PMID- 30498665
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2190-572X (Print)
IS  - 2190-5738 (Electronic)
IS  - 2190-5738 (Linking)
VI  - 8
IP  - 12
DP  - 2018 Dec
TI  - Highly conserved hemagglutinin peptides of H1N1 influenza virus elicit immune 
      response.
PG  - 492
LID - 10.1007/s13205-018-1509-3 [doi]
LID - 492
AB  - In the current study, two highly conserved (> 90%) H1N1 hemagglutinin peptides 
      STDTVDTVLEKNVTVTHSVNL (H1) and KVNSVIEKMNTQFTAVGKEF (H2) containing multiple 
      T-cell epitopes have been assessed for their immunogenic potential in vitro, 
      subjecting peripheral blood mononuclear cells from healthy volunteers to 
      repetitive stimulation of chemically synthesised H1 and H2 peptides, and 
      measuring their interferon (IFN)-gamma level (ELISA) and proliferation (MTT assay). 
      Further, these peptides were analysed for their binding affinity with 18 
      different human leukocyte antigen (HLA) class I and II by means of molecular 
      docking. All seven samples tested for H1- and H2-induced IFN-gamma secretion were 
      found to have enhanced IFN-gamma production. Six (H1) and five (H2) samples have 
      shown proliferative response compared to unstimulated cells. Peptide-induced 
      IFN-gamma secretion and proliferation in healthy samples represent the immunogenic 
      potential of these peptides. Further, molecular docking results reveal that the 
      peptides have comparable binding energy to that of native bound peptide for both 
      HLA classes which indicates that these peptides have the capability to be 
      presented by different HLA molecules required for T-cell response. Hence, these 
      conserved immunogenic hemagglutinin peptides are potential candidates for 
      influenza vaccine development.
FAU - Lohia, Neha
AU  - Lohia N
AD  - 1Department of Biotechnology, Thapar Institute of Engineering and Technology, 
      147004 Patiala, India. ISNI: 0000 0004 0500 6866. GRID: grid.412436.6
AD  - 2School of Life Sciences, Jaipur National University, Jaipur, India. ISNI: 0000 
      0004 1764 6537. GRID: grid.411809.5
FAU - Baranwal, Manoj
AU  - Baranwal M
AUID- ORCID: 0000-0002-6581-9346
AD  - 1Department of Biotechnology, Thapar Institute of Engineering and Technology, 
      147004 Patiala, India. ISNI: 0000 0004 0500 6866. GRID: grid.412436.6
LA  - eng
PT  - Journal Article
DEP - 20181120
PL  - Germany
TA  - 3 Biotech
JT  - 3 Biotech
JID - 101565857
PMC - PMC6242801
OTO - NOTNLM
OT  - Influenza virus
OT  - Interferon
OT  - Lymphocytes
OT  - Molecular docking
OT  - Peptides
OT  - T-cell-based vaccine
COIS- Compliance with ethical standardsOn behalf of all authors, the corresponding 
      author states that there is no conflict of interest.
EDAT- 2018/12/01 06:00
MHDA- 2018/12/01 06:01
PMCR- 2019/12/01
CRDT- 2018/12/01 06:00
PHST- 2017/11/12 00:00 [received]
PHST- 2018/11/09 00:00 [accepted]
PHST- 2018/12/01 06:00 [entrez]
PHST- 2018/12/01 06:00 [pubmed]
PHST- 2018/12/01 06:01 [medline]
PHST- 2019/12/01 00:00 [pmc-release]
AID - 1509 [pii]
AID - 10.1007/s13205-018-1509-3 [doi]
PST - ppublish
SO  - 3 Biotech. 2018 Dec;8(12):492. doi: 10.1007/s13205-018-1509-3. Epub 2018 Nov 20.