PMID- 30501704
OWN - NLM
STAT- MEDLINE
DCOM- 20190228
LR  - 20190228
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 26
IP  - 6
DP  - 2018 Dec
TI  - [Retrospective Analysis of Genetics Abnormalities in Patients with Multiple 
      Myeloma].
PG  - 1681-1687
LID - 10.7534/j.issn.1009-2137.2018.06.017 [doi]
AB  - OBJECTIVE: To explore the characteristics of cytogenetics and molecular genetics 
      in patients with multiple myeloma(MM). METHODS: Fluorescence in situ 
      hybridization(FISH) was used for molecular genetics analysis in 86 cases of newly 
      diagnosed MM, at the same time the chromosome karyotype analysis was performed in 
      20 cases. Specimen were bone marrow cells. RESULTS: FISH detection showed that 68 
      cases of MM (79.07%) had at least one type of the molecular genetic 
      abnormalities. The positive rates of IgH rearrangement, 1q21 amplification, 
      D13S319 deletion, RB1 deletion and.P53 deletion were 62.79%, 26.74%, 24.42% 
      ,13.95% and 1.16%, respectively. The positive rate of IgH was significantly 
      higher than that of any other probes(P<0.01). The positive rate of IgH was 79.41% 
      in 68 cases. Out of which the positive rate of IgH single and combined with 1, 2, 
      3, 4 probes was 59.26%, 24.07%, 11.11%, 5.56% and 0 respectively. The positive 
      rate of IgH only was very signficantly higher than that of combined with any 
      other probes(P<0.01).The positive rate of 1q21 was 33.82% in 68 cases, Out of 
      which the positive rates of 1q21 or combined with 1,2,3,4 probes was 21.74%, 
      43.48%, 21.74%,13.04% and 0 respectively, the 1q21 probe showed positive as 
      combined with other probes(P<0.01), especially with IgH(P<0.05). The positive 
      rates of D13S319 were 30.88% in 68 cases of patients, out of which the positive 
      rates of D13S319 single or combined with 1, 2, 3, 4 probes was 14.29%, 28.57%, 
      42.86%, 14.29% and 0 respectively, the D13S319 combined with other probes 
      appeared more significant positive(P<0.01), especially with 1 or 2 probes (P< 
      0.01). The positive rate of RB1 was 17.65% in 68 cases, the positive rate of RB1 
      singl or combined with 1, 2, 3, 4 probes were 0, 25%, 50%, 25% and 0, the RB1 
      appeared positive always combined with other probes, especially with D13S319 
      probe (P<0.01). The positive rate of P53 was 1.47%, as combined with RB1 and 
      D13S319 probes. The chromosomal karyotyping showed that 3 cases carried abnormal 
      chromosomal and 17 cases carried normal chromosome, Out of which 17 cases showed 
      positive by FISH. There was a significant difference of sensitivity between FISH 
      combined with chromosome karvotyping and single chromosome karvotype (P< 0.01). 
      CONCLUSION: The genetic abnormalies display obvious heterogenicity in MM. The 
      sensitivity of FISH is higher than that of chromosomal karvotyping. If FISH and 
      chromosome karvotyping are combined, the positive rate of abnormality can be 
      raised.
FAU - Wang, Cun-Bang
AU  - Wang CB
AD  - Department of Hematology, Lanzhou General Hospital, Chinese PLA, Lanzhou 730050, 
      Gansu Province, China.
FAU - Wu, Jing
AU  - Wu J
AD  - Department of Hematology, Lanzhou General Hospital, Chinese PLA, Lanzhou 730050, 
      Gansu Province, China.
FAU - Yang, Ke
AU  - Yang K
AD  - Department of Hematology, Lanzhou General Hospital, Chinese PLA, Lanzhou 730050, 
      Gansu Province, China.
FAU - Su, Miao
AU  - Su M
AD  - Department of Hematology, Lanzhou General Hospital, Chinese PLA, Lanzhou 730050, 
      Gansu Province, China.
FAU - Zhang, Hai-Ying
AU  - Zhang HY
AD  - Department of Hematology, Lanzhou General Hospital, Chinese PLA, Lanzhou 730050, 
      Gansu Province, China.
FAU - Pan, Yao-Zhu
AU  - Pan YZ
AD  - Department of Hematology, Lanzhou General Hospital, Chinese PLA, Lanzhou 730050, 
      Gansu Province, China.
FAU - Wu, Tao
AU  - Wu T
AD  - Department of Hematology, Lanzhou General Hospital, Chinese PLA, Lanzhou 730050, 
      Gansu Province, China.
FAU - Xi, Rui
AU  - Xi R
AD  - Department of Hematology, Lanzhou General Hospital, Chinese PLA, Lanzhou 730050, 
      Gansu Province, China.
FAU - Bai, Hai
AU  - Bai H
AD  - Department of Hematology, Lanzhou General Hospital, Chinese PLA, Lanzhou 730050, 
      Gansu Province, China.E-mail:baihai98@tom.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
SB  - IM
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 13
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Multiple Myeloma/genetics
MH  - Retrospective Studies
EDAT- 2018/12/07 06:00
MHDA- 2019/03/01 06:00
CRDT- 2018/12/04 06:00
PHST- 2018/12/04 06:00 [entrez]
PHST- 2018/12/07 06:00 [pubmed]
PHST- 2019/03/01 06:00 [medline]
AID - 1009-2137(2018)06-1681-07 [pii]
AID - 10.7534/j.issn.1009-2137.2018.06.017 [doi]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2018 Dec;26(6):1681-1687. doi: 
      10.7534/j.issn.1009-2137.2018.06.017.