PMID- 30504642
OWN - NLM
STAT- MEDLINE
DCOM- 20190422
LR  - 20200225
IS  - 1347-4405 (Electronic)
IS  - 1342-6311 (Print)
IS  - 1342-6311 (Linking)
VI  - 34
IP  - 1
DP  - 2019 Mar 30
TI  - Eelgrass Sediment Microbiome as a Nitrous Oxide Sink in Brackish Lake Akkeshi, 
      Japan.
PG  - 13-22
LID - 10.1264/jsme2.ME18103 [doi]
AB  - Nitrous oxide (N(2)O) is a powerful greenhouse gas; however, limited information 
      is currently available on the microbiomes involved in its sink and source in 
      seagrass meadow sediments. Using laboratory incubations, a quantitative PCR 
      (qPCR) analysis of N(2)O reductase (nosZ) and ammonia monooxygenase subunit A 
      (amoA) genes, and a metagenome analysis based on the nosZ gene, we investigated 
      the abundance of N(2)O-reducing microorganisms and ammonia-oxidizing prokaryotes 
      as well as the community compositions of N(2)O-reducing microorganisms in in situ 
      and cultivated sediments in the non-eelgrass and eelgrass zones of Lake Akkeshi, 
      Japan. Laboratory incubations showed that N(2)O was reduced by eelgrass sediments 
      and emitted by non-eelgrass sediments. qPCR analyses revealed that the abundance 
      of nosZ gene clade II in both sediments before and after the incubation as higher 
      in the eelgrass zone than in the non-eelgrass zone. In contrast, the abundance of 
      ammonia-oxidizing archaeal amoA genes increased after incubations in the 
      non-eelgrass zone only. Metagenome analyses of nosZ genes revealed that the 
      lineages Dechloromonas-Magnetospirillum-Thiocapsa and Bacteroidetes 
      (Flavobacteriia) within nosZ gene clade II were the main populations in the 
      N(2)O-reducing microbiome in the in situ sediments of eelgrass zones. 
      Sulfur-oxidizing Gammaproteobacteria within nosZ gene clade II dominated in the 
      lineage Dechloromonas-Magnetospirillum-Thiocapsa. Alphaproteobacteria within nosZ 
      gene clade I were predominant in both zones. The proportions of 
      Epsilonproteobacteria within nosZ gene clade II increased after incubations in 
      the eelgrass zone microcosm supplemented with N(2)O only. Collectively, these 
      results suggest that the N(2)O-reducing microbiome in eelgrass meadows is largely 
      responsible for coastal N(2)O mitigation.
FAU - Nakagawa, Tatsunori
AU  - Nakagawa T
AD  - College of Bioresource Sciences, Nihon University.
FAU - Tsuchiya, Yuki
AU  - Tsuchiya Y
AD  - College of Bioresource Sciences, Nihon University.
FAU - Ueda, Shingo
AU  - Ueda S
AD  - College of Bioresource Sciences, Nihon University.
FAU - Fukui, Manabu
AU  - Fukui M
AD  - Institute of Low Temperature Science, Hokkaido University.
FAU - Takahashi, Reiji
AU  - Takahashi R
AD  - College of Bioresource Sciences, Nihon University.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20181201
PL  - Japan
TA  - Microbes Environ
JT  - Microbes and environments
JID - 9710937
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.2.4 (nitrous oxide reductase)
RN  - EC 1.7.3.- (ammonia monooxygenase)
RN  - K50XQU1029 (Nitrous Oxide)
SB  - IM
MH  - Ammonia/metabolism
MH  - Archaea/classification/genetics/isolation & purification/metabolism
MH  - Bacteria/classification/genetics/isolation & purification/metabolism
MH  - Geologic Sediments/*microbiology
MH  - Japan
MH  - Lakes/*microbiology
MH  - Metagenomics
MH  - *Microbiota/genetics
MH  - Nitrous Oxide/*metabolism
MH  - Oxidation-Reduction
MH  - Oxidoreductases/genetics
MH  - *Saline Waters
MH  - *Water Microbiology
MH  - Zosteraceae/*microbiology
PMC - PMC6440730
OTO - NOTNLM
OT  - Flavobacteriia
OT  - amoA
OT  - eelgrass sediments
OT  - nitrous oxide-reducing microbiome
OT  - nosZ
OT  - sulfur-oxidizing Gammaproteobacteria
EDAT- 2018/12/07 06:00
MHDA- 2019/04/23 06:00
PMCR- 2019/03/01
CRDT- 2018/12/04 06:00
PHST- 2018/12/07 06:00 [pubmed]
PHST- 2019/04/23 06:00 [medline]
PHST- 2018/12/04 06:00 [entrez]
PHST- 2019/03/01 00:00 [pmc-release]
AID - 34_13 [pii]
AID - 10.1264/jsme2.ME18103 [doi]
PST - ppublish
SO  - Microbes Environ. 2019 Mar 30;34(1):13-22. doi: 10.1264/jsme2.ME18103. Epub 2018 
      Dec 1.