PMID- 30474052
OWN - NLM
STAT- MEDLINE
DCOM- 20190930
LR  - 20190930
IS  - 2375-2548 (Electronic)
IS  - 2375-2548 (Linking)
VI  - 4
IP  - 11
DP  - 2018 Nov
TI  - Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors 
      for early diagnosis of HIV infection.
PG  - eaar6280
LID - 10.1126/sciadv.aar6280 [doi]
LID - eaar6280
AB  - We have engineered streptavidin-labeled fluorescent gold nanoclusters to develop 
      a gold nanocluster immunoassay (GNCIA) for the early and sensitive detection of 
      HIV infection. We performed computational simulations on the mechanism of 
      interaction between the nanoclusters and the streptavidin protein via in silico 
      studies and showed that gold nanoclusters enhance the binding to the protein, by 
      enhancing interaction between the Au atoms and the specific active site residues, 
      compared to other metal nanoclusters. We also evaluated the role of glutathione 
      conjugation in binding to gold nanoclusters with streptavidin. As proof of 
      concept, GNCIA achieved a sensitivity limit of detection of HIV-1 p24 antigen in 
      clinical specimens of 5 pg/ml, with a detection range up to1000 pg/ml in a linear 
      dose-dependent manner. GNCIA demonstrated a threefold higher sensitivity and 
      specificity compared to enzyme-linked immunosorbent assay for the detection of 
      HIV p24 antigen. The specificity of the immunoassay was 100% when tested with 
      plasma samples negative for HIV-1 p24 antigen and positive for viruses such as 
      hepatitis B virus, hepatitis C virus, and dengue. GNCIA could be developed into a 
      universal labeling technology using the relevant capture and detector antibodies 
      for the specific detection of antigens of various pathogens in the future.
FAU - Kurdekar, Aditya Dileep
AU  - Kurdekar AD
AD  - Laboratories for Nanoscience and Nanotechnology Research, Department of Physics, 
      Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam, Andhra Pradesh, 
      India.
FAU - Avinash Chunduri, L A
AU  - Avinash Chunduri LA
AD  - Andhra Med Tech Zone, Hill No. 2, IT Park Madhurwada, Rushikonda, Vishakhapatnam, 
      Andhra Pradesh 530045, India.
FAU - Manohar, C Sai
AU  - Manohar CS
AUID- ORCID: 0000-0001-6270-9574
AD  - Department of Chemistry, Sri Sathya Sai Institute of Higher Learning, 
      Prasanthinilayam, India.
FAU - Haleyurgirisetty, Mohan Kumar
AU  - Haleyurgirisetty MK
AUID- ORCID: 0000-0001-6423-1420
AD  - Laboratory of Molecular Virology, Center for Biologics Evaluation and Research 
      (CBER), Food and Drug Administration, Silver Spring, MD 20993, USA.
FAU - Hewlett, Indira K
AU  - Hewlett IK
AD  - Laboratory of Molecular Virology, Center for Biologics Evaluation and Research 
      (CBER), Food and Drug Administration, Silver Spring, MD 20993, USA.
FAU - Venkataramaniah, Kamisetti
AU  - Venkataramaniah K
AD  - Laboratories for Nanoscience and Nanotechnology Research, Department of Physics, 
      Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam, Andhra Pradesh, 
      India.
LA  - eng
PT  - Journal Article
DEP - 20181121
PL  - United States
TA  - Sci Adv
JT  - Science advances
JID - 101653440
RN  - 0 (HIV Core Protein p24)
RN  - 7440-57-5 (Gold)
RN  - 9013-20-1 (Streptavidin)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Case-Control Studies
MH  - Early Diagnosis
MH  - *Fluorescence
MH  - Gold/*chemistry
MH  - HIV/immunology
MH  - HIV Core Protein p24/*blood/immunology
MH  - HIV Infections/blood/*diagnosis/immunology
MH  - Humans
MH  - Metal Nanoparticles/*chemistry
MH  - Streptavidin/*chemistry
PMC - PMC6248912
EDAT- 2018/11/27 06:00
MHDA- 2019/10/01 06:00
PMCR- 2018/11/21
CRDT- 2018/11/27 06:00
PHST- 2017/11/30 00:00 [received]
PHST- 2018/10/25 00:00 [accepted]
PHST- 2018/11/27 06:00 [entrez]
PHST- 2018/11/27 06:00 [pubmed]
PHST- 2019/10/01 06:00 [medline]
PHST- 2018/11/21 00:00 [pmc-release]
AID - aar6280 [pii]
AID - 10.1126/sciadv.aar6280 [doi]
PST - epublish
SO  - Sci Adv. 2018 Nov 21;4(11):eaar6280. doi: 10.1126/sciadv.aar6280. eCollection 
      2018 Nov.