PMID- 30496701
OWN - NLM
STAT- MEDLINE
DCOM- 20191112
LR  - 20191112
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 33
IP  - 3
DP  - 2019 Mar
TI  - Single-cell analyses reveal functional classification of dendritic cells and 
      their potential roles in inflammatory disease.
PG  - 3784-3794
LID - 10.1096/fj.201801489R [doi]
AB  - Dendritic cells (DCs) have crucial roles in immune-related diseases. However, it 
      is difficult to explore DCs because of their rareness and heterogeneity. Although 
      previous studies had been performed to detect the phenotypic characteristics of 
      DC populations, the functional diversity has been ignored. Using a combination of 
      flow cytometry, single-cell quantitative PCR, and bioinformatic analysis, we 
      depicted the DC panorama with not only phenotypic but also functional markers. 
      Functional classification of DCs in mouse lymphoid tissue (spleen) and 
      nonlymphoid tissue (liver) was performed. The results revealed that expression of 
      macrophage scavenger receptor 1 ( MSR1) and C-C motif chemokine receptors ( CCR) 
      1, CCR2, and CCR4 were elevated in liver DCs, suggesting increased lipid uptake 
      and migration abilities. The enriched expression of costimulatory molecule CD80, 
      TLR9, and TLR adaptor MYD88 in spleen DCs indicated a more-mature phenotype, 
      enhanced pathogen recognition, and T-cell stimulation abilities. Furthermore, we 
      compared DCs in the atherosclerotic mouse models with healthy controls. In 
      addition to the quantitative increase in DCs in the liver and spleen of the 
      apolipoprotein E-knockout ( ApoE(-/-)) mice, the functional expression patterns 
      of the DCs also changed at the single-cell level. These results promote our 
      understanding of the participation of DCs in inflammatory diseases and have 
      potential applications in DC clinical assessment.-Shi, Q., Zhuang, F., Liu, 
      J.-T., Li, N., Chen, Y.-X., Su, X.-B., Yao, A.-H., Yao, Q.-P., Han, Y., Li, 
      S.-S., Qi, Y.-X., Jiang, Z.-L. Single-cell analyses reveal functional 
      classification of dendritic cells and their potential roles in inflammatory 
      disease.
FAU - Shi, Qian
AU  - Shi Q
AD  - Institute of Mechanobiology and Medical Engineering, School of Life Sciences and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Zhuang, Fei
AU  - Zhuang F
AD  - Institute of Mechanobiology and Medical Engineering, School of Life Sciences and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Liu, Ji-Ting
AU  - Liu JT
AD  - Institute of Mechanobiology and Medical Engineering, School of Life Sciences and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Li, Na
AU  - Li N
AD  - Institute of Mechanobiology and Medical Engineering, School of Life Sciences and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Chen, Yuan-Xiu
AU  - Chen YX
AD  - Institute of Mechanobiology and Medical Engineering, School of Life Sciences and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Su, Xian-Bin
AU  - Su XB
AD  - Key Laboratory of Systems Biomedicine, Shanghai Center for Systems Biomedicine, 
      Shanghai Jiao Tong University, Shanghai, China.
FAU - Yao, Ai-Hong
AU  - Yao AH
AD  - College of Computer Science and Technology, Harbin Engineering University, 
      Harbin, China.
FAU - Yao, Qing-Ping
AU  - Yao QP
AD  - Institute of Mechanobiology and Medical Engineering, School of Life Sciences and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Han, Yue
AU  - Han Y
AD  - Institute of Mechanobiology and Medical Engineering, School of Life Sciences and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Li, Shan-Shan
AU  - Li SS
AD  - Institute of Mechanobiology and Medical Engineering, School of Life Sciences and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Qi, Ying-Xin
AU  - Qi YX
AD  - Institute of Mechanobiology and Medical Engineering, School of Life Sciences and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
FAU - Jiang, Zong-Lai
AU  - Jiang ZL
AD  - Institute of Mechanobiology and Medical Engineering, School of Life Sciences and 
      Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181129
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Receptors, CCR1)
RN  - 0 (Scavenger Receptors, Class A)
SB  - IM
MH  - Animals
MH  - Dendritic Cells/metabolism/*pathology
MH  - Flow Cytometry/methods
MH  - Inflammation/metabolism/*pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Receptors, CCR1/metabolism
MH  - Scavenger Receptors, Class A/metabolism
MH  - Single-Cell Analysis/methods
MH  - Spleen/pathology
MH  - T-Lymphocytes/metabolism/pathology
OTO - NOTNLM
OT  - antigen-presenting cell
OT  - atherosclerotic mouse models
OT  - functional heterogeneity
OT  - single-cell qPCR
EDAT- 2018/11/30 06:00
MHDA- 2019/11/13 06:00
CRDT- 2018/11/30 06:00
PHST- 2018/11/30 06:00 [pubmed]
PHST- 2019/11/13 06:00 [medline]
PHST- 2018/11/30 06:00 [entrez]
AID - 10.1096/fj.201801489R [doi]
PST - ppublish
SO  - FASEB J. 2019 Mar;33(3):3784-3794. doi: 10.1096/fj.201801489R. Epub 2018 Nov 29.