PMID- 3049656
OWN - NLM
STAT- MEDLINE
DCOM- 19881123
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 26
IP  - 8
DP  - 1988 Aug
TI  - Comparison of immunofluorescence, enzyme immunoassay, and Western blot 
      (immunoblot) methods for detection of antibody to human T-cell leukemia virus 
      type I.
PG  - 1487-91
AB  - A total of 3,349 serum samples were screened by the immunofluorescence (IF) 
      method for antibody to human T-cell leukemia virus type I (HTLV-I). Only 9 of 
      2,409 specimens from selected individuals, blood bank donors, patients with 
      encephalitis-meningitis, and human immunodeficiency virus antibody-positive 
      homosexual or bisexual men were reactive by IF. In addition, 940 serum samples 
      from intravenous drug abusers were tested by IF and also by an HTLV-I enzyme 
      immunoassay (EIA) method. Of these, 222 (24%) were positive for both HTLV-I and 
      HTLV-II antigens by IF, and 191 of these 222 were also reactive in the HTLV-I 
      EIA. Of the 31 IF-positive, EIA-negative serum samples, 20 exhibited optical 
      density readings greater than or equal to 70% of the positive cutoff in the EIA, 
      and 29 samples reacted with 1 or more bands in the Western blot (immunoblot) 
      test. An additional 10 specimens that were EIA negative reacted only with HTLV-I 
      by IF. Differences in staining morphology and in reactions on HTLV-I and HTLV-II 
      antigens before and after absorption of the serum specimens with HTLV-I and 
      HTLV-II-infected cell pellets revealed six distinct serological patterns by IF. 
      These results indicate that infections by HTLV-I or by another closely related 
      retrovirus(es) occur in California. Further studies utilizing statistically valid 
      sampling methods are needed to estimate true prevalence rates among various 
      groups. IF and Western blot tests should supplement the EIA method to maximize 
      sensitivity and specificity of test procedures.
FAU - Gallo, D
AU  - Gallo D
AD  - Viral and Rickettsial Disease Laboratory, State of California Department of 
      Health Services, Berkeley 94704.
FAU - Hoffman, M N
AU  - Hoffman MN
FAU - Cossen, C K
AU  - Cossen CK
FAU - Diggs, J L
AU  - Diggs JL
FAU - Hurst, J W
AU  - Hurst JW
FAU - Penning, L M
AU  - Penning LM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (HTLV-I Antibodies)
SB  - IM
MH  - Blotting, Western
MH  - Fluorescent Antibody Technique
MH  - HTLV-I Antibodies/*analysis
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Predictive Value of Tests
PMC - PMC266647
EDAT- 1988/08/01 00:00
MHDA- 1988/08/01 00:01
PMCR- 1988/08/01
CRDT- 1988/08/01 00:00
PHST- 1988/08/01 00:00 [pubmed]
PHST- 1988/08/01 00:01 [medline]
PHST- 1988/08/01 00:00 [entrez]
PHST- 1988/08/01 00:00 [pmc-release]
AID - 10.1128/jcm.26.8.1487-1491.1988 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1988 Aug;26(8):1487-91. doi: 10.1128/jcm.26.8.1487-1491.1988.