PMID- 30480372
OWN - NLM
STAT- MEDLINE
DCOM- 20190403
LR  - 20190403
IS  - 1751-553X (Electronic)
IS  - 1751-5521 (Linking)
VI  - 41
IP  - 2
DP  - 2019 Apr
TI  - Flow cytometry identification of nonhemopoietic neoplasms during routine 
      immunophenotyping.
PG  - 208-217
LID - 10.1111/ijlh.12946 [doi]
AB  - INTRODUCTION: Nonhemopoietic neoplasms (NHNs) may be encountered during routine 
      flow cytometry (FC) immunophenotyping. The clue of their presence mainly relies 
      on detection of CD45-negative (CD45-) cells with altered scatter parameters. 
      METHODS: In this study, we evaluated a monoclonal antibody combination conceived 
      to characterize the CD45- population by FC, suspected of belonging to NHNs, when 
      present. The panel included CD45 for leucocytes identification, CD326 (clones 
      BerEP4 and HEA-125) to mark epithelial cells, CD33 to identify myeloid cells, 
      CD138 to trace plasma cells and CD56 useful in the identification of 
      neuroendocrine tumours. 7AAD vital dye was used to gate out dead cells. Results 
      were correlated with cytomorphology and confirmed by histological data, if 
      available. RESULTS: Among 9422 specimens submitted for routine FC investigation, 
      47 samples that included fine-needle aspirates, bone marrow aspirates, tissue 
      biopsies and body fluids had a detectable CD45- population and a sufficient cell 
      amount to be further investigated. FC revealed the presence of CD326-positive 
      epithelial cells in 38 specimens; altered scatter parameters and variable 
      reactivity to the other antigens tested allowed to suspect NHNs in the remaining 
      nine samples. The presence of NHNs was confirmed in all cases by morphology. 
      CONCLUSIONS: The current results show that when CD45- cells with altered scatter 
      parameters were detected, cytometrists involved in leukaemia/lymphoma diagnosis 
      may require further FC investigations to rapidly identify NHNs in different 
      specimens, thus reducing the time of the immunohistochemical diagnostic workup to 
      reach a final diagnosis.
CI  - (c) 2018 John Wiley & Sons Ltd.
FAU - Stacchini, Alessandra
AU  - Stacchini A
AUID- ORCID: 0000-0002-9605-7209
AD  - Flow Cytometry Unit, Pathology Division, Citta' della Salute e della Scienza 
      Hospital, Turin, Italy.
FAU - Aliberti, Sabrina
AU  - Aliberti S
AD  - Flow Cytometry Unit, Pathology Division, Citta' della Salute e della Scienza 
      Hospital, Turin, Italy.
FAU - Demurtas, Anna
AU  - Demurtas A
AD  - Flow Cytometry Unit, Pathology Division, Citta' della Salute e della Scienza 
      Hospital, Turin, Italy.
FAU - Maletta, Francesca
AU  - Maletta F
AD  - Pathology Division, Citta' della Salute e della Scienza Hospital, Turin, Italy.
FAU - Molinaro, Luca
AU  - Molinaro L
AD  - Pathology Division, Citta' della Salute e della Scienza Hospital, Turin, Italy.
FAU - Godio, Laura
AU  - Godio L
AD  - Pathology Division, Citta' della Salute e della Scienza Hospital, Turin, Italy.
FAU - Papotti, Mauro
AU  - Papotti M
AD  - Pathology Division, Citta' della Salute e della Scienza Hospital, Turin, Italy.
AD  - University of Turin, Turin, Italy.
LA  - eng
PT  - Journal Article
DEP - 20181127
PL  - England
TA  - Int J Lab Hematol
JT  - International journal of laboratory hematology
JID - 101300213
RN  - 0 (Antigens, CD)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Antigens, CD/*blood
MH  - Female
MH  - *Flow Cytometry
MH  - Humans
MH  - *Immunophenotyping
MH  - Male
MH  - Neoplasm Proteins/*blood
MH  - Neoplasms/*blood/*diagnosis
MH  - Retrospective Studies
OTO - NOTNLM
OT  - CD138
OT  - CD326
OT  - CD56
OT  - carcinoma
OT  - epithelial cells
OT  - multicolour flow cytometry
EDAT- 2018/11/28 06:00
MHDA- 2019/04/04 06:00
CRDT- 2018/11/28 06:00
PHST- 2018/07/17 00:00 [received]
PHST- 2018/10/08 00:00 [revised]
PHST- 2018/10/16 00:00 [accepted]
PHST- 2018/11/28 06:00 [pubmed]
PHST- 2019/04/04 06:00 [medline]
PHST- 2018/11/28 06:00 [entrez]
AID - 10.1111/ijlh.12946 [doi]
PST - ppublish
SO  - Int J Lab Hematol. 2019 Apr;41(2):208-217. doi: 10.1111/ijlh.12946. Epub 2018 Nov 
      27.