PMID- 30507567
OWN - NLM
STAT- MEDLINE
DCOM- 20190110
LR  - 20190110
IS  - 1875-8622 (Electronic)
IS  - 1386-0291 (Linking)
VI  - 70
IP  - 3
DP  - 2018
TI  - Characterization of thoracal fat depots - expression of adipokines and remodeling 
      factors and impact of adipocyte conditioned media in fibroblast scratch assays.
PG  - 267-280
LID - 10.3233/CH-170341 [doi]
AB  - Adipose tissue is not only a connective tissue but also an endocrine organ 
      secreting adipokines like Leptin and Adiponectin, lipokines such as palmitoileic 
      acid and extracellular vesicles. These factors and the expression of matrix 
      remodeling enzymes impact surrounding tissues via paracrine effects. The 
      expression of selected secretion factors and the effect of adipocyte conditioned 
      media from four thoracal adipose tissue origins - subcutaneous, perivascular, 
      pericardial and epicardial adipose tissues - in a fibroblast proliferation/wound 
      healing scratch assay model were investigated. Results were compared directly and 
      according to the type 2 diabetic mellitus (T2DM) status of the patients the 
      tissues are originated from. Adipocyte conditioned media from non-diabetic 
      patients resulted in a significant higher scratch closure rate compared to the 
      media with T2DM background. Linoleic acid incubation in scratch assay resulted in 
      a reduced scratch closure rate. Leptin, Adiponectin and Visfatin/Nampt expression 
      and MMP2, MMP9 and FSTL1 mRNA levels did not vary according to T2DM subgroups 
      directly, leading to the assumption that these factors are not causal for scratch 
      assay effects observed. In contrast significant mRNA expression differences were 
      monitored between the thoracal tissue origins implying variations in the local 
      effects of the different adipose tissue depots.
FAU - Dittfeld, C
AU  - Dittfeld C
AD  - Department of Cardiac Surgery, Faculty of Medicine Carl Gustav Carus, Technische 
      Universitat Dresden, Herzzentrum Dresden, Germany.
FAU - Bienger, K
AU  - Bienger K
AD  - Department of Cardiac Surgery, Faculty of Medicine Carl Gustav Carus, Technische 
      Universitat Dresden, Herzzentrum Dresden, Germany.
FAU - Andres, J
AU  - Andres J
AD  - Department of Cardiac Surgery, Faculty of Medicine Carl Gustav Carus, Technische 
      Universitat Dresden, Herzzentrum Dresden, Germany.
FAU - Plotze, K
AU  - Plotze K
AD  - Department of Cardiac Surgery, Faculty of Medicine Carl Gustav Carus, Technische 
      Universitat Dresden, Herzzentrum Dresden, Germany.
FAU - Jannasch, A
AU  - Jannasch A
AD  - Department of Cardiac Surgery, Faculty of Medicine Carl Gustav Carus, Technische 
      Universitat Dresden, Herzzentrum Dresden, Germany.
FAU - Waldow, T
AU  - Waldow T
AD  - Department of Cardiac Surgery, Faculty of Medicine Carl Gustav Carus, Technische 
      Universitat Dresden, Herzzentrum Dresden, Germany.
FAU - Tugtekin, S M
AU  - Tugtekin SM
AD  - Department of Cardiac Surgery, Faculty of Medicine Carl Gustav Carus, Technische 
      Universitat Dresden, Herzzentrum Dresden, Germany.
FAU - Matschke, K
AU  - Matschke K
AD  - Department of Cardiac Surgery, Faculty of Medicine Carl Gustav Carus, Technische 
      Universitat Dresden, Herzzentrum Dresden, Germany.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Clin Hemorheol Microcirc
JT  - Clinical hemorheology and microcirculation
JID - 9709206
RN  - 0 (Adipokines)
SB  - IM
MH  - Adipocytes/*metabolism
MH  - Adipokines/*metabolism
MH  - Aged
MH  - Diabetes Mellitus, Type 2/*complications
MH  - Fibroblasts/*metabolism
MH  - Humans
MH  - Subcutaneous Fat/*metabolism
OTO - NOTNLM
OT  - MMP
OT  - Thoracal adipose tissue depots
OT  - adipokines
OT  - fatty acids
OT  - scratch wound healing assay
OT  - type 2 diabetes mellitus
EDAT- 2018/12/07 06:00
MHDA- 2019/01/11 06:00
CRDT- 2018/12/04 06:00
PHST- 2018/12/04 06:00 [entrez]
PHST- 2018/12/07 06:00 [pubmed]
PHST- 2019/01/11 06:00 [medline]
AID - CH170341 [pii]
AID - 10.3233/CH-170341 [doi]
PST - ppublish
SO  - Clin Hemorheol Microcirc. 2018;70(3):267-280. doi: 10.3233/CH-170341.