PMID- 30488386 OWN - NLM STAT- MEDLINE DCOM- 20190612 LR - 20190613 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 1906 DP - 2019 TI - Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection. PG - 79-85 LID - 10.1007/978-1-4939-8964-5_5 [doi] AB - Microchip electrophoresis is a versatile separation technique. Electrochemical detection is suitable to apply to microdevices due to its easy integration to the fabrication process and good sensitivity and selectivity. Here we describe the procedures to prepare Pt band electrodes deposited on glass to couple to polydimethylsiloxane (PDMS) microchips aiming the separation and detection of nitrite using an isolated potentiostat. FAU - Bressan, Lucas Paines AU - Bressan LP AD - Chemistry Institute, State University of Campinas, Campinas, SP, Brazil. FAU - de Jesus, Dosil Pereira AU - de Jesus DP AD - Chemistry Institute, State University of Campinas, Campinas, SP, Brazil. FAU - Gunasekara, Dulan Bandara AU - Gunasekara DB AD - Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. FAU - Lunte, Susan Marie AU - Lunte SM AD - Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, KS, USA. FAU - da Silva, Jose Alberto Fracassi AU - da Silva JAF AD - Chemistry Institute, State University of Campinas, Campinas, SP, Brazil. fracassi@iqm.unicamp.br. LA - eng PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 RN - 0 (Dimethylpolysiloxanes) RN - 0 (Nitrites) RN - 63148-62-9 (baysilon) SB - IM MH - Biosensing Techniques/instrumentation MH - Dimethylpolysiloxanes/*chemistry MH - Electrodes MH - Electrophoresis, Microchip/*instrumentation/methods MH - Glass MH - Nitrites/*analysis OTO - NOTNLM OT - Amperometric detection OT - Gated injection OT - Isolated potentiostat OT - Polydimethylsiloxane OT - Reactive nitrogen species EDAT- 2018/11/30 06:00 MHDA- 2019/06/14 06:00 CRDT- 2018/11/30 06:00 PHST- 2018/11/30 06:00 [entrez] PHST- 2018/11/30 06:00 [pubmed] PHST- 2019/06/14 06:00 [medline] AID - 10.1007/978-1-4939-8964-5_5 [doi] PST - ppublish SO - Methods Mol Biol. 2019;1906:79-85. doi: 10.1007/978-1-4939-8964-5_5.