PMID- 30504708
OWN - NLM
STAT- MEDLINE
DCOM- 20190204
LR  - 20190215
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 156
IP  - 4
DP  - 2018
TI  - Accumulation of Transposable Elements in Autosomes and Giant Sex Chromosomes of 
      Omophoita (Chrysomelidae: Alticinae).
PG  - 215-222
LID - 10.1159/000495199 [doi]
AB  - Coleoptera is the most diverse order among insects, and comparative molecular 
      cytogenetic studies in this group are lacking. The species of Omophoita 
      (Oedionychina) possess a karyotype of 2n = 22 = 10II+X+Y. They are interesting 
      models for evolutionary cytogenetic studies due to giant sex chromosomes which 
      are asynaptic during meiosis. Transposable elements (TEs) confer plasticity and 
      mobility to genomes and are considered hotspots for DNA double-strand breaks and 
      chromosomal rearrangements. The objective of the present study was to verify the 
      role of TEs in the karyotype and in the size expansion of the giant sex 
      chromosomes in Omophoita. Thus, different TEs were characterized in the Omophoita 
      genome and localized in the chromosomes by fluorescence in situ hybridization 
      (FISH). The DNA sequencing data revealed identity with TE families Tc1/Mariner 
      and RTE/L1-56_XT. FISH showed signals of all TEs in the karyotypes and a high 
      accumulation in the sex chromosomes of the 3 Omophoita species analyzed. These 
      data suggest that the genome size expansion and the origin of the giant sex 
      chromosomes of Omophoita are due to an intensive genomic invasion of TEs, as 
      those characterized here as Tc1/Mariner-Ooc and RTE-Ooc. Differences in the 
      chromosomal location of the TEs among the 3 species indicate that they have 
      participated in the karyotype differentiation in Omophoita.
CI  - (c) 2018 S. Karger AG, Basel.
FAU - Rosolen, Lucas A M
AU  - Rosolen LAM
FAU - Vicari, Marcelo R
AU  - Vicari MR
FAU - Almeida, Mara C
AU  - Almeida MC
LA  - eng
PT  - Journal Article
DEP - 20181201
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA Transposable Elements)
SB  - IM
MH  - Animals
MH  - Chromosomes, Insect/*genetics
MH  - Coleoptera/classification/*genetics
MH  - *DNA Transposable Elements
MH  - Evolution, Molecular
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Sequence Analysis, DNA
MH  - Sex Chromosomes
OTO - NOTNLM
OT  - Asynaptic segregation
OT  - Chromosomal evolution
OT  - Meiotic segregation
OT  - Repetitive DNAs
OT  - Sex chromosomes
OT  - Transposons
EDAT- 2018/12/07 06:00
MHDA- 2019/02/05 06:00
CRDT- 2018/12/04 06:00
PHST- 2018/06/07 00:00 [accepted]
PHST- 2018/12/07 06:00 [pubmed]
PHST- 2019/02/05 06:00 [medline]
PHST- 2018/12/04 06:00 [entrez]
AID - 000495199 [pii]
AID - 10.1159/000495199 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2018;156(4):215-222. doi: 10.1159/000495199. Epub 2018 Dec 
      1.