PMID- 30477219 OWN - NLM STAT- MEDLINE DCOM- 20190129 LR - 20200225 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 23 IP - 12 DP - 2018 Nov 24 TI - Effects of Kisspeptin-10 on Hypothalamic Neuropeptides and Neurotransmitters Involved in Appetite Control. LID - 10.3390/molecules23123071 [doi] LID - 3071 AB - Besides its role as key regulator in gonadotropin releasing hormone secretion, reproductive function, and puberty onset, kisspeptin has been proposed to act as a bridge between energy homeostasis and reproduction. In the present study, to characterize the role of hypothalamic kisspeptin as metabolic regulator, we evaluated the effects of kisspeptin-10 on neuropeptide Y (NPY) and brain-derived neurotrophic factor (BDNF) gene expression and the extracellular dopamine (DA), norepinephrine (NE), serotonin (5-hydroxytriptamine, 5-HT), dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIIA) concentrations in rat hypothalamic (Hypo-E22) cells. Our study showed that kisspeptin-10 in the concentration range 1 nM(-)10 muM was well tolerated by the Hypo-E22 cell line. Moreover, kisspeptin-10 (100 nM(-)10 muM) concentration independently increased the gene expression of NPY while BDNF was inhibited only at the concentration of 10 muM. Finally, kisspeptin-10 decreased 5-HT and DA, leaving unaffected NE levels. The inhibitory effect on DA and 5-HT is consistent with the increased peptide-induced DOPAC/DA and 5-HIIA/5-HT ratios. In conclusion, our current findings suggesting the increased NPY together with decreased BDNF and 5-HT activity following kisspeptin-10 would be consistent with a possible orexigenic effect induced by the peptide. FAU - Orlando, Giustino AU - Orlando G AD - Department of Pharmacy, "G. d'Annunzio" University, Via dei Vestini 31, 66100 Chieti, Italy. giustino.orlando@unich.it. FAU - Leone, Sheila AU - Leone S AD - Department of Pharmacy, "G. d'Annunzio" University, Via dei Vestini 31, 66100 Chieti, Italy. sheila.leone@unich.it. FAU - Ferrante, Claudio AU - Ferrante C AUID- ORCID: 0000-0001-9431-9407 AD - Department of Pharmacy, "G. d'Annunzio" University, Via dei Vestini 31, 66100 Chieti, Italy. claudio.ferrante@unich.it. FAU - Chiavaroli, Annalisa AU - Chiavaroli A AD - Department of Pharmacy, "G. d'Annunzio" University, Via dei Vestini 31, 66100 Chieti, Italy. annalisa.chiavaroli@unich.it. FAU - Mollica, Adriano AU - Mollica A AD - Department of Pharmacy, "G. d'Annunzio" University, Via dei Vestini 31, 66100 Chieti, Italy. adriano.mollica@unich.it. FAU - Stefanucci, Azzurra AU - Stefanucci A AD - Department of Pharmacy, "G. d'Annunzio" University, Via dei Vestini 31, 66100 Chieti, Italy. azzurra.stefanucci@unich.it. FAU - Macedonio, Giorgia AU - Macedonio G AUID- ORCID: 0000-0002-6592-2191 AD - Department of Pharmacy, "G. d'Annunzio" University, Via dei Vestini 31, 66100 Chieti, Italy. giorgia.macedonio@unich.it. FAU - Dimmito, Marilisa Pia AU - Dimmito MP AD - Department of Pharmacy, "G. d'Annunzio" University, Via dei Vestini 31, 66100 Chieti, Italy. marilisa.dimmito@unich.it. FAU - Leporini, Lidia AU - Leporini L AD - Department of Pharmacy, "G. d'Annunzio" University, Via dei Vestini 31, 66100 Chieti, Italy. lidia.leporini@unich.it. FAU - Menghini, Luigi AU - Menghini L AUID- ORCID: 0000-0002-7346-7395 AD - Department of Pharmacy, "G. d'Annunzio" University, Via dei Vestini 31, 66100 Chieti, Italy. luigi.menghini@unich.it. FAU - Brunetti, Luigi AU - Brunetti L AD - Department of Pharmacy, "G. d'Annunzio" University, Via dei Vestini 31, 66100 Chieti, Italy. luigi.brunetti@unich.it. FAU - Recinella, Lucia AU - Recinella L AD - Department of Pharmacy, "G. d'Annunzio" University, Via dei Vestini 31, 66100 Chieti, Italy. lucia.recinella@unich.it. LA - eng PT - Journal Article DEP - 20181124 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (KISS1 protein, human) RN - 0 (Kisspeptins) RN - 0 (Neuropeptides) RN - 0 (Neurotransmitter Agents) SB - IM MH - Appetite/*drug effects MH - Cell Line MH - Chromatography, High Pressure Liquid MH - Hypothalamus/*metabolism MH - Kisspeptins/chemistry/*pharmacology MH - Neuropeptides/chemistry/*pharmacology MH - Neurotransmitter Agents/chemistry/*pharmacology PMC - PMC6321454 OTO - NOTNLM OT - 5-hydroxytriptamine OT - brain-derived neurotrophic factor OT - dopamine OT - kisspeptin-10 OT - neuropeptide Y COIS- The authors declare no conflict of interest. EDAT- 2018/11/28 06:00 MHDA- 2019/01/30 06:00 PMCR- 2018/11/24 CRDT- 2018/11/28 06:00 PHST- 2018/10/31 00:00 [received] PHST- 2018/11/15 00:00 [revised] PHST- 2018/11/20 00:00 [accepted] PHST- 2018/11/28 06:00 [entrez] PHST- 2018/11/28 06:00 [pubmed] PHST- 2019/01/30 06:00 [medline] PHST- 2018/11/24 00:00 [pmc-release] AID - molecules23123071 [pii] AID - molecules-23-03071 [pii] AID - 10.3390/molecules23123071 [doi] PST - epublish SO - Molecules. 2018 Nov 24;23(12):3071. doi: 10.3390/molecules23123071.