PMID- 30500841 OWN - NLM STAT- MEDLINE DCOM- 20190416 LR - 20200309 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 13 IP - 11 DP - 2018 TI - Sporosarcina pasteurii can clog and strengthen a porous medium mimic. PG - e0207489 LID - 10.1371/journal.pone.0207489 [doi] LID - e0207489 AB - The bacterium Sporosarcina pasteurii can produce significant volumes of solid precipitation in the presence of specific chemical environments. These solid precipitate particles can enter a network of microscale pores and cause long-range clogging. As a result, the medium gains strength and exhibits superior mechanical properties. This concept is also known as Microbiologically Induced Calcite Precipitation (MICP). In this study, we have used sponge blocks as surrogate porous media mimics and analyzed several aspects of MICP. A synergistic approach involving electron microscopy (SEM), computerized X-Ray tomography (muCT), quasi-static compressive load testing and chemical characterization (EDX) has been used to understand several physical and chemical aspects of MICP. FAU - Bhaduri, Swayamdipta AU - Bhaduri S AUID- ORCID: 0000-0002-3001-9007 AD - Ingenuity Lab, Faculty of Engineering, University of Alberta, Edmonton, Alberta, Canada. AD - Department of Mechanical Engineering, University of Alberta, Edmonton, Alberta, Canada. FAU - Montemagno, Carlo AU - Montemagno C AD - Ingenuity Lab, Faculty of Engineering, University of Alberta, Edmonton, Alberta, Canada. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20181130 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - H0G9379FGK (Calcium Carbonate) SB - IM MH - Calcium Carbonate/*chemistry MH - Flocculation MH - Porosity MH - Sporosarcina/*growth & development PMC - PMC6267956 COIS- The authors have declared that no competing interests exist. EDAT- 2018/12/01 06:00 MHDA- 2019/04/17 06:00 PMCR- 2018/11/30 CRDT- 2018/12/01 06:00 PHST- 2018/05/22 00:00 [received] PHST- 2018/10/30 00:00 [accepted] PHST- 2018/12/01 06:00 [entrez] PHST- 2018/12/01 06:00 [pubmed] PHST- 2019/04/17 06:00 [medline] PHST- 2018/11/30 00:00 [pmc-release] AID - PONE-D-18-15369 [pii] AID - 10.1371/journal.pone.0207489 [doi] PST - epublish SO - PLoS One. 2018 Nov 30;13(11):e0207489. doi: 10.1371/journal.pone.0207489. eCollection 2018.