PMID- 30501187 OWN - NLM STAT- MEDLINE DCOM- 20200728 LR - 20200728 IS - 1520-6882 (Electronic) IS - 0003-2700 (Linking) VI - 91 IP - 1 DP - 2019 Jan 2 TI - Facile Preparation of a Bacteria Imprinted Artificial Receptor for Highly Selective Bacterial Recognition and Label-Free Impedimetric Detection. PG - 1027-1033 LID - 10.1021/acs.analchem.8b04314 [doi] AB - The effective identification and quantification of pathogenic bacteria is essential for addressing serious public health issues. Here, we demonstrate a simple and universal impedimetric sensor for highly selective and sensitive detection of pathogenic bacteria based on the recognition by a bacteria-imprinted polypyrrole (BIP) film. The BIP film was facilely prepared via one-step electro-polymerization followed by in situ removal of the bacterial template. The film structure is novel with noncavity-like imprinted sites situated at the surface of the polypyrrole (PPy) matrix, which are more accessible for the target bacteria and should enhance the mass transfer and the binding kinetics. A limit of quantitation low to 10(3) CFU/mL was achieved within 1 h for the detection of E. coli O157:H7, which is comparable to the antibody-based assays. Moreover, the sensor displayed remarkable selectivity, especially regarding the specific identification of bacterial serotypes. When employed to analyze E. coli O157:H7 in real drinking water, apple juice, and milk samples, the sensor showed recoveries from 96.0% to 107.9% with relative standard derivations (RSDs) less than 4%. The BIP-based sensing strategy provides a universal approach for specific, selective, and rapid detection of pathogenic bacteria. As compared to conventional biosensors based on biomolecular recognition, this sensor shows clear advantages including easy-of-preparation, robustness, and low cost, which may hold great potential in fields of food/public safety monitoring. FAU - Wu, Jikui AU - Wu J AD - College of Food Science and Technology , Shanghai Ocean University , Shanghai 201306 , China. FAU - Wang, Ruinan AU - Wang R AD - College of Food Science and Technology , Shanghai Ocean University , Shanghai 201306 , China. FAU - Lu, Yunfei AU - Lu Y AD - College of Food Science and Technology , Shanghai Ocean University , Shanghai 201306 , China. FAU - Jia, Min AU - Jia M AD - College of Food Science and Technology , Shanghai Ocean University , Shanghai 201306 , China. FAU - Yan, Juan AU - Yan J AD - College of Food Science and Technology , Shanghai Ocean University , Shanghai 201306 , China. FAU - Bian, Xiaojun AU - Bian X AUID- ORCID: 0000-0002-1750-6433 AD - College of Food Science and Technology , Shanghai Ocean University , Shanghai 201306 , China. AD - Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai) , Ministry of Agriculture , Shanghai 201306 , China. AD - Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation , Shanghai 201306 , China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20181212 PL - United States TA - Anal Chem JT - Analytical chemistry JID - 0370536 RN - 0 (Antibodies, Immobilized) RN - 0 (Polymers) RN - 0 (Pyrroles) RN - 0 (Receptors, Artificial) RN - 30604-81-0 (polypyrrole) SB - IM MH - Antibodies, Immobilized/*chemistry MH - Escherichia coli O157/*isolation & purification MH - Food Inspection MH - Food Microbiology MH - Food Safety MH - Limit of Detection MH - Polymers/*chemistry MH - Public Health MH - Pyrroles/*chemistry MH - Receptors, Artificial/chemical synthesis/*chemistry EDAT- 2018/12/07 06:00 MHDA- 2020/07/29 06:00 CRDT- 2018/12/04 06:00 PHST- 2018/12/07 06:00 [pubmed] PHST- 2020/07/29 06:00 [medline] PHST- 2018/12/04 06:00 [entrez] AID - 10.1021/acs.analchem.8b04314 [doi] PST - ppublish SO - Anal Chem. 2019 Jan 2;91(1):1027-1033. doi: 10.1021/acs.analchem.8b04314. Epub 2018 Dec 12.