PMID- 30478009 OWN - NLM STAT- MEDLINE DCOM- 20190404 LR - 20190404 IS - 1877-9603 (Electronic) IS - 1877-959X (Linking) VI - 10 IP - 2 DP - 2019 Feb TI - Infection of newly identified phleboviruses in ticks and wild animals in Hokkaido, Japan indicating tick-borne life cycles. PG - 328-335 LID - S1877-959X(18)30283-8 [pii] LID - 10.1016/j.ttbdis.2018.11.012 [doi] AB - Recent discoveries of tick-borne pathogens have raised public health concerns on tick-borne infectious diseases and emphasize the need to assess potential risks of unrecognized tick-borne pathogens. First, to determine the existence of tick-borne phleboviruses (TBPVs), genetic surveillance of phleboviruses in ticks was conducted mainly in Hokkaido, the northernmost island in Japan from 2013 to 2015. Genes of two TBPVs, previously reported as Mukawa virus (MKWV) and a newly identified relative of MKWV, Kuriyama virus (KURV), were detected and the viruses were isolated from Ixodes persulcatus collected in Hokkaido, but not in I. persulcatus collected from other areas of Japan. These viruses were phylogenetically and antigenically similar to each other. Next, to investigate the infection of MKWV in mammals, serum samples from wildlife captured in Hokkaido from 2007 to 2011 were used for serological screening. Neutralizing antibodies against MKWV were detected in both Yezo-deer (Cervus nippon yesoensis) (2/50) and raccoons (Procyon lotor) (16/64). However, no infectious MKWV was recovered from laboratory mice in experimental infections, though viral RNAs were detected in their tissues. Thus, MKWV and KURV may maintain tick-mammalian life cycles in Hokkaido, suggesting their potential as causative agents of tick-borne diseases in mammals. CI - Copyright (c) 2018 Elsevier GmbH. All rights reserved. FAU - Torii, Shiho AU - Torii S AD - Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan; Laboratory of Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan. FAU - Matsuno, Keita AU - Matsuno K AD - Laboratory of Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan; Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan. Electronic address: matsuno@vetmed.hokudai.ac.jp. FAU - Qiu, Yongjin AU - Qiu Y AD - Division of Collaboration and Education, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan. FAU - Mori-Kajihara, Akina AU - Mori-Kajihara A AD - Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan. FAU - Kajihara, Masahiro AU - Kajihara M AD - Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan. FAU - Nakao, Ryo AU - Nakao R AD - Laboratory of Parasitology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan. FAU - Nao, Naganori AU - Nao N AD - Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan. FAU - Okazaki, Katsunori AU - Okazaki K AD - Laboratory of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Tobetsu, Japan. FAU - Sashika, Mariko AU - Sashika M AD - Laboratory of Wildlife Biology and Medicine, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan. FAU - Hiono, Takahiro AU - Hiono T AD - Laboratory of Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan. FAU - Okamatsu, Masatoshi AU - Okamatsu M AD - Laboratory of Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan. FAU - Sakoda, Yoshihiro AU - Sakoda Y AD - Laboratory of Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan; Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan. FAU - Ebihara, Hideki AU - Ebihara H AD - Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA. FAU - Takada, Ayato AU - Takada A AD - Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan; Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan; Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia. FAU - Sawa, Hirofumi AU - Sawa H AD - Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan; Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan; Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia; Global Virus Network, Baltimore, USA. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20181116 PL - Netherlands TA - Ticks Tick Borne Dis JT - Ticks and tick-borne diseases JID - 101522599 RN - 0 (Antibodies, Neutralizing) RN - 0 (Antibodies, Viral) RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Animals, Wild/*virology MH - Antibodies, Neutralizing/blood MH - Antibodies, Viral/blood MH - Bunyaviridae Infections/*epidemiology MH - Deer/virology MH - Female MH - Ixodes/*virology MH - Japan/epidemiology MH - Mice MH - Mice, Inbred C57BL MH - Phlebovirus/genetics/isolation & purification MH - Phylogeny MH - RNA, Viral MH - Raccoons/virology MH - Serologic Tests MH - Tick-Borne Diseases/blood/epidemiology OTO - NOTNLM OT - Ixodes persulcatus OT - Phlebovirus OT - Tick-borne virus EDAT- 2018/11/28 06:00 MHDA- 2019/04/05 06:00 CRDT- 2018/11/28 06:00 PHST- 2018/07/02 00:00 [received] PHST- 2018/10/31 00:00 [revised] PHST- 2018/11/12 00:00 [accepted] PHST- 2018/11/28 06:00 [pubmed] PHST- 2019/04/05 06:00 [medline] PHST- 2018/11/28 06:00 [entrez] AID - S1877-959X(18)30283-8 [pii] AID - 10.1016/j.ttbdis.2018.11.012 [doi] PST - ppublish SO - Ticks Tick Borne Dis. 2019 Feb;10(2):328-335. doi: 10.1016/j.ttbdis.2018.11.012. Epub 2018 Nov 16.