PMID- 30483702
OWN - NLM
STAT- MEDLINE
DCOM- 20190225
LR  - 20190225
IS  - 1006-7248 (Print)
IS  - 1006-7248 (Linking)
VI  - 27
IP  - 4
DP  - 2018 Aug
TI  - [Human umbilical cord mesenchymal stem cells co-cultured with dental pulp cells 
      in vitro and its effect on cell biological behaviors].
PG  - 365-369
AB  - PURPOSE: To investigated the effect of human umbilical cord mesenchymal stem 
      cells (hUCMSCs) and human dental pulp cells (hDPCs) on cell biological behaviors 
      by co-culture system in vitro. METHODS: hUCMSCs and hDPCs were obtained by 
      primary culture. A culture system of hUCMSCs and hDPCs induced by BMP2 was 
      established in vitro. hUCMSCs and hDPCs were co-cultured at the ratio of 1:1, 1:5 
      and 5:1. The optimum ratio of each group was selected to further experiment. The 
      formation of calcium nodule was stained by alizarin red staining at 21 day. The 
      expression of DSPP，ALP，DMP1，OCN，VEGF，HGF and Nanog gene was detected by real-time 
      quantitative PCR at 7 day and 14 day. 1:1 group and hUCMSCs, hDPCs group were 
      selected for alizarin red staining at 21 day according to PCR results. 
      Statistical analysis was performed using SPSS 21.0 software package. RESULTS: 
      Calcified nodules formation in 1:1 group was significantly higher than in hUCMSCs 
      group (P<0.05), close to that in hDPCs. qPCR showed that the mRNA expression of 
      DSPP, ALP, DMP1, OCN, VEGF and HGF in 1:1 group was significantly higher than 
      that in hUCMSCs (P<0.05); mRNA expression of Nanog in 1:1 group was significantly 
      lower than in hUCMSCs group (P<0.05). The results of alizarin red staining showed 
      that the OD value of 1:1 group was significantly higher than that of hUCMSCs 
      group (P<0.05). CONCLUSIONS: The cells can be induced to differentiate into 
      odontoblastoid-like cells and the mRNA expression of angiogenic factors was 
      stimulated by hUCMSCs co-culure wih hDPCs.
FAU - Lin, Tian
AU  - Lin T
AD  - Affiliated Stomatological Hospital of School of Guangxi Medical University. 
      Nanning 530021, Guangxi Zhuang Autonomous Region, China. E-mail:8347673@qq.com.
FAU - Zhao, Wen-Qing
AU  - Zhao WQ
FAU - Lu, Yan-Ling
AU  - Lu YL
FAU - Liu, Yu-Yin
AU  - Liu YY
FAU - Bao, Li-Rong
AU  - Bao LR
FAU - Wu, Yu
AU  - Wu Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Shanghai Kou Qiang Yi Xue
JT  - Shanghai kou qiang yi xue = Shanghai journal of stomatology
JID - 101090220
SB  - IM
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - *Dental Pulp
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - Umbilical Cord/cytology
EDAT- 2018/11/30 06:00
MHDA- 2019/02/26 06:00
CRDT- 2018/11/29 06:00
PHST- 2018/11/29 06:00 [entrez]
PHST- 2018/11/30 06:00 [pubmed]
PHST- 2019/02/26 06:00 [medline]
PST - ppublish
SO  - Shanghai Kou Qiang Yi Xue. 2018 Aug;27(4):365-369.