. The discrepancies in the abundance of mRNA encoding a variety of hepatic candidate genes in varying degrees of ketotic cows represent specific responses of the liver to the challenge of fatty acids and ketone bodies. Importantly, the expression disorder of hepatic genes involved in lipid metabolism plays a promoting role in the onset and progression of ketosis. Thus, the aim of this study was to investigate the expression patterns of genes involved in the hepatic fatty acids uptake, transport, activation, beta-oxidation, synthesis, and esterification in the cows with subclinical ketosis (SCK) or clinical ketosis (CK). Twenty-four cows were selected into control [n = 8, beta-hydroxybutyrate (BHB) BHB >/= 1.2 mM), and CK (n = 8, BHB >/=3.0 mM) groups according to the blood BHB concentration and clinical symptoms. The accumulation of hepatic lipid, as indicated by triglycerides (TG) contents and Oil Red O and hematoxylin and eosin staining, was pronouncedly exacerbated in the tCK group compared with the control and SCK groups. The hepatic mRNA expression of fatty acids transport and activation genes, liver fatty acid-binding protein (FABP1) and long-chain acyl-CoA synthetase 1 (ACSL1), were both significantly higher in the SCK and CK groups than in the control group. The expression levels of peroxisome proliferator-activated receptor alpha (PPARA) and its target genes, carnitine palmitoyltransferase 1A (CPT1A) and carnitine palmitoyltransferase 2 (CPT2), were significantly elevated in the SCK group but reduced in the CK group compared with control group. Furthermore, the gene expression level of sterol regulatory element-binding protein 1 (SREBP1) and the protein expression level of sterol regulatory element-binding protein 1c and its target genes acetyl-CoA carboxylase 1 (ACC1), fatty acid synthase (FAS), and stearoyl-CoA desaturase-1 (SCD1) and TG synthesis genes diacylglycerol acyltransferase 1 (DGAT1) and diacylglycerol acyltransferase 2 (DGAT2) were significantly higher in the CK group relative to the control group. In short, the present data indicated that hepatic fatty acids uptake, transport, and activation are significantly increased in cows with SCK and CK, hepatic fatty acids beta-oxidation is significantly increased in SCK cows but markedly decreased in CK cows, and hepatic fatty acids and TG synthesis are significantly increased in CK cows, thereby inducing hepatic steatosis in CK cows. CI - Copyright (c) 2019 American Dairy Science Association. Published by Elsevier Inc. All rights reserved. FAU - Zhu, Yiwei AU - Zhu Y AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China. FAU - Liu, Guowen AU - Liu G AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China. FAU - Du, Xiliang AU - Du X AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China. FAU - Shi, Zhen AU - Shi Z AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China. FAU - Jin, Meiyu AU - Jin M AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China. FAU - Sha, Xueying AU - Sha X AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China. FAU - Li, Xiaobing AU - Li X AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China. FAU - Wang, Zhe AU - Wang Z AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China. FAU - Li, Xinwei AU - Li X AD - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, Jilin, China. Electronic address: lixinwei100@126.com. LA - eng PT - Journal Article DEP - 20181122 PL - United States TA - J Dairy Sci JT - Journal of dairy science JID - 2985126R RN - 0 (RNA, Messenger) SB - IM MH - Animals MH - Cattle MH - Cattle Diseases/*metabolism MH - Fatty Liver MH - Female MH - Ketosis/metabolism/*veterinary MH - Lipid Metabolism/*physiology MH - Liver/*metabolism MH - RNA, Messenger/metabolism OTO - NOTNLM OT - fatty acids OT - ketosis OT - lipid metabolism OT - liver EDAT- 2018/11/26 06:00 MHDA- 2019/03/14 06:00 CRDT- 2018/11/26 06:00 PHST- 2018/04/23 00:00 [received] PHST- 2018/09/29 00:00 [accepted] PHST- 2018/11/26 06:00 [pubmed] PHST- 2019/03/14 06:00 [medline] PHST- 2018/11/26 06:00 [entrez] AID - S0022-0302(18)31079-8 [pii] AID - 10.3168/jds.2018-14965 [doi] PST - ppublish SO - J Dairy Sci. 2019 Feb;102(2):1725-1735. doi: 10.3168/jds.2018-14965. Epub 2018 Nov 22.