PMID- 30499061
OWN - NLM
STAT- MEDLINE
DCOM- 20190125
LR  - 20190126
IS  - 1559-131X (Electronic)
IS  - 1357-0560 (Print)
IS  - 1357-0560 (Linking)
VI  - 36
IP  - 1
DP  - 2018 Nov 29
TI  - Fluorescence in situ hybridization in 1 mL of selective urine for the detection 
      of upper tract urothelial carcinoma: a feasibility study.
PG  - 10
LID - 10.1007/s12032-018-1237-x [doi]
LID - 10
AB  - Kidney-sparing surgery of upper tract urothelial carcinoma (UTUC) requires a 
      stringent follow-up with frequent ureteroscopies. Triage testing could reduce the 
      number of follow-up ureteroscopies and hence minimize the invasiveness of 
      follow-up. The use of urine-based markers for triage seems appealing but should 
      be feasible with selective urine from outpatient cystoscopy to maximize the 
      reduction of invasiveness. In this study, the feasibility of UroVysion((R)) 
      fluorescence in situ hybridization (FISH) for the detection of UTUC in 1 mL of 
      selective urine is investigated. Ten consecutive patients with biopsy-proven UTUC 
      and five patients with negative diagnostic ureteroscopy findings were included in 
      this case-control study. During ureteroscopy, 1 mL of selective urine was 
      collected passively with a ureteral splint for Urovysion(R) FISH. The FISH rater 
      was blinded to any clinical information. The results of FISH were compared to the 
      findings of concomitantly collected selective urine cytology and the patients' 
      UTUC status. FISH was feasible in all samples with a sensitivity of 90% and a 
      specificity of 80% for UTUC. In comparison, selective cytology resulted in a 
      diagnostic yield of 87% with a sensitivity of 80% and a specificity of 67%. In 
      conclusion, UTUC detection is feasible with FISH in 1 mL of passively collected 
      selective urine. Thus from a technical point of view, FISH could be used as an 
      outpatient triage test to decide if follow-up ureteroscopy is necessary after 
      kidney-sparing surgery of UTUC. Evaluation of the diagnostic accuracy of FISH for 
      the suggested pathway deserves further attention.
FAU - Freund, J E
AU  - Freund JE
AUID- ORCID: 0000-0001-9296-1148
AD  - Department of Urology, Amsterdam University Medical Centers, University of 
      Amsterdam, Meibergdreef 9 G4-223, 1105AZ, Amsterdam, The Netherlands. 
      j.e.freund@amc.nl.
FAU - Liem, E I M L
AU  - Liem EIML
AD  - Department of Urology, Amsterdam University Medical Centers, University of 
      Amsterdam, Meibergdreef 9 G4-223, 1105AZ, Amsterdam, The Netherlands.
FAU - Savci-Heijink, C D
AU  - Savci-Heijink CD
AD  - Department of Pathology, Amsterdam University Medical Centers, University of 
      Amsterdam, Meibergdreef 9, 1105AZ, Amsterdam, The Netherlands.
FAU - de Reijke, T M
AU  - de Reijke TM
AD  - Department of Urology, Amsterdam University Medical Centers, University of 
      Amsterdam, Meibergdreef 9 G4-223, 1105AZ, Amsterdam, The Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20181129
PL  - United States
TA  - Med Oncol
JT  - Medical oncology (Northwood, London, England)
JID - 9435512
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Transitional Cell/*diagnosis/*urine
MH  - Case-Control Studies
MH  - Feasibility Studies
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Urologic Neoplasms/*diagnosis/*urine
PMC - PMC6267383
OTO - NOTNLM
OT  - Cystoscopy
OT  - Fluorescence in situ hybridization
OT  - Upper tract urothelial carcinoma
OT  - Ureteroscopy
OT  - Urine cytology
OT  - Urothelial carcinoma
COIS- CONFLICT OF INTEREST: All authors declare that they have no conflict of interest. 
      INFORMED CONSENT AND ETHICAL APPROVAL: The institutional review board granted a 
      waiver for this study as no additional activities in human subjects were 
      involved. All procedures performed in studies involving human participants were 
      in accordance with the ethical standards of the institutional and/or national 
      research committee and with the 1964 Helsinki declaration and its later 
      amendments or comparable ethical standards.
EDAT- 2018/12/01 06:00
MHDA- 2019/01/27 06:00
PMCR- 2018/11/29
CRDT- 2018/12/01 06:00
PHST- 2018/11/12 00:00 [received]
PHST- 2018/11/26 00:00 [accepted]
PHST- 2018/12/01 06:00 [entrez]
PHST- 2018/12/01 06:00 [pubmed]
PHST- 2019/01/27 06:00 [medline]
PHST- 2018/11/29 00:00 [pmc-release]
AID - 10.1007/s12032-018-1237-x [pii]
AID - 1237 [pii]
AID - 10.1007/s12032-018-1237-x [doi]
PST - epublish
SO  - Med Oncol. 2018 Nov 29;36(1):10. doi: 10.1007/s12032-018-1237-x.