PMID- 30480801
OWN - NLM
STAT- MEDLINE
DCOM- 20200413
LR  - 20211204
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 234
IP  - 6
DP  - 2019 Jun
TI  - MicroRNA-99b suppresses human cervical cancer cell activity by inhibiting the 
      PI3K/AKT/mTOR signaling pathway.
PG  - 9577-9591
LID - 10.1002/jcp.27645 [doi]
AB  - Cervical cancer is common cancer among women with high morbidity. MicroRNAs 
      (miRs) are involved in the progression and development of cervical cancer. This 
      study aimed to explore the effect of miR-99b-5p (miR-99b) on invasion and 
      migration in cervical cancer through the phosphatidylinositol 3-kinase 
      (PI3K)/protein kinase B (AKT)/mechanistic target of rapamycin (mTOR) signaling 
      pathway. The microarray-based analysis was used to screen out differentially 
      expressed miRNAs. Expression of miR-99b, PI3K, AKT, mTOR, and ribosomal protein 
      S6 kinase (p70S6K) was determined in both cervical cancer tissues and 
      paracancerous tissues. Next, alteration of miR-99b expression in cervical cancer 
      was conducted to evaluate levels of PI3K, AKT, mTOR, p70S6K matrix 
      metallopeptidase 2, epithelial cell adhesion molecule, and intercellular adhesion 
      molecule 1, as well as the effect of miR-99b on cell proliferation, invasion, 
      migration, cell cycle distribution, and apoptosis. The results demonstrated that 
      miR-99b expression was decreased and levels of PI3K, AKT, mTOR, and p70S6K were 
      elevated in cervical cancer tissues. More important, overexpressed miR-99b 
      repressed the PI3K/AKT/mTOR signaling pathway, inhibited cell proliferation, 
      invasion, and migration, blocked cell cycle entry, and promoted apoptosis in 
      cervical cancer. These results indicate that miR-99b attenuates the migration and 
      invasion of human cervical cancer cells through downregulation of the 
      PI3K/AKT/mTOR signaling pathway, which provides a therapeutic approach for 
      cervical cancer treatment.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Li, Yong-Jie
AU  - Li YJ
AUID- ORCID: 0000-0001-5483-0489
AD  - Department of Obstetrics and Gynecology, Henan Provincial People's Hospital, 
      People's Hospital of Zhengzhou University, Zhengzhou, China.
FAU - Wang, Yue
AU  - Wang Y
AD  - Department of Obstetrics and Gynecology, Henan Provincial People's Hospital, 
      People's Hospital of Zhengzhou University, Zhengzhou, China.
FAU - Wang, Yi-Ying
AU  - Wang YY
AD  - Department of Obstetrics and Gynecology, Henan Provincial People's Hospital, 
      People's Hospital of Zhengzhou University, Zhengzhou, China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20181127
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (MIRN99 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
RIN - J Cell Physiol. 2022 Mar;237(3):2008. PMID: 34287878
MH  - Adult
MH  - Aged
MH  - Apoptosis/genetics
MH  - Base Sequence
MH  - Cell Cycle
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Down-Regulation/genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - MicroRNAs/genetics/*metabolism
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - *Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Up-Regulation/genetics
MH  - Uterine Cervical Neoplasms/*genetics/*pathology
OTO - NOTNLM
OT  - PI3K/AKT/mTOR
OT  - cell invasion
OT  - cell migration
OT  - cervical cancer
OT  - microRNA-99b
OT  - signaling pathway
EDAT- 2018/11/28 06:00
MHDA- 2020/04/14 06:00
CRDT- 2018/11/28 06:00
PHST- 2018/03/22 00:00 [received]
PHST- 2018/10/02 00:00 [accepted]
PHST- 2018/11/28 06:00 [pubmed]
PHST- 2020/04/14 06:00 [medline]
PHST- 2018/11/28 06:00 [entrez]
AID - 10.1002/jcp.27645 [doi]
PST - ppublish
SO  - J Cell Physiol. 2019 Jun;234(6):9577-9591. doi: 10.1002/jcp.27645. Epub 2018 Nov 
      27.