PMID- 30486863 OWN - NLM STAT- MEDLINE DCOM- 20190415 LR - 20231004 IS - 1757-6512 (Electronic) IS - 1757-6512 (Linking) VI - 9 IP - 1 DP - 2018 Nov 28 TI - Development of a micro-tissue-mediated injectable bone tissue engineering strategy for large segmental bone defect treatment. PG - 331 LID - 10.1186/s13287-018-1064-1 [doi] LID - 331 AB - BACKGROUND: Bone tissue engineering is not widely used in clinical treatment. Two main reasons hide behind this: (1) the seed cells are difficult to obtain and (2) the process of tissue engineering bone construction is too complex and its efficiency is still relatively low. It is foreseeable that in the near future, the problem of seed cell sources could be solved completely in tissue engineering bone repair. As for the complex process and low efficiency of tissue engineering bone construction, usually two strategies would be considered: (1) the construction strategy based on injectable bone tissue and (2) the construction strategy based on osteogenic cell sheets. However, the application of injectable bone tissue engineering (iBTE) strategy and osteogenic cell sheet strategy is limited and they could hardly be used directly in repairing defects of large segmental bone, especially load-bearing bone. METHODS: In this study, we built an osteogenic micro-tissue with simple construction but with a certain structure and composition. Based on this, we established a new iBTE repair strategy-osteogenic micro-tissue in situ repair strategy, mainly targeting at solving the problem of large segmental bone defect. The steps are as follows: (1) Build the biodegradable three-dimensional scaffold based on the size of the defect site with 3D printing rapid prototyping technology. (2) Implant the three-dimensional scaffold into the defect site. This scaffold is considered as the "steel framework" that could provide both mechanical support and space for bone tissue growth. (3) Inject the osteogenic micro-tissue (i.e., the "cell-extracellular matrix" complex), which could be considered as "concrete," into the three-dimensional scaffold, to promote the bone tissue regeneration in situ. Meanwhile, the digested cells were injected as the compared group in this experiment. After 3 months, the effect of in situ bone defect repair of osteogenic micro-tissue and digested cells was compared. RESULTS: It is confirmed that osteogenic micro-tissue could achieve a higher efficiency on cell usage and has a better repair effect than the digested cells. CONCLUSIONS: Osteogenic micro-tissue repairing strategy would be a more promising clinical strategy to solve the problem of large segmental bone defect. FAU - Wu, Dingyu AU - Wu D AD - Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Key Laboratory of Tissue Engineering, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China. FAU - Wang, Zhenxing AU - Wang Z AD - Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. FAU - Wang, Jinbing AU - Wang J AD - Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Key Laboratory of Tissue Engineering, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China. FAU - Geng, Yingnan AU - Geng Y AD - Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Key Laboratory of Tissue Engineering, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China. FAU - Zhang, Zhanzhao AU - Zhang Z AD - Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Key Laboratory of Tissue Engineering, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China. FAU - Li, Yu AU - Li Y AD - Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Key Laboratory of Tissue Engineering, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China. FAU - Li, Qiannan AU - Li Q AD - Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Key Laboratory of Tissue Engineering, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China. FAU - Zheng, Zhiwei AU - Zheng Z AD - Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Key Laboratory of Tissue Engineering, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China. FAU - Cao, Yilin AU - Cao Y AD - Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Key Laboratory of Tissue Engineering, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China. yilincao@yahoo.com. AD - Translational Research Centre of Regenerative Medicine and 3D Printing Technologies of Guangzhou Medical University, The Third Affiliated Hospital of Guangzhou Medical University, No.63 Duobao Road, Liwan District, Guangzhou City, 510150, Guangdong Province, China. yilincao@yahoo.com. FAU - Zhang, Zhi-Yong AU - Zhang ZY AUID- ORCID: 0000-0003-3479-6513 AD - Translational Research Centre of Regenerative Medicine and 3D Printing Technologies of Guangzhou Medical University, The Third Affiliated Hospital of Guangzhou Medical University, No.63 Duobao Road, Liwan District, Guangzhou City, 510150, Guangdong Province, China. drzhiyong@gzhmu.edu.cn. AD - China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, 310058, China. drzhiyong@gzhmu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20181128 PL - England TA - Stem Cell Res Ther JT - Stem cell research & therapy JID - 101527581 SB - IM MH - Animals MH - Bone and Bones/diagnostic imaging/*pathology MH - *Injections MH - Osteogenesis MH - Rabbits MH - Tissue Engineering/*methods MH - Tissue Scaffolds/*chemistry MH - X-Ray Microtomography PMC - PMC6263540 OTO - NOTNLM OT - BMSCs OT - Bone regeneration OT - Large segmental bone defect OT - Micro-tissue OT - iBTE COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: The project complied with the IUCN Policy Statement on Research Involving Species at Risk of Extinction and the Convention on the Trade in Endangered Species of Wild Fauna and Flora. CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER'S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. EDAT- 2018/11/30 06:00 MHDA- 2019/04/16 06:00 PMCR- 2018/11/28 CRDT- 2018/11/30 06:00 PHST- 2018/07/31 00:00 [received] PHST- 2018/10/31 00:00 [accepted] PHST- 2018/10/18 00:00 [revised] PHST- 2018/11/30 06:00 [entrez] PHST- 2018/11/30 06:00 [pubmed] PHST- 2019/04/16 06:00 [medline] PHST- 2018/11/28 00:00 [pmc-release] AID - 10.1186/s13287-018-1064-1 [pii] AID - 1064 [pii] AID - 10.1186/s13287-018-1064-1 [doi] PST - epublish SO - Stem Cell Res Ther. 2018 Nov 28;9(1):331. doi: 10.1186/s13287-018-1064-1.