PMID- 30481606
OWN - NLM
STAT- MEDLINE
DCOM- 20190125
LR  - 20190125
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 125
DP  - 2019 Feb
TI  - Expression of gelatinases (MMP-2 and MMP-9) and tissue inhibitors of 
      metalloproteinases (TIMP-2 and TIMP-3) in the chicken ovary in relation to 
      follicle development and atresia.
PG  - 268-276
LID - S0093-691X(18)30683-6 [pii]
LID - 10.1016/j.theriogenology.2018.11.017 [doi]
AB  - Matrix metalloproteinases (MMPs) are a family of peptidases that possess the 
      ability to break down extracellular matrix macromolecules associated with tissue 
      turnover in various physiological and pathological conditions. Their activity is 
      largely regulated by specific tissue inhibitors of MMPs (TIMPs). Information 
      concerning the role of MMPs in the chicken ovary is very limited. The aim of the 
      present study was to determine the expression and localization of selected 
      members of the MMP system in different compartments of the laying hen ovary and 
      to investigate whether their expression changes at different stages of the 
      ovulatory cycle. MMP-2 and -9 activity was also examined. Expression of MMP-2, -9 
      and tissue inhibitors of MMPs (TIMP-2 and -3) in the ovarian follicles was 
      examined 22 h and 3 h before F1 ovulation. Real-time polymerase chain reaction 
      and western blot revealed differential mRNA and protein expression of MMP-2, 
      MMP-9, TIMP-2, and TIMP-3 in the ovarian follicles: white, yellowish, small 
      yellow, the largest preovulatory (F3-F1), and white atretic. Within the ovary, 
      the relative expression of MMP and TIMP mRNA depended on follicle development, 
      the layer of follicular wall, and ovulation stage. The relatively higher 
      expression of MMP-2 and MMP-9 mRNA in the ovarian follicles 3 h compared to 22 h 
      before ovulation was found. As follicle development progressed toward ovulation, 
      elevated MMP-2 and -9 activity was noted. Atresia of white follicles was 
      accompanied by an increase in gelatinase activities. Immunohistochemistry 
      demonstrated tissue- and follicle-dependent immunoreactivity of the examined MMPs 
      and TIMPs. In summary, the results show tissue- and stage of the ovulatory 
      cycle-dependent differences in MMP and TIMP expression, as well as MMP-2 and -9 
      activity. Findings that suggest these molecules might significantly participate 
      in the complex remodeling of extracellular matrix required for follicle 
      development, ovulation, and atresia in the chicken ovary.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Hrabia, Anna
AU  - Hrabia A
AD  - Department of Animal Physiology and Endocrinology, University of Agriculture in 
      Krakow, al. Mickiewicza 24/28, 30-059, Krakow, Poland. Electronic address: 
      anna.hrabia@urk.edu.pl.
FAU - Wolak, Dominika
AU  - Wolak D
AD  - Department of Animal Physiology and Endocrinology, University of Agriculture in 
      Krakow, al. Mickiewicza 24/28, 30-059, Krakow, Poland.
FAU - Kwasniewska, Maria
AU  - Kwasniewska M
AD  - Department of Animal Physiology and Endocrinology, University of Agriculture in 
      Krakow, al. Mickiewicza 24/28, 30-059, Krakow, Poland.
FAU - Kieronska, Anna
AU  - Kieronska A
AD  - Department of Animal Physiology and Endocrinology, University of Agriculture in 
      Krakow, al. Mickiewicza 24/28, 30-059, Krakow, Poland.
FAU - Socha, Joanna K
AU  - Socha JK
AD  - Department of Animal Physiology and Endocrinology, University of Agriculture in 
      Krakow, al. Mickiewicza 24/28, 30-059, Krakow, Poland.
FAU - Sechman, Andrzej
AU  - Sechman A
AD  - Department of Animal Physiology and Endocrinology, University of Agriculture in 
      Krakow, al. Mickiewicza 24/28, 30-059, Krakow, Poland.
LA  - eng
PT  - Journal Article
DEP - 20181119
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Tissue Inhibitor of Metalloproteinase-3)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Chickens/*physiology
MH  - Female
MH  - Gene Expression Regulation
MH  - Matrix Metalloproteinase 2/*metabolism
MH  - Matrix Metalloproteinase 9/genetics/*metabolism
MH  - Ovarian Follicle/*physiology
MH  - Ovulation/physiology
MH  - Tissue Inhibitor of Metalloproteinase-2/genetics/*metabolism
MH  - Tissue Inhibitor of Metalloproteinase-3/genetics/*metabolism
OTO - NOTNLM
OT  - Chicken
OT  - MMPs
OT  - Ovary
OT  - Remodeling
OT  - TIMPs
EDAT- 2018/11/28 06:00
MHDA- 2019/01/27 06:00
CRDT- 2018/11/28 06:00
PHST- 2018/08/18 00:00 [received]
PHST- 2018/11/14 00:00 [revised]
PHST- 2018/11/18 00:00 [accepted]
PHST- 2018/11/28 06:00 [pubmed]
PHST- 2019/01/27 06:00 [medline]
PHST- 2018/11/28 06:00 [entrez]
AID - S0093-691X(18)30683-6 [pii]
AID - 10.1016/j.theriogenology.2018.11.017 [doi]
PST - ppublish
SO  - Theriogenology. 2019 Feb;125:268-276. doi: 10.1016/j.theriogenology.2018.11.017. 
      Epub 2018 Nov 19.