PMID- 30509070 OWN - NLM STAT- MEDLINE DCOM- 20200810 LR - 20200810 IS - 1520-6882 (Electronic) IS - 0003-2700 (Linking) VI - 91 IP - 3 DP - 2019 Feb 5 TI - On-Site Ultrasensitive Detection Paper for Multiclass Chemical Contaminants via Universal Bridge-Antibody Labeling: Mycotoxin and Illegal Additives in Milk as an Example. PG - 1968-1973 LID - 10.1021/acs.analchem.8b04290 [doi] AB - Multiclass chemical contamination of food has aroused ever-increasing attention due to the increasingly common findings of the co-occurrence of multiclass contamination, such as mycotoxin (aflatoxin M1, AFM1) and illegal additive (melamine, MEL). In the present study, a rapid, ultrasensitive detection paper was developed on the basis of a unique bridge-antibody label to realize on-site simultaneous detection of AFM1 and MEL in milk. This detection paper used the bridge-antibody label on fluorescent particles (i.e., the fluorescent Eu nanoparticles were first conjugated with polyclonal antibodies and then with monoclonal antibodies). Dramatically enhanced sensitivity was found, probably due to the increase in immobilization of efficient monoclonal antibodies onto microspheres. Under optimal conditions, the lower limits of detection were 0.009 and 0.024 ng/mL for AFM1 and MEL in milk, respectively, in comparison with similar works. Moreover, the cutoff values were 0.4 and 150 ng/mL for AFM1 and MEL, respectively. The recoveries ranged from 88.7% to 105.0% for AFM1 and from 84.6% to 117.7% for MEL, with relative standard deviations (RSDs) of 0.5-9.9% during the intraday and interday experiments. Comparison experiments conducted using the detection paper, HPLC, and UPLC-MS/MS found excellent agreement in the simultaneous detection of AFM1 and MEL in milk. This proposed method can be extensively employed for simultaneous monitoring of multiclass chemical contaminants to ensure food safety. FAU - Li, Guanghua AU - Li G AD - Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences , Wuhan 430062 , People's Republic of China. FAU - Xu, Lin AU - Xu L AD - Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences , Wuhan 430062 , People's Republic of China. FAU - Wu, Wenqin AU - Wu W AD - Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences , Wuhan 430062 , People's Republic of China. FAU - Wang, Du AU - Wang D AD - Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences , Wuhan 430062 , People's Republic of China. AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops , Ministry of Agriculture , Wuhan 430062 , People's Republic of China. AD - Key Laboratory of Detection for Mycotoxins , Ministry of Agriculture , Wuhan 430062 , People's Republic of China. AD - Natonal Reference for Biotoxin Detection , Wuhan 430062 , People's Republic of China. FAU - Jiang, Jun AU - Jiang J AD - Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences , Wuhan 430062 , People's Republic of China. AD - Key Laboratory of Detection for Mycotoxins , Ministry of Agriculture , Wuhan 430062 , People's Republic of China. FAU - Chen, Xiaomei AU - Chen X AD - Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences , Wuhan 430062 , People's Republic of China. FAU - Zhang, Wen AU - Zhang W AD - Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences , Wuhan 430062 , People's Republic of China. AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops , Ministry of Agriculture , Wuhan 430062 , People's Republic of China. AD - Key Laboratory of Detection for Mycotoxins , Ministry of Agriculture , Wuhan 430062 , People's Republic of China. AD - Natonal Reference for Biotoxin Detection , Wuhan 430062 , People's Republic of China. FAU - Poapolathep, Saranya AU - Poapolathep S AD - Department of Pharmacology, Faculty of Veterinary Medicine , Kasetsart University , Bangkok 10900 , Thailand. FAU - Poapolathep, Amnart AU - Poapolathep A AD - Department of Pharmacology, Faculty of Veterinary Medicine , Kasetsart University , Bangkok 10900 , Thailand. FAU - Zhang, Zhaowei AU - Zhang Z AUID- ORCID: 0000-0002-6686-2995 AD - Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences , Wuhan 430062 , People's Republic of China. FAU - Zhang, Qi AU - Zhang Q AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops , Ministry of Agriculture , Wuhan 430062 , People's Republic of China. AD - Key Laboratory of Detection for Mycotoxins , Ministry of Agriculture , Wuhan 430062 , People's Republic of China. AD - Natonal Reference for Biotoxin Detection , Wuhan 430062 , People's Republic of China. FAU - Li, Peiwu AU - Li P AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops , Ministry of Agriculture , Wuhan 430062 , People's Republic of China. AD - Key Laboratory of Detection for Mycotoxins , Ministry of Agriculture , Wuhan 430062 , People's Republic of China. AD - Natonal Reference for Biotoxin Detection , Wuhan 430062 , People's Republic of China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20181220 PL - United States TA - Anal Chem JT - Analytical chemistry JID - 0370536 RN - 0 (Antibodies) RN - 0 (Food Additives) RN - 0 (Illicit Drugs) RN - 6795-23-9 (Aflatoxin M1) SB - IM MH - Aflatoxin M1/*analysis MH - Animals MH - Antibodies/*chemistry MH - Food Additives/*analysis MH - Food Contamination/*analysis MH - Food Safety MH - Illicit Drugs/*analysis MH - Milk/*chemistry MH - *Paper EDAT- 2018/12/05 06:00 MHDA- 2020/08/11 06:00 CRDT- 2018/12/05 06:00 PHST- 2018/12/05 06:00 [pubmed] PHST- 2020/08/11 06:00 [medline] PHST- 2018/12/05 06:00 [entrez] AID - 10.1021/acs.analchem.8b04290 [doi] PST - ppublish SO - Anal Chem. 2019 Feb 5;91(3):1968-1973. doi: 10.1021/acs.analchem.8b04290. Epub 2018 Dec 20.