PMID- 30496821
OWN - NLM
STAT- MEDLINE
DCOM- 20190307
LR  - 20190307
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 397
DP  - 2019 Jan 15
TI  - MALAT1 lncRNA Induces Autophagy and Protects Brain Microvascular Endothelial 
      Cells Against Oxygen-Glucose Deprivation by Binding to miR-200c-3p and 
      Upregulating SIRT1 Expression.
PG  - 116-126
LID - S0306-4522(18)30757-7 [pii]
LID - 10.1016/j.neuroscience.2018.11.024 [doi]
AB  - There is growing evidence that long noncoding RNAs (lncRNAs) play important roles 
      in various biological processes. Metastasis-associated lung adenocarcinoma 
      transcript 1 (MALAT1) is one of the most highly upregulated lncRNAs in cerebral 
      ischemia. However, the molecular mechanism of MALAT1 during cerebral ischemia is 
      still unclear. This experiment is intended to investigate the role of MALAT1 in 
      cerebral ischemia and its relationship with autophagy. Oxygen-glucose deprivation 
      (OGD) in brain microvascular endothelial cells (BMECs) was used to mimic 
      ischemic-like conditions in vitro. Real-time PCR, MTT, LDH assay and western blot 
      were used to evaluate the levels of MALAT1, miR-200c-3p, SIRT1, cell survival and 
      proteins. We found that the expression of MALAT1 and LC3BII were upregulated and 
      p62 was downregulated by OGD. Inhibition of MALAT1 attenuated the autophagy 
      activation and promoted cell death. We further revealed that MALAT1 downregulated 
      the expression of miR-200c-3p by directly binding to miR-200c-3p. Furthermore, 
      miR-200c-3p inhibited the autophagy and survival in BMECs by binding to 3'UTR of 
      SIRT1, whereas MALAT1 overturned the inhibitory effect of miR-200c-3p. In 
      conclusion, our study illuminated a novel Malat1-miR-200c-3p-SIRT1 pathway in the 
      regulation of autophagy, in which, MALAT1 activates autophagy and promotes cell 
      survival by binding to miR-200c-3p and upregulating SIRT1 expression.
CI  - Copyright (c) 2018 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Wang, Shan
AU  - Wang S
AD  - Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Han, Xu
AU  - Han X
AD  - Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Mao, Zhengchun
AU  - Mao Z
AD  - Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Xin, Yanming
AU  - Xin Y
AD  - Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Maharjan, Surendra
AU  - Maharjan S
AD  - Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Zhang, Bing
AU  - Zhang B
AD  - Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China. Electronic address: zhang_bing08@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181126
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Malat1 long non-coding RNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn200 microRNA, mouse)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 3.5.1.- (Sirt1 protein, mouse)
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Autophagy/*physiology
MH  - Brain/blood supply/*metabolism
MH  - Cell Hypoxia/*physiology
MH  - Cell Line
MH  - Cell Survival/physiology
MH  - Endothelial Cells/*metabolism
MH  - Glucose/*deficiency
MH  - Mice
MH  - MicroRNAs/metabolism
MH  - Microvessels/metabolism
MH  - RNA, Long Noncoding/*metabolism
MH  - Sirtuin 1/metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - BMECs
OT  - MALAT1
OT  - MiR-200c-3p
OT  - SIRT1
OT  - autophagy
OT  - lncRNA
EDAT- 2018/11/30 06:00
MHDA- 2019/03/08 06:00
CRDT- 2018/11/30 06:00
PHST- 2018/09/15 00:00 [received]
PHST- 2018/11/11 00:00 [revised]
PHST- 2018/11/15 00:00 [accepted]
PHST- 2018/11/30 06:00 [pubmed]
PHST- 2019/03/08 06:00 [medline]
PHST- 2018/11/30 06:00 [entrez]
AID - S0306-4522(18)30757-7 [pii]
AID - 10.1016/j.neuroscience.2018.11.024 [doi]
PST - ppublish
SO  - Neuroscience. 2019 Jan 15;397:116-126. doi: 10.1016/j.neuroscience.2018.11.024. 
      Epub 2018 Nov 26.